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Method for establishing prawn virus infection model

A technology for virus infection and establishment of methods, applied in the field of anti-virus research, can solve problems affecting the reproducibility and reliability of experimental results, and achieve the effects of clear judgment standards, high reliability, and easy operation

Inactive Publication Date: 2012-08-01
EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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AI Technical Summary

Problems solved by technology

Conducting RNAi anti-virus experiments under such conditions will inevitably affect the reproducibility and reliability of the experimental results

Method used

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  • Method for establishing prawn virus infection model
  • Method for establishing prawn virus infection model

Examples

Experimental program
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Effect test

Embodiment 1

[0021] WSSV virus isolation and preparation of virus preservation solution

[0022] The appendages, carapace and hepatopancreas were removed from the dead Litopenaeus vannamei after artificial inoculation with WSSV and weighed, diluted with 0.01M PBS (pH 7.4) 1:10 (g / mL), homogenized with a glass homogenizer , the homogenate was centrifuged at 4000rpm at 4°C for 20min, and the supernatant was sterilized by filter paper and 0.45μm filter membrane successively, and aliquoted into virus preservation solution, which was frozen at -80°C for later use.

[0023] Infection of Litopenaeus vannamei with different concentrations of WSSV preservation solution

[0024] Newly purchased healthy Litopenaeus vannamei with a body weight of 7-9 g and a negative PCR test were temporarily raised in an indoor pond for 1 week, and then randomly divided into groups of 14 in each group, which were temporarily raised in 90L white glass fiber buckets. Dilute the virus preservation solution to 10 -1 、1...

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Abstract

The invention relates to a method for establishing a prawn virus infection model. The method comprises the following steps of: (1) preparing white spot syndrome virus (WSSV) separation and preserving fluid; (2) infecting litopenaeus vannamei with the WSSV preserving fluid at different concentration; and (3) determining the prawn virus infection quantity in the current ribonucleic acid (RNA) interference study by a prawn cumulative death rate observation method. The prawn virus infection model established by the method is high in reproducibility and credibility of RNA interference study results; and the method is easy and convenient to operate and clear in judgment standard, is suitable for the antiviral study of RNA interference and also can be used for the popularization and application of RNA interference preparations in aquatic product practice.

Description

technical field [0001] The invention belongs to the field of anti-virus research, in particular to a method for establishing a shrimp virus infection model. Background technique [0002] Prawns have strong reproductive ability, rapid growth, and delicious meat. Litopenaeus vannamei has a wide range of salinity and strong disease resistance, so it has been cultivated on a large scale in the world. More than 80% of the cultured prawn species in my country are Litopenaeus vannamei. [0003] Shrimp white spot syndrome virus (WSSV) ranks first among the shrimp viruses because of its pantropicity, high pathogenicity and high lethality to the host. Infected shrimp generally have a mortality rate of up to 100% within 3 to 10 days. Because shrimp, like other invertebrates, lacks specific immune mechanism, the prevention and treatment of viral diseases has always been based on non-specific prevention, lacking specific control methods. Therefore, WSSV brings huge economic losses to...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 周俊芳房文红万夕和
Owner EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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