Preparation method for laver mycosporine-like amino acids Porphyra-334

An amino acid and bacterial cell technology, applied in the preparation of imino compounds, organic chemistry, etc., can solve the problems of low yield, low yield, and inability to use bacteriocin-like amino acids.

Inactive Publication Date: 2012-09-12
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are mainly two methods for preparing mycosporine-like amino acid (Porphyra-334) from seaweed, one is prepared by high performance liquid chromatography (HPLC) after extraction with polar solution, and the other is after extraction with methanol solution, After simple purification, SephadexG10, DEAE Sephadex A-25, Narit A, CM Sephadex C-25 and other silica gel columns are used for purification. These two methods have low yield and little output, and are suitable for preparation and purification in the laboratory Porphyra-334, but neither can be applied to the preparation of large amounts of mycosporine-like amino acids (Porphyra-334)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: Crude Extraction of Mycosporine-like Amino Acids (Porphyra-334)

[0015] Take 20g of pulverized laver, use 20g~200g (weight ratio) of hydrochloric acid aqueous solution of pH 1~5 to extract respectively 0.5, 1, 1.5, 2, 2.5, 3, 4 hours at 20 ℃, filter, residue (about 18g) with 1 ~5 times (weight ratio 18~90g) of hydrochloric acid aqueous solution of pH 1~5 washes, after filtering, combine supernatant liquid. Adding 50-100% ethanol solution five times the volume of the clear liquid to the clear liquid for precipitation, after filtering, the clear liquid is concentrated at low temperature to obtain a crude mycosporine-like amino acid solution.

[0016] The analysis of the extraction rate of mycosporine-like amino acids (Porphyra-334) showed that the extraction was basically complete after 2 hours of extraction, and the extraction rate did not change significantly if the extraction time was prolonged.

Embodiment 2

[0017] Example 2: Purification by anion exchange resin

[0018] Take 50mL of the crude product solution and pass it through the anion exchange resin D284 (manufactured by Nankai University Chemical Factory, or different types of products from other manufacturers) at 4-40°C, and perform adsorption at a flow rate of 50-500mL / h. After the adsorption is completed, Use 0.005, 0.01, 0.15, 0.2, 0.3, 0.4N NaOH solution for elution, the elution flow rate is 1-10mL / min, and the eluent is concentrated at low temperature.

[0019] The analysis of the elution rate of mycosporine-like amino acids (Porphyra-334) in anion exchange resins shows that 0.01N NaOH solution has a good elution effect, and the concentration is lower than 0.01N. The elution effect is not good, and the yield Low; while the concentration of NaOH solution is too high, it will cause the destruction of mycosporine-like amino acid (Porphyra-334), which reduces the elution rate.

Embodiment 3

[0020] Example 3: Purification by cation exchange resin

[0021] Take 50mL of the solution that has passed through the anion resin, and pass it through the cation exchange resin D113 (manufactured by Nankai University Chemical Factory, or different types of products from other manufacturers) at 4-40°C, and perform adsorption at a flow rate of 50-500mL / h. After the adsorption is completed, use hydrochloric acid solutions of pH 1, 2, 3, and 4 to elute, respectively, at an elution flow rate of 1 to 10 mL / min, and a volume of eluent of 1 to 10 times. The eluate was dried at low temperature to obtain mycosporine-like amino acid (Porphyra-334).

[0022] The analysis of the elution rate of mycosporine-like amino acid (Porphyra-334) showed that the elution effect of hydrochloric acid aqueous solution with pH 2 was the best. Too high or too low will reduce the yield of mycosporin-like amino acid Porphyra-334.

[0023] The detection of the prepared mycosporine-like amino acid (Porphyr...

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Abstract

The invention relates to a preparation method for laver mycosporine-like amino acids (Porphyra-334). The preparation method includes using dry laver as a raw material; extracting by acidic aqueous solution; precipitating by ethanol solution; performing absorption and elution by cation exchange resin and anion exchange resin respectively; and drying so as to obtain a mycosporine-like amino acids (Porphyra-334) product. The prepared mycosporine-like amino acids (Porphyra-334) are high in extraction rate, short in production period, simple in production process, controllable in quality, low in cost and suitable for industrial production. The prepared product is white or faint yellow, is good in quality, is quite easy to be dissolved in water, can be used for the field of medical healthcare, cosmetics, foods and the like, and provides a novel thinking for high-value utilization of laver.

Description

technical field [0001] The invention relates to an ultraviolet absorbing substance (mycosporin-like amino acid), in particular to a method for extracting and preparing a porphyra mycosporine-like amino acid (Porphyra-334). Background technique [0002] Laver is an important economic algae in my country, with an annual output of 750,000 tons. Laver contains a variety of nutrients and is deeply loved by consumers. Seaweed is red algae, which contains a variety of mycosporine-like amino acids, and the content is relatively rich. Mycosporine-like amino acids (MAAs) are a class of small-molecule ultraviolet-absorbing substances contained in seaweed. They are water-soluble compounds. Zones have absorption capacity, they have a very high molar absorptivity coefficient, and are the strongest natural ultraviolet absorbing substances. Such compounds have great potential industrial application value in food, cosmetics and other industries. Porphyra-334 is a kind of mycosporine-like ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C251/20C07C249/02
Inventor 张朝辉高昕许志恒许加超
Owner OCEAN UNIV OF CHINA
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