Shallow culture method for sugarcane liquid

Abstract

The invention relates to a shallow culture method for sugarcane liquid. The method comprises the following steps of: preparing a bacteriostatic agent, disinfecting a culture container, preparing a culture medium, performing inducing culture, multiplication culture and rooting culture, and transplanting bottle seedlings. By the shallow culture method for the sugarcane liquid, the culture medium isnot required to be sterilized under high pressure, and preparation links of the culture medium are simplified; agar powder is eliminated in the culture medium, the proliferation rate is improved by over 29.2 percent, the strong seedling number in each cluster is improved by over 53.9 percent, and the tissue culture cost can be reduced by over 25 percent; and moreover, the thick and strong bottle seedlings are favorable for the transplantation of the bottle seedlings. The invention is characterized in that the method is easy to operate, low in investment, and high in practicability and popularization; and the method can be popularized in the industrial tissue culture seedling cultivation of sugarcane.

Description

technical field [0001] The invention relates to a plant seedling cultivation method, in particular to a sugarcane liquid shallow layer cultivation method, which belongs to the field of biotechnology. Background technique [0002] Sugarcane (Saccharum Complex) is the most important sugar crop in my country, and the sugarcane sugar output accounts for 92% of the total sugar production in my country. Therefore, sucrose production is related to the safety of sugar in my country, and sucrose production depends on the production of raw material sugarcane, and the quality of seedlings is directly related to Affect the yield and quality of raw sugarcane. [0003] Sugarcane tissue culture, on the one hand, can expand its reproduction coefficient to achieve large-scale reproduction; on the other hand, it can realize detoxification and rapid propagation of sugarcane seedlings on the basis of preserving its original excellent traits. The cost of sugarcane tissue culture mainly includes...

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Problems solved by technology

[0004] The invention patent application with the application number 201110311185.4 "An Open Method for Cultivating Virus-free Sugarcane Seedlings" introduces a culture medium that does not require autoclaving, does not require ultra-clean workbenches, and only needs to be inoculated in a natural and open environment The open method of cultivating sugarcane virus-free seedlings fundamentally simplifies the tissue culture process and can reduce the cost of tissue culture by 20%; however, the proliferation coefficient of tissue culture seedlings and the number of strong seedlings / cluster still have limitations.

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