Antioxidant
A technology for compounds and cosmetics, applied in the direction of antitoxin, cosmetics, cosmetic preparations, etc., can solve the problem of not knowing the antioxidant effect and so on
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Embodiment 1
[0039] A C12A7 single crystal produced by the melting zone method (FZ) was processed into a mirror-polished plate with a thickness of 300 μm. It was heat-treated in oxygen at 1350°C for 6 hours (sample A). Then keep it at 1300°C for 2 hours in a mixed gas flow of 20% hydrogen by volume - 80% nitrogen by volume, and then cool rapidly in the same environment to introduce hydride ions into the C12A7 crystal (sample B).
[0040] Next, after heating up to 800° C. in air, cooling was performed to remove or convert hydride ions into hydroxyl groups (OH − ) (sample C). The concentration of hydroxyl groups in each sample was calculated using the intensity of infrared absorption at 3560 cm-1. In addition, the total concentration of hydrogen contained in each sample was quantified by secondary ion mass spectrometry (SIMS).
[0041] In samples A and C, almost all of the hydrogen was used as a proton to form a hydroxyl group. In Sample B, hydrogen exists as hydride ions in addition to p...
Embodiment 2
[0044] DPPH free radical scavenging ability assay
[0045] (1) Preparation of DPPH solution
[0046] DPPH (1,1'-diphenyl-2-picrylhydrazyl, manufactured by Wako Pure Chemical Industries) was dissolved in 100% ethanol to prepare a 0.00394w / v% solution.
[0047] (2) Preparation of sample solution
[0048] Using pure water as a solvent, a 0.1 w / v% dispersion of sample B prepared in Example 1 was prepared. The agglomerates were pulverized by ultrasonic treatment for 5 minutes, and stirred with a stirrer for about 1 hour. Because the powder is easy to settle in pure water, it should be stirred with a stirrer just before mixing with the DPPH solution. The control of the sample solution using pure water as a pure water solvent.
[0049] (3) Determination of ESR spectrum
[0050] (Device ESR-JES-TE200: JEOL)
[0051] Resonant frequency: 9.43GHz, output: 4.0mW, magnetic field modulation: 100kHz, observed magnetic field: 338.5±5mT, measurement time: 1min, modulation amplitude: 0....
Embodiment 3
[0061] About lipid oxidation inhibitory effect (evaluation of antioxidant capacity of squalene and oleic acid by ESR)
[0062] (1) Solvent
[0063] Squalene (purity 99.5% from Kishimoto special liver oil without oxidation inhibitors) and oleic acid (Wako Pure Chemical Industry Grade 1) are used.
[0064] (2) Preparation of spin trapping agent
[0065] The spin trapping agent α-Phenyl-N-tert-butylnitrone (α-phenyl-N-4-butylnitrone) (hereinafter referred to as α-PBN) was dissolved in tri(caprylic / capric) glyceride ( Using ultrasound), make a 400mM solution.
[0066] (3) Measurement sample preparation
[0067] The sample B of Example 1 and the spin-trapping agent (α-PBN) solution were added to the solvent sample and prepared according to the compounding amounts in Table 1 to prepare an ESR measurement sample. The concentration of sample B is the weight percentage calculated by (weight of sample B) / (weight of solvent)×100.
[0068] Table 1
[0069]
[0070] (4) ESR meas...
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