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Tetranuclear diterpenoids as well as preparation and application thereof

A technology of tetracyclic diterpenoids and compounds, applied in the field of tetracyclic diterpenoids, can solve problems such as pollution, serious residues, biological and human health hazards, etc.

Active Publication Date: 2012-10-03
北海南方海洋科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Since the 20th century, the extensive use of chemically synthesized pesticides has brought serious pollution to water bodies and soils. More than 2 / 3 of the pesticides have directly penetrated into the environment, and the residues are very serious, causing direct and potential harm to both organisms and human health.

Method used

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  • Tetranuclear diterpenoids as well as preparation and application thereof
  • Tetranuclear diterpenoids as well as preparation and application thereof
  • Tetranuclear diterpenoids as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The tetracyclic diterpenoids derived from seaweed endophytic fungi are represented by formula (I).

[0023]

Embodiment 2

[0025] The preparation method of the diterpenoid compound as shown in formula (I):

[0026] Take the fungus Trichoderma longibrachiatum (Trichoderma longibrachiatum) cf-11 strain that grows well on the plate, cut into small pieces and inoculate in PDB liquid medium, put 300mL medium in each 1L Erlenmeyer flask, 50 bottles in total, and ferment statically at room temperature On day 35, 1 / 2 volume of ethyl acetate was added to the fermented liquid to kill fungi, filtered, and the mycelia and fermented liquid were collected respectively. The PDB liquid medium consists of 200 milliliters of potato juice per liter, 20 grams of glucose, 5 grams of peptone, 3 grams of yeast extract, 500 milliliters of aged sea water, and 300 milliliters of distilled water. Among them, the fungus Trichoderma longibrachiatum (Trichoderma longibrachiatum) cf-11 strain was preserved in the China Center for Type Culture Collection CCTCC on April 25, 2012, the preservation number is CCTCC M 2012141, the ta...

Embodiment 3

[0035] The difference from Example 2 is that

[0036] Take the fungus Trichoderma longibrachiatum (Trichoderma longibrachiatum) cf-11 strain that grows well on the plate and inoculate it in JY liquid medium, put 300mL medium in each 1L Erlenmeyer flask, 50 bottles in total, ferment statically at room temperature for 30 days, add fermentation Kill fungi with 1 / 2 volume of ethyl acetate, filter, and collect mycelium and fermentation broth respectively. The JY liquid medium consists of 500 milliliters of Jerusalem artichoke tuber juice per liter, 10 grams of glucose, 2.0 grams of sodium nitrate, and 500 milliliters of aged seawater.

[0037] Collect about 15L of fermentation broth, extract three times with ethyl acetate, and concentrate under reduced pressure; after the mycelia is crushed, extract three times with chloroform-ethanol with a volume ratio of 1:1, then extract with ethyl acetate, and concentrate under reduced pressure; The results of layer chromatography detection (pe...

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Abstract

The invention relates to the field of pesticides and bacteriostatic agents, in particular to tetranuclear diterpenoids as well as a preparation and an application thereof, wherein the tetranuclear diterpenoids are sources of seaweed endophytic fungi. The concrete structural formula of the tetranuclear diterpenoids is shown in formula (I), and a preparation method of the tetranuclear diterpenoids comprises the steps as follows: (Trichoderma longibrachiatum)cf-11 is inoculated in fugus fluid nutrient medium for fermentation cultivation; in addition, fermentation products are purified, so as to obtain the tetranuclear diterpenoids shown in formula (I). Pesticidal activity experiments prove that the crustacean lethality is 82.6 percent when the obtained tetranuclear diterpenoids are 100 micrograms per milliliter, and the crustacean lethality is 50 percent when the concentration of the obtained tetranuclear diterpenoids reaches 23.1 micrograms per milliliter; and in addition, the tetranuclear diterpenoids also achieve bacteriostatic activity.

Description

technical field [0001] The invention relates to the field of insecticides and antibacterial agents, in particular to a tetracyclic diterpenoid derived from seaweed endophytic fungi and its preparation and application. Background technique [0002] Since the 20th century, the extensive use of chemically synthesized pesticides has brought serious pollution to water bodies and soils. More than 2 / 3 of the pesticides have directly penetrated into the environment, and the residues are very serious, causing direct and potential harm to organisms and human health. . As people pay more and more attention to food safety, the development and utilization of pollution-free bio-sourced pesticides has gradually been paid attention to. Compared with chemically synthesized pesticides, biological pesticides have the advantages of good environmental compatibility and less resistance. Their development and application are of great significance to human health, environmental protection and sust...

Claims

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Application Information

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IPC IPC(8): C07C49/617C12P7/26A01P7/00A01P1/00C12R1/885
Inventor 季乃云苗凤萍
Owner 北海南方海洋科技开发有限公司
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