Method for establishing prawn virus infection model
A technology for virus infection and establishment of methods, applied in the field of anti-virus research, can solve problems affecting the reproducibility and reliability of experimental results, and achieve the effects of clear judgment standards, high reliability, and easy operation
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[0020] Example 1
[0021] WSSV virus isolation and preparation of virus preservation solution
[0022] The appendages, carapace and hepatopancreas were removed from the dead Litopenaeus vannamei after artificial inoculation with WSSV and weighed, diluted with 0.01M PBS (pH 7.4) 1:10 (g / mL), homogenized with a glass homogenizer , the homogenate was centrifuged at 4000rpm at 4°C for 20min, and the supernatant was sterilized by filter paper and 0.45μm filter membrane successively, and aliquoted into virus preservation solution, which was frozen at -80°C for later use.
[0023] Infection of Litopenaeus vannamei with different concentrations of WSSV preservation solution
[0024] Newly purchased healthy Litopenaeus vannamei with a body weight of 7-9 g and a negative PCR test were temporarily raised in an indoor pond for 1 week, and then randomly divided into groups of 14 in each group, which were temporarily raised in 90L white glass fiber buckets. Dilute the virus preservation s...
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