Method for cultivating aphelenchoides besseyi by using carrot callus
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A technology of rice stem apical nematode and callus, which is applied in animal husbandry and other fields, can solve the problems affecting plant parasitism and pathogenicity, and achieve the effects of reducing the number of subcultures, mass cultivation and reproduction, and easy acquisition
Inactive Publication Date: 2012-10-03
SOUTH CHINA AGRI UNIV
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The method of cultivating A. sativa with fungi is simple and has a high reproduction rate, but long
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Embodiment 1
[0027] The propagation period of embodiment 1 rice dry sharp nematode on the carrot callus
[0028] See Table 1 below for the populations of the tested rice stem nematodes, all of which are preserved in South China Agricultural University.
[0029] Table 1 Populations and origins of the tested rice stem nematodes
[0030] serial number population number Origin the host 1 HN-2 Luoshan, Henan Province Rice ( Oryza sativa ) 2 N80 Yuanji Village, Yuanji Town, Huaiyin District, Huai'an City, Jiangsu Province Rice ( O. sativa ) 3 N10 Hongxing Village, Xiongzhou Town, Liuhe District, Nanjing City, Jiangsu Province Rice ( O. sativa ) 4 A-1 Huainan Gongji Town, Anhui Province Rice ( O. sativa ) 5 JZ Experimental Field of Yangtze University, Jingzhou, Hubei Province Rice ( O. sativa ) 6 Q2 Xiaozhai Village, Huaxi Town, Huaxi District, Guiyang, Guizhou Province Rice ( O. sativa ) 7 YQ3 Lujiazhai...
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Abstract
The invention discloses a method for cultivating aphelenchoides besseyi by using carrot callus and belongs to the technical field of plant nematodes cultivation and preservation. The method includes specific steps of washing fresh carrots clean, soaking in alcohol, firing unit surfaces of the carrots become dark red, peeling the surfaces, cutting into slices, placing in a sterilization petri dish containing water agar, sealing, placing in an incubator, and cultivating until the carrot callus is grown out; disinfecting the aphelenchoides besseyi by using streptomycin sulfate solution, centrifuging, precipitating, evenly mixing by using sterile water, centrifuging continuously, finally precipitating, diluting by using the sterile water, and dropping on the carrot callus in dispersing mode; and placing the carrot callus with inoculation of the aphelenchoides besseyi in a black incubator, and cultivating at the temperature of 25 DEG C-30 DEG C or preserving at the temperature of 20 DEG C-25 DEG C. The method for cultivating the aphelenchoides besseyi by using the carrot callus can cultivate and reproduce the aphelenchoides besseyi simply and largely and can also maintain parasitic and pathogenic force of the aphelenchoides besseyi to the plant.
Description
technical field [0001] The invention relates to a method for cultivating plant nematodes, in particular to a method for cultivating rice stem nematodes using carrot callus. Background technique [0002] Rice stem-point nematode ( Aphelenchoides besseyi Christie, 1942) disease first appeared in Japan in 1915, and was introduced into China from Japan in the 1940s. basically under control. However, in the past 10 years, the scope of the disease and the severity of the damage have begun to increase again. At present, it is distributed in the main rice areas of my country, and the occurrence and damage are serious in some areas. Rice stem apical nematode is widely distributed, almost all over the rice producing areas, in my country's Guangdong, Guangxi, Hunan, Hubei, Zhejiang, Jiangsu, Anhui, Shandong, Hebei, Shanghai, Liaoning, Shaanxi, Guizhou, Sichuan, Yunnan, Taiwan and other provinces And the main rice areas in the region are distributed. [0003] The host range of rice ...
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