Real time detecting method for influence of ultrasonic waves on immobilized peroxidase activity
A technology for real-time detection and enzyme activity, applied in the direction of material electrochemical variables, etc., can solve the problems of insufficient sensitivity, and achieve the effect of high sensitivity and high stability
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0026] Before modification, the surface of the glassy carbon electrode was polished and polished with abrasive cloth covered with 0.05mm alumina paste, and then the glassy carbon electrode was ultrasonically cleaned with acetone, 50% nitric acid, 50% NaOH solution, and double distilled water, respectively. Dry at room temperature.
[0027] Dissolve 2.5 mg 300 U / mL of two peroxidases and 4 mg bovine serum albumin in 0.2 mL of KH 2 PO 4 -Na 2 HPO 4 A mixed solution was formed in phosphate buffered saline (PBS, 0.05mol / L, pH 7.0). Then 20 μL of the mixture was dropped onto the surface of the glassy carbon electrode. The electrodes were then placed in a closed container containing 25% glutaraldehyde vapor for cross-linking for 4 hours, dried at room temperature for 1 hour, and stored at 4°C until use.
[0028]Cyclic voltammetry experiments were performed with an electrochemical workstation and a three-electrode system. Among them, the glassy carbon electrode modified with ho...
Embodiment 2
[0030] Electrochemical experiments to detect the effect of ultrasonic power were carried out in a reaction cell containing 20 mL of supporting electrolyte. Add 1mmol / L hydroquinone and 0.1mL 0.1mol / L H to the electrolyte 2 o 2 solution. A baseline of initial current was recorded at a voltage of -0.25V. Adjust the rotational speed of the stirrer to 400-600 rpm until the reduction current does not rise any more. Then, the reaction pool was placed in a 5L ultrasonic cleaner, the ultrasonic frequency was adjusted to 45kHz, and the ultrasonic radiation with a power of 40, 50, 60, 70, 80, 90 and 100W was used for treatment in sequence, and each power lasted for 100s to obtain a A gradually rising peak-shaped current-time curve.
Embodiment 3
[0032] Electrochemical experiments to detect the effect of ultrasonic frequency were carried out in a reaction cell containing 20 mL of supporting electrolyte. Add 1mmol / L hydroquinone and 0.1mL 0.1mol / L H to the electrolyte 2 o 2 solution. A baseline of initial current was recorded at a voltage of -0.25V. Adjust the rotational speed of the stirrer to 400-600 rpm until the reduction current does not rise any more. Then, place the reaction pool in a 5L ultrasonic cleaner, adjust the ultrasonic power to 100W, and use ultrasonic radiation with frequencies of 20, 45, 80, and 100kHz in sequence for treatment. Each frequency lasts for 100s, and a trend of first rising and then falling is obtained. The peak-shaped current-time curve of the .
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More