Production method of newcastle disease (ND), infectious bronchitis (IB), infectious bursal disease virus (IBD) and viral arthritis (VA) four-joint inactivated vaccine

A technology for bronchitis and bursal disease, applied in antiviral agents, viral antigen components, pharmaceutical formulations, etc., can solve the problems of uncontrollable epidemic development, high death rate, slow growth, etc., and reduce the occurrence of epidemics and spread, rapid antibody production, and long protection period

Inactive Publication Date: 2012-09-26
YEBIO BIOENG OF QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In chickens with acute disease, especially breeder chickens, often due to slow growth or stagnation, weight loss, low feeding utilization rate and high death rate, etc., it brings huge economic losses to the chicken industry.
[0006] There are many varieties of commercialized products on the market at home and abroad, but they are all single traditional vaccines. Multiple use of vaccines, especially inactivated...

Method used

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  • Production method of newcastle disease (ND), infectious bronchitis (IB), infectious bursal disease virus (IBD) and viral arthritis (VA) four-joint inactivated vaccine
  • Production method of newcastle disease (ND), infectious bronchitis (IB), infectious bursal disease virus (IBD) and viral arthritis (VA) four-joint inactivated vaccine
  • Production method of newcastle disease (ND), infectious bronchitis (IB), infectious bursal disease virus (IBD) and viral arthritis (VA) four-joint inactivated vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] (1) Antigen preparation and inspection of semi-finished products:

[0096] 1. Preparation of Chicken Newcastle Disease Virus Liquid

[0097] (1) Inoculate the virus seeds used for production, and use sterilized saline to make 10 -4 Dilute and inoculate 200 10-day-old susceptible chicken embryos, 0.1ml per embryo, seal the pinholes after inoculation, and continue to incubate at 37°C without turning the embryos.

[0098] (2) Incubation and observation After the chicken embryos were inoculated, the embryos were photographed once a day, and 9 chicken embryos that died 60 hours ago were discarded. Thereafter, the embryos were photographed once every 4-6 hours, and the dead embryos were taken out at any time until 96 hours, a total of 152 dead embryos were taken out, and all of them were taken out, with the air chamber upward, and cooled at 2-8°C for 12 hours.

[0099] (3) Harvesting Take out the cooled chicken embryos and harvest the allantoic fluid of the chicken embryos...

Embodiment 2

[0142] Finished product inspection - the laboratory trial-produced a batch of quadruple inactivated vaccine of about 6000ml, the batch number is 2011001.

[0143] 1. character

[0144] Appearance Milky white emulsion.

[0145] Dosage form oil-in-water type. Take a clean straw, suck a small amount of vaccine and drop it into cold water, except for the first drop, it will not spread.

[0146] Stability Draw 10ml of vaccine into a centrifuge tube, centrifuge at 3000r / min for 15 minutes, and the water phase precipitated at the bottom of the tube is 0.1ml, which meets the regulations.

[0147] The viscosity is carried out according to the appendix of the current "Chinese Veterinary Pharmacopoeia", and the viscosity value is 46cP, which meets the regulations.

[0148] 2. The filling volume inspection was carried out according to the method stipulated in the "Chinese Veterinary Pharmacopoeia". The results of the measurement of the three bottles were 251.8ml, 252.2ml, and 252.5ml,...

Embodiment 3

[0166] We conducted a comparative test of the immune effect of the quadruple vaccine and the single vaccine of each component. The test method is as follows:

[0167] With 60 21-day-old SPF chickens, 10 chickens were immunized with quadruple vaccine and single vaccine for Newcastle disease, infectious bronchitis, infectious bursal disease, and viral arthritis. No immunization was used as a control. Breeding in groups. Chickens in each group were blood collected before immunization and 7, 14, 21, 28, 35 days after immunization, serum was separated, and serum antibodies were measured. The results are compared in the table below.

[0168]

[0169] Note:

[0170] Bacterial endotoxin test by gel method

[0171] Pyrosate Endotoxin Rapid Test Kit

[0172] Pyrosate gel endotoxin rapid detection kit is the only gel endotoxin rapid detection reagent in the world, mainly used for the rapid detection of endotoxin in water, lysate, dialysate and other end products.

[0173] 【Ingre...

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Abstract

The invention relates to a production method of a newcastle disease (ND), infectious bronchitis (IB), infectious bursal disease virus (IBD) and viral arthritis (VA) four-joint inactivated vaccine. The production method is characterized by comprising the following steps: adopting ND La Sota strains, IB virus M41 strains, E.coli BL21/pET28a-VP2 strains expressing the IBD virus VP2 protein and VA AV2311 strains as bacterial and viral strains and adopting a superstrong concentration process and high-quality adjuvants to prepare the inactivated vaccine. After being used for immunizing animals, the inactivated vaccine has the characteristics of high antibody production speed, high titer and long period of protection, and can be used for reducing the epidemic disease occurrence and spreading.

Description

technical field [0001] The invention relates to a method for preparing an oil-emulsion vaccine for preventing Newcastle disease, infectious bronchitis, infectious bursal disease and viral arthritis in chickens, and belongs to the field of veterinary biological products. Background technique [0002] Newcastle disease (ND) has a high morbidity and fatality rate. It occurs frequently all over the world. Once it breaks out, it will bring a devastating blow to the poultry industry. It is a major infectious disease that endangers the poultry industry. OIE lists it as a Category A disease. [0003] Chicken infectious bronchitis (Infectious Bronchitis, IB), the disease can lead to slow growth of chicks and broilers, decreased egg production and egg quality of laying hens. The OIE lists it as a Category B disease. [0004] Infectious bursal disease virus (IBD) is a special disease caused by infectious bursal disease virus in the family BiRNAviridae, which damages the bursa of youn...

Claims

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Application Information

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IPC IPC(8): A61K39/295A61P31/14A61K39/12A61K39/15A61K39/17A61K39/215
Inventor 李晓林左青山范根成李陆梅李昌友李芳
Owner YEBIO BIOENG OF QINGDAO
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