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Nano-silver fluorescent probe for detection of cyanide ion

A technology of fluorescent probes and cyanide ions, applied in the field of fluorescent probes, can solve the problems of low spectral detection sensitivity and achieve the effects of low detection concentration, high detection sensitivity and simple preparation method

Inactive Publication Date: 2012-08-08
SHANXI UNIV
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Problems solved by technology

The spectral method is simple and easy to implement, but the detection sensitivity of the visible spectrum is much lower than that of the fluorescence spectral method
There are a lot of studies on organic matter as probes in fluorescence spectroscopy, but most of the reported measurement conditions are organic solvents, and there is no report on the detection of cyanide ions using nano-silver as a fluorescent probe.

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  • Nano-silver fluorescent probe for detection of cyanide ion
  • Nano-silver fluorescent probe for detection of cyanide ion

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Embodiment Construction

[0008] Dissolve the oligonucleotide in 10 mL of deionized water to a concentration of 2.0 x 10 -5 mol / L, add 120uL 1.0×10 to 10mL oligonucleotide aqueous solution -2 mol / L silver nitrate, so that the final concentration of silver nitrate is 1.2×10 -4 mol / L, under electromagnetic stirring, add 120uL 1.0×10 -2 mol / L sodium borohydride aqueous solution; react at room temperature for 4 hours to obtain a water-soluble nano-silver fluorescent probe. The oligonucleotide is 5'-AAAAAAAACCCCCCCCCTTTTTTTT-3'.

[0009] The nano-silver fluorescent probe is dissolved in a tri-acid buffer solution with pH=9, and then a solution containing cyanide ions is added, and tested by a fluorescence spectrophotometer. see results figure 1 with figure 2 , the probe of the present invention is excited at 568nm and emitted at 632nm. Cyanide ion concentrations were tested in the range of 2.5nM to 75nM.

[0010] The nano-silver fluorescent probe of the present invention has high detection sensitivi...

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Abstract

The invention provides a nano-silver fluorescent probe for detection of cyanide ion. The nano-silver fluorescent probe is prepared through the following step: oligonucleotide is use as a template to react for at room temperature for 4 hours under the effect of a reducing agent sodium borohydride, so as to obtain a water-soluble nano-silver fluorescent probe. The prepared nano-silver fluorescent probe is excitated at 568nm and emits at 632nm, the fluorescent probe has sensitive selectivity to cyanide ion and can detect a minimum cyanide concentration of 2.5nM.

Description

technical field [0001] The invention relates to a fluorescent probe, in particular to a water-soluble nano-silver fluorescent probe for detecting cyanide ions and a synthesis method thereof. Background technique [0002] Cyanide is dauntingly toxic, and generally does not contain cyanide in natural water. Cyanide mainly comes from industrial pollution. After cyanide enters the body, it decomposes into toxic cyanide ions (CN - ), cyanide ions can inhibit the activity of 42 enzymes in tissue cells, such as cytochrome oxidase, peroxidase, decarboxylase, succinate dehydrogenase and lactate dehydrogenase. Among them, cytochrome oxidase is the most sensitive to cyanide. CN in drinking water allowed by the World Health Organization - The concentration was 1.9 μM. At present, the methods for determining cyanide ion include chemical sensors, spectroscopic methods, electrochemical sensors, biosensors and so on. The spectroscopic method is simple and easy to implement, but the det...

Claims

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Application Information

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IPC IPC(8): C09K11/58G01N21/64
Inventor 芦冬涛彭秀英李俊芬王兰双少敏董川
Owner SHANXI UNIV
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