Dual hyper-immune egg yolk antibody injection for duck virus hepatitis and duck plague and preparation method thereof

Abstract

The invention relates to a dual hyper-immune egg yolk antibody injection for duck virus hepatitis and duck plague and a preparation method of the injection. The preparation method comprises the following steps: separating strains to prepare a vaccine, immunizing healthy ducks to obtain eggs, and performing disinfection, acidification, octanoic acid treatment, refining extraction and purification, preparation and packaging on the eggs to obtain the dual hyper-immune egg yolk antibody injection for duck virus hepatitis and duck plague. The preparation method in the application has the characteristics that: the vaccine is prepared by firstly sampling and separating the viruses in a disease prevalence area and screening the virus strains wider in coverage; then hot and cold acidification treatments are carried out to obtain a clear crude egg yolk antibody solution, and this technology is not mentioned in the existing production; and then the egg yolk antibody solution is obtained after octanoic acid treatment, low-temperature high-speed centrifuge, microfiltration and ultrafiltration. The dual egg yolk antibody injection prepared by the method not only is different from those produced by other companies in preparation types, but also has other advantageous features in the production process such as low loss, high purity and high biological activity, and no clinical side effects are brought about.

Description

technical field [0001] The invention belongs to the technical field of biological products for poultry, and relates to a duck viral hepatitis and duck plague dual high immunity yolk antibody injection, and also relates to the duck viral hepatitis and duck plague dual high immunity egg yolk antibody injection method of preparation. Background technique [0002] Duck viral hepatitis, referred to as duck hepatitis, is one of the most serious diseases that harm ducklings. It mainly harms ducklings less than 20 days old. The younger the ducklings, the higher the mortality rate, and the highest mortality rate can reach 90%-95. %, and all antibiotics are ineffective against this disease. Duck viral hepatitis was discovered in my country as early as 1958, and the disease became prevalent in my country again in the early 1980s. Since then, epidemics have occurred one after another in various places, and the epidemic can be controlled by the immunization of duck viral hepatitis I typ...

AI Technical Summary

Problems solved by technology

In addition, in actual production, animal blood collection is not only a great stress on the animal itself, but also time-consuming and labor-intensive. Large-scale production is impractical, and it is much more convenient to use eggs to produce antibodies.

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