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Molecularly imprinted membrane electrode based on nucleic acid adapters for detecting organic arsenide in marine product and preparation method of electrode

A technology of molecularly imprinted membrane and nucleic acid aptamer, which is used in measurement devices, analytical materials, and material analysis by electromagnetic means, can solve problems that have not yet been seen, and achieve the effects of light weight, good repeatability and small size.

Inactive Publication Date: 2012-07-18
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there have been no relevant reports on a molecularly imprinted membrane electrode based on nucleic acid aptamers for the detection of organic arsenic in seafood made by screen printing technology.

Method used

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  • Molecularly imprinted membrane electrode based on nucleic acid adapters for detecting organic arsenide in marine product and preparation method of electrode
  • Molecularly imprinted membrane electrode based on nucleic acid adapters for detecting organic arsenide in marine product and preparation method of electrode

Examples

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preparation example Construction

[0037] A method for preparing a molecularly imprinted membrane electrode of the present invention, comprising the following steps: on an electrode substrate made by screen printing technology, spraying conductive materials to form a working electrode, a reference electrode and a counter electrode The substrates are coated with a layer of polycarbonate on the surface of their middle parts, and the reaction layer formed by surface modification technology is coated on the lower part of the working electrode substrate, that is, the molecular imprinted membrane electrode based on nucleic acid aptamers is made. The preparation method is as follows:

[0038] a, followed by Al 2 o 3 powder to polish the working electrode, and then use HNO with a mass fraction of 50% 3 Carry out treatment, finally fully rinse with acetone and twice distilled water respectively;

[0039] b. Preparation of organic arsenide molecularly imprinted polymers in seafood based on nucleic acid aptamers: the m...

Embodiment 2

[0051] The conductive material used as the substrate of the working electrode and the counter electrode is carbon, and the others are the same as in Example 1.

Embodiment 3

[0053] The conductive material used as the substrate of the working electrode and the counter electrode is gold, and the others are the same as in Example 1.

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Abstract

The invention discloses a molecularly imprinted membrane electrode based on nucleic acid adapters for detecting organic arsenide in a marine product and a preparation method of the electrode. The molecularly imprinted membrane electrode comprises an electrode substrate; a first electrode matrix, a second electrode matrix and a third electrode matrix are arranged on the electrode substrate; the first electrode matrix is connected with a first connecting terminal into a whole by a working electrode; the second electrode matrix is connected with a second connecting terminal into a whole by a counter electrode; the third electrode matrix is connected with a third connecting terminal into a whole by a reference electrode; and a responding layer is coated on the surface of the working electrode, and the responding layer consists of a nucleic acid adapter molecularly imprinted polymer which is fixed on the working electrode by adopting a surface modification technology and can generate specific molecular recognition biochemical reaction with the organic arsenide in the marine product. The preparation method is simple, the electrode is stable in performance and good in repeatability, and the method is suitable for industrialized practical application of organic arsenide detection biosensors in the marine product.

Description

technical field [0001] The invention relates to a biochemical electrode for analyzing materials by testing electrochemical variables and a preparation method thereof, in particular to a nucleic acid aptamer-based molecular imprinted membrane electrode for detecting organic arsenic in seafood and a preparation method thereof. Background technique [0002] Arsenic contamination of seafood has always been the focus of attention in the field of food safety. Unlike terrestrial organisms, marine organisms generally have a strong ability to accumulate arsenic. Fish, shellfish, seaweed and other seafood that humans often eat contain high concentrations of arsenic. Arsenic in seafood mainly exists in the form of organic arsenic compounds (such as methylarsinic acid, methylarsinic acid, arsenoic sugar, arsenocholine, etc.), accounting for about 95% of the total arsenic. At present, the toxicity of arsenosugar and arsenocholine has not been confirmed, but methylarsinic acid has been c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 刘素黄加栋颜梅于京华葛慎光邢宪荣廉文静崔敏李杰
Owner UNIV OF JINAN