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Method for concurrently extracting 1-deoxynojirimycin and resveratrol from plant

A technology of resveratrol and plants, applied in the field of rapid extraction and separation of 1-deoxynojirimycin, simultaneous extraction and separation of 1-deoxynojirimycin, resveratrol, capable of resolving 1-deoxynojirimycin and Bailey Problems such as easy loss of revertyl alcohol, high production conditions, and long flocculation and sedimentation time, etc., to achieve the effects of guaranteed follow-up resources, simplified production process, and easy planting

Inactive Publication Date: 2012-10-17
长沙湘资生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since 1-deoxynojirimycin and resveratrol are mainly used in the pharmaceutical and food industries, the 1-deoxynojirimycin and resveratrol products extracted by these patented methods are harmful to human health; in addition, although in Chinese patent 200910192542.2 There is no introduction of toxic reagents for separation and purification, and the use of flocculants for flocculation and precipitation takes a long time, and 1-deoxynojirimycin and resveratrol are easily lost during this process, which is not conducive to improving their yields. Furthermore, the process used Complicated, demanding production conditions, low yield, not conducive to reducing production costs

Method used

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  • Method for concurrently extracting 1-deoxynojirimycin and resveratrol from plant

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Crush and dry 100kg of mulberry leaves, add 200L of water, add 10kg of yeast As2.502, keep warm at 30°C for a certain period of time for 60h, heat and reflux at 80°C for 1h, and filter the extract twice through three layers of gauze and ceramic membrane. Concentrate under pressure to 30L, add 5kg granular activated carbon for decolorization for 20min, after decolorization, the solution is separated with LSA-10 macroporous adsorption resin, first use pure water to elute impurities, then use 50% ethanol to elute, and collect the ethanol eluate , concentrated under reduced pressure to 50L, slowly lowered the temperature to room temperature to crystallize for 24h, and obtained 214g DNJ samples.

[0037] The residue after water extraction of mulberry leaves was extracted with 550L of 50% ethanol, heated and refluxed at 80°C for 2h, filtered twice with three layers of gauze, filtered with ceramic membrane, concentrated under reduced pressure to 70L (no ethanol smell), and then...

Embodiment 2

[0040] Crush and dry 400kg of mulberry leaves, add 200L of water, add 10kg of yeast As2.502, incubate at 30°C for a certain period of time for 60h, heat and reflux at 80°C for extraction for 1h, filter the extract twice through three layers of gauze, and filter through a ceramic membrane. Concentrate under pressure to 30L, add 15kg of granular activated carbon to decolorize for 20min, and use LSA-10 macroporous adsorption resin to separate the decolorized solution, first use pure water to elute impurities, then use 50% ethanol to elute, and collect ethanol eluate , concentrated under reduced pressure to 150L, ​​slowly lowered the temperature to room temperature to crystallize for 24h, and obtained 837g DNJ sample.

[0041] The residue after water extraction of mulberry leaves was extracted with 2700L of 50% ethanol, heated and refluxed at 80°C for 2h, filtered twice with three layers of gauze, filtered with a ceramic membrane, concentrated under reduced pressure to 100L (no eth...

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Abstract

The present invention relates to a method for concurrently extracting 1-deoxynojirimycin and resveratrol from mulberry leaves, wherein yields and purities of the 1-deoxynojirimycin and the resveratrol are substantially improved with the method. According to the present invention, a complete method for separating and extracting high purity 1-deoxynojirimycin and high purity resveratrol from mulberry leaves is provided; after microbial conversion, alcohol reflux extraction, membrane filtration, concentration, extraction, and active carbon decoloration are performed, conditions of crystallization and recrystallization are controlled to respectively obtain 1-deoxynojirimycin and resveratrol; requirements on environmental conditions and equipment are not high during the whole separation process, the separation time is shortest, the purity is highest, and the comprehensive utilization rate of raw materials is highest; the separated material is easy to obtain and cheap, the separation process is simple and easy to control, crystallization and recrystallization are adopted, the purification efficiency is high, the used reagent is nontoxic and safe, and the cost is low.

Description

Technical field: [0001] The invention relates to a process technology for simultaneously extracting and separating 1-deoxynojirimycin and resveratrol, in particular to a process technology for rapidly extracting and separating 1-deoxynojirimycin and resveratrol from mulberry leaves, belonging to The technical field of bioseparation engineering. Background technique [0002] my country's mulberry resources and output rank first in the world, and are cultivated all over the country. At present, mulberry is cultivated as forage in most areas. After winter, the breeding of winter silkworms decreases, and a large amount of mulberry leaves are discarded. Mulberry leaves taste bitter, sweet, cold in nature, and return to the lung and liver meridian. 1-deoxynojirimycin (DNJ), resveratrol and other components in leaves, so it is of great significance to study the extraction technology of these two components in mulberry leaves. [0003] At the same time, after research, resveratro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D211/46C07C39/21C07C37/70
Inventor 唐忠海
Owner 长沙湘资生物科技有限公司