Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method for solid high temperature resistant acidic mannase

A technology of mannanase and preparation method, which is applied in the preparation field of solid high temperature resistant acid mannanase, can solve the problems of unseen fermentation production, extraction and manufacturing, etc.

Active Publication Date: 2013-04-24
JINANBESTZYME BIO ENG CO LTD
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it is also reported that mannanase is fermented by Aspergillus niger solid state, its actual fermentation production and extraction production have not been seen.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method for solid high temperature resistant acidic mannase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Soaking: Mix the rhizopus solid fermented koji material with water at a ratio of 1:10, adjust the pH of the soaking water to 4.5 with acetic acid, and stir for 2.6 hours;

[0022] Centrifugation: Centrifuge in a three-legged centrifuge for 15 minutes at a speed of 800 rpm, and take the supernatant after centrifugation;

[0023] Plate and frame filtration: add 10% diatomaceous earth, filter with a plate and frame filter to obtain the filtrate;

[0024] Ultrafiltration concentration: membrane molecular weight 10000, concentrated to 1 / 10 of the filtrate volume;

[0025] Spray drying: According to the ratio of concentrated solution: corn starch: sodium carboxymethyl cellulose = 1:0.2:0.03, stir evenly, the inlet air temperature of spray drying: 180°C, the outlet air temperature: 80°C, the final moisture content of the material: 5.9%.

[0026] source of material

[0027] Koji material: fermented from Rhizopus solid

[0028] Detection method used in the present invention-...

Embodiment 2

[0107] Soaking: Mix the rhizopus solid fermented koji material with water at a ratio of 1:16, adjust the pH of the soaking water to 4.5 with acetic acid, and stir for 2 hours;

[0108] Centrifugation: Centrifuge in a three-legged centrifuge for 12 minutes at a speed of 1000 rpm, and take the supernatant after centrifugation;

[0109] Plate and frame filtration: add 8% diatomaceous earth, filter with a plate and frame filter to obtain the filtrate;

[0110] Ultrafiltration concentration: membrane molecular weight 10000, concentrated to 1 / 12 of the filtrate volume;

[0111] Spray drying: According to the ratio of concentrated liquid: corn starch: sodium carboxymethyl cellulose = 1:0.2:0.03, stir evenly, the inlet air temperature of spray drying: 170°C, the outlet air temperature: 76°C, the final moisture content of the material: 6.2%.

Embodiment 3

[0113] Soaking: Mix the rhizopus solid fermented koji material with water at a ratio of 1:8, adjust the pH of the soaking water to 4.5 with acetic acid, and stir for 3 hours;

[0114]Centrifugation: Centrifuge in a three-legged centrifuge for 12 minutes at a speed of 1200 rpm, and take the supernatant after centrifugation;

[0115] Plate and frame filtration: add 7% diatomaceous earth, filter with a plate and frame filter to obtain the filtrate;

[0116] Ultrafiltration concentration: membrane molecular weight 10000, concentrated to 1 / 8 of the filtrate volume;

[0117] Spray drying: According to the ratio of concentrated solution: corn starch: sodium carboxymethyl cellulose = 1:0.2:0.03, stir evenly, the inlet air temperature of spray drying: 160°C, the outlet air temperature: 70°C, the final moisture content of the material: 7.0%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of enzyme preparations and particularly relates to a preparation method for solid high temperature resistant acidic mannose. The method comprises the following steps: soaking rhizopus solid fermenting leaven; centrifuging the mixed liquid; removing residues; filtering; performing ultrafiltration concentration on the filtrate to obtain concentrated liquid; and spray drying the concentrated liquid to obtain the liquid mannose. Compared with the common method for preparing the mannose by fermenting Pichia pastoris flow and methanol liquid, the method has the beneficial effects that the mannose produced by the method has high acid resistance and high heat resistance in the aspects of acid resistance and heat resistance.

Description

technical field [0001] The invention belongs to the field of enzyme preparations, and in particular relates to a preparation method of solid high-temperature resistant acid mannanase. Background technique [0002] Mannanase (endo-1,4-β-mannanase) is a new type of enzyme preparation, which belongs to a kind of hemicellulase. It has the function of general non-starch polysaccharide (NSP) enzymes - degrading NSP , reduce intestinal viscosity, promote the digestion and absorption of nutrients; many recent studies have shown that mannanase is also a multifunctional growth-promoting agent, which can promote the secretion of insulin-like growth factor IGF-I and promote protein synthesis. At the same time, it can also eliminate the interference of β-mannan rich in beans on glucose absorption, and greatly improve the energy digestibility of cakes, especially soybean meal. In actual use, it can also be seen that the resistance and uniformity of animals are improved after adding manna...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/42C12R1/845
Inventor 郭芳坤
Owner JINANBESTZYME BIO ENG CO LTD