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Eucommia ulmoids leaf extracts and preparation method and application thereof

A technology of eucommia leaves and extracts, applied in the field of eucommia leaf extracts and its preparation, can solve the problems of huge medicinal value, high price, and scarce number of eucommia trees, and achieve the goals of increasing medicinal parts, promoting healing, and reducing costs Effect

Active Publication Date: 2014-04-16
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the scarcity of eucommia trees, and in order to ensure the quality of medicinal materials, eucommia ulmoides must grow to 10 years or more before collecting eucommia bark, and because of the huge medicinal value of eucommia ulmoides, it is expensive

Method used

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  • Eucommia ulmoids leaf extracts and preparation method and application thereof
  • Eucommia ulmoids leaf extracts and preparation method and application thereof
  • Eucommia ulmoids leaf extracts and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The preparation of embodiment 1 eucommia leaf extract

[0021] I. water extract Eucommia leaves and prepare Eucommia leaves crude extract (i.e. Eucommia leaves water extract)

[0022] Eucommia ulmoides leaves (collected from medicinal material plantations in Zhangzhu Town, Yixing City, Wuxi City) are dried, cut into pieces (cutting particle size 1-3mm2), and extracted with water three times. times and 9 times, and the reflux time for each extraction was 2h, 1.5h and 1h in turn. After filtering the three extracts, the filtrates were combined and concentrated to 2.857g crude drug / ml to obtain the crude extract of eucommia leaves.

[0023] II. Ethanol Extraction of Eucommia Leaves to Prepare Eucommia Leaves Crude Extract (i.e. Eucommia Leaf Alcohol Extract)

[0024] Leaves of Eucommia ulmoides (collected from medicinal plantation in Zhangzhu Town, Yixing City, Wuxi City) are dried and shredded (cutting particle size 1-3mm) 2 ), by mass, add 10 times the volume percentage...

Embodiment 2

[0057] Example 2 Method for detecting the content of each component in the refined eucommia leaf extract

[0058] Using HPLC method, the contents of five components in Eucommia ulmoides leaf extract were detected simultaneously, they were: chlorogenic acid, quercetin-3-O-α-L-arabinopyranosyl-(1→2)- β-D-glucosyl glycoside (hereinafter referred to as: DY-1), rutin, isoquercitrin, astragalin. The content of each component in the Eucommia leaf extract sample was calculated by the external standard one-point method.

[0059] 1. Sample preparation

[0060] 1.1 Preparation of mixed reference substance

[0061] Accurately weigh 4.89 mg of chlorogenic acid reference substance, dissolve in ethanol with a volume percentage concentration of 50%, and dilute to 10 ml to obtain a chlorogenic acid reference substance solution with a concentration of 489 μg / ml;

[0062] Accurately weigh 6.43mg of DY-1 reference substance, dissolve it in ethanol with a volume percentage concentration of 50%,...

Embodiment 3

[0083] Example 3 Experimental Study on the Effect of Eucommia Leaf Extract on Promoting the Proliferation Rate of MC3T3-E1 Osteoblasts

[0084] 1. Positive drug concentration screening

[0085] 1.1 Selection of positive control drug

[0086] The positive drug is selected from the commonly used clinical drug Jiegu Qili Tablets (produced by Hunan Jinsha Pharmaceutical Co., Ltd.) for the treatment of fractures.

[0087] 1.2 Preparation of positive control drug sample solution

[0088] Take bone bone seven centimeters, grind it, weigh 0.3g, 20ml of 50% ethanol by volume, sonicate for 10min, and centrifuge at 3000r·min-1 for 10min to obtain an extract with a concentration of 15mg / ml. Accurately draw 0.5ml of supernatant, add 75μl of ethanol with volume percentage concentration of 95% to help dissolve, add 675μl of DMEM high-glucose medium, mix well, and obtain the medium solution of 10mg / ml of bone bone seven centimeter tablet, after Filter through a 0.25 μm microporous membrane...

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Abstract

The invention discloses an eucommia ulmoids leaf water and / or low alcohol extract and preparation method and application thereof, which comprise crude extracts and refined eucommia ulmoids leaf extracts, wherein the crude extracts are extracted from folium cortex eucommiae through water or low alcohol, or are obtained by mixing and extracting the water and the low alcohol, and the refined eucommia ulmoids leaf extracts are obtained after the crude extracts are refined. The eucommia ulmoids leaf water and / or low alcohol extract has obvious effect in application of preparing drugs for promoting fracture healing. The eucommia ulmoids leaf water and / or low alcohol extract can obviously increase alkaline phosphatase, reduces blood calcium, increases serium inorganic phosphorus, increases bone mineral density, and can obviously promote healing of bones after fracturing. Furthermore, medicinal parts of eucommia ulmoids plants can be increased by developing application of extracts of the eucommia ulmoids leaf extracts, the application range of the eucommia ulmoids plants is enlarged, because the eucommia ulmoids leaf is easier to be obtained than eucommia ulmoids skins, the cost of eucommia ulmoids can be reduced.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, and in particular relates to an extract of eucommia leaves and a preparation method and application thereof. Background technique [0002] Eucommia ulmoides is the dry bark of Eucommia ulmoides Oliver, a plant in the family Eucommia, and it is a valuable tonic medicinal material in China. It has many functions such as invigorating liver and kidney, strengthening bones and muscles, lowering blood pressure, and preventing miscarriage. "Shen Nong's Materia Medica" is listed as top grade. It is said that it "cures mainly waist and knee pain, nourishes the middle, nourishes the essence, strengthens the bones, eliminates itching and dampness under the yin, and urinates. Long-term use, light body and old age." Eucommia is a unique medicinal material in China. It has a long history of medicinal use. It has a wide range of clinical applications. So far, as many as 14 species of Eucommia hav...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/46A61P19/08A61K127/00
Inventor 杨中林张欣
Owner CHINA PHARM UNIV
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