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Method for preparing sulphochracein crystals by adopting tremella

A technology for oosporin and Tremella fungus is applied in the field of preparing oosporin crystals and achieves the effects of simple operation, less organic solvent and high content

Inactive Publication Date: 2012-10-03
CHENGDU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far there is no report about preparing oosporin crystals from Tremella fungus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Prepare the fermentation culture of Tremella fungus: Pick the seeds of Tremella fungus and inoculate them in the seed medium, and cultivate them for 5 days at 25°C with a rotation speed of 150r / m to obtain seed liquid, and inoculate the seed liquid with 10% Inoculate a certain amount into the fermentation medium, and cultivate it for 5 days at 25°C and a rotation speed of 150r / min to obtain a Tremella fungus fermentation culture. The seed liquid medium is the same as the fermentation medium, and its composition is: glucose 20g.L -1 +Peptone 5g.L -1 ++ Potassium dihydrogen phosphate 0.46g.L -1 + Dipotassium hydrogen phosphate 1.0g.L -1 +Magnesium sulfate 1.0g.L -1 , pH6.0, the fermented liquid began to turn red after 3 days of fermentation, and the color changed significantly after 12 hours;

[0021] (2) Use 10,000r / min to separate the fermentation broth and mycelium in the fermentation culture, take the fermentation broth, concentrate it under reduced pressure at...

Embodiment 2

[0025] (1) Prepare the fermentation culture of Tremella fungus: Pick the seeds of Tremella fungus and inoculate them in the seed medium, cultivate them for 4 days at 20°C and 200r / min at a rotational speed to obtain seed liquid, and inoculate the seed liquid with an inoculation amount of 8%. Inoculated into the fermentation medium, cultured at 30°C and 150r / min for 7 days to obtain the fermentation culture of Tremella fungus, the seed liquid medium is the same as the fermentation medium, and its composition is: 30g.L sucrose -1 +Peptone 6g.L -1 + corn syrup 4g.L -1 + Potassium dihydrogen phosphate 0.46g.L -1 + Dipotassium hydrogen phosphate 1.0g.L -1 +Magnesium sulfate 1.0g.L -1 , pH6.0, the fermented liquid began to turn red after 5 days of fermentation, and the color changed significantly after 12 hours;

[0026] (2) Separate the fermentation broth and mycelium in the fermentation culture, take the fermentation broth, concentrate it under reduced pressure at 50°C, add 5 ...

Embodiment 3

[0030] (1) Preparation of fermented culture of Tremella fungus: Pick Tremella fungus seeds and inoculate them in the seed medium, culture them at 20°C and 200r / min for 4 days to obtain seed liquid, and inoculate the seed liquid with 5% inoculum Inoculated into the fermentation medium, cultured at 25°C and 150r / min for 8 days to obtain the fermentation culture of Tremella fungus, the seed liquid medium is the same as the fermentation medium, and its composition is: 30g.L sucrose -1 +Beef extract 5g.L -1 +Yeast extract powder 5g.L -1 + soybean meal 5g.L -1 + Potassium dihydrogen phosphate 0.46g.L -1 + Dipotassium hydrogen phosphate 1.0g.L -1 +Magnesium sulfate 1.0g.L -1 , pH6.0, the fermented liquid began to turn red after 5 days of fermentation, and the color changed significantly after 12 hours;

[0031] (2) Separate the fermentation broth and mycelium in the fermentation culture, take the fermentation broth, concentrate it under reduced pressure at 50°C, add 5 times the ...

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PUM

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Abstract

The invention discloses a method for preparing sulphochracein crystals by adopting tremella. The method is realized through the following steps: firstly, selecting an appropriate culture medium for preparing a fermentation culture of the tremella; and then, after adding ethanol into fermentation liquid to remove polysaccharide and proteins, performing low temperature crystallization after absorption and elution by macroporous resin. The method disclosed by the invention has the advantages of short fermentation period, high content of the sulphochracein in the fermentation liquid, simplicity in operation, less organic solvent in use and the like.

Description

technical field [0001] The invention relates to a method for preparing oosporin crystals, in particular to a method for preparing oosporin crystals by adopting tremella fungus. Background technique [0002] Oosporins are metabolites of many soil fungi, entomogenic fungi and basidiomycetes. According to literature reports, oosporin has a wide range of biological activities and is mainly used for biological control. In 1984, D Brewer reported that oosporin has antibacterial effect. In 1992, BJ Terry reported that oosporin can inhibit type I herpes simplex virus. The activity of DNA polymerase, J Eyal reported in 1994 that oosporin has a strong anti-pest effect such as plant leaf whitefly, soil insect weevil, etc. Hermann Strasser research in 2000 showed that oosporin has no effect on humans and animals. Toxic effect, Hu Fenglin reported in 2006 that oosporin has the activity of inhibiting monoamine oxidase (MAO). In view of the wide range of biological activities of oosporin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/26C12R1/645
Inventor 苟小军陈封政颜军何钢刘嵬
Owner CHENGDU UNIV