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Hand-operated air pressure sample introduction method for microfluidic chips

A microfluidic chip and air pressure technology, applied in the field of analytical chemistry, can solve problems such as insufficient, poor controllability, and difficult on-site detection, and achieve the effect of simple equipment structure, high cost performance, and reliable performance

Inactive Publication Date: 2012-10-17
INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are currently existing manual simple sampling methods: negative pressure, static pressure, air pump, peristaltic pump, etc., the design concept innovation is limited, the controllability level is not enough, the sampling performance is not enough for practical requirements, and it is difficult to be used outside the laboratory. on-site testing

Method used

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  • Hand-operated air pressure sample introduction method for microfluidic chips
  • Hand-operated air pressure sample introduction method for microfluidic chips
  • Hand-operated air pressure sample introduction method for microfluidic chips

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Such as image 3 As shown, the sample injection method of the present invention is specifically implemented as follows:

[0063] 1. In C 4 Steps for use on the microfluidic chip:

[0064] ① if image 3 Use a pipette to drop various trace liquids required for the reaction through the sample injection 12 (also a pressure port), and drop them into the built-in cavity (ie, pre-reaction and mixing area) on the microfluidic chip card. Perform a pre-stay reaction, wait for pressurization at any time, and move to the direction of the waste liquid outlet.

[0065] ② if Figure 4 , twitch the syringe plunger, use the outlet of the syringe joint to apply air pressure, insert it into the injection port 12 on the chip, and anastomose to form a seal. Push the piston to form an air pressure P. Under the action of P, the sample liquid in the cavity slowly moves to the right, as indicated by the arrow, passes through the detection electrode, and flows to the waste liquid outlet.

...

Embodiment 2

[0068] Due to the microfluidic chip manual air pressure sampling technology of the present invention, it can be widely used in combination with various detection devices (detectors). Therefore, for specific operation and use, the sampling technique of the present invention is basically irrelevant to what kind of detector and detection device is used.

[0069] Such as Figure 5 , is an example of the application of the design principle of the present invention (manual air pressure to drive liquid flow) on the MCE (Microchip Capillary Electrophoresis, chip capillary electrophoresis) microfluidic chip. The usage steps are basically similar to the above 1.

[0070] Such as Figure 5, in the design of the chip card, the buffer driving electrode in the conventional MCE microfluidic chip is canceled, and one separation electrode 20 is set on the inner left wall of the buffer mixing area, and the other is set on the waste liquid outlet 23. During detection, a slightly excess buffer...

Embodiment 3

[0072] Using the microfluidic chip manual mixing simple pressure sampling method of the present invention, the K in the water sample has been successfully implemented + , Mg 2+ Ion separation from background buffer ethanesulfonic acid (MES)-histidine (His), and C 4 D detection.

[0073] The detection principle is to use background buffer ethanesulfonic acid (MES)-histidine (His) to separate and separate and detect K + , Mg 2+ Ions, driven by the air pressure of the injector, flow slowly (about 1mm / s) through such as figure 2 When the conductance detection electrode is 15, the measured continuous current change waveform features, and the K in the liquid sample is measured + , Mg 2+ The presence of characteristic conductance signals.

[0074] Device conditions for the detection test: MES:His ratio 1:0.9, pH value 4 D excitation signal is continuous sine wave 100Hz, voltage 5V, completed by one DG1022 general source signal generator. Such as figure 2 One piece of microf...

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Abstract

The invention belongs to a hand-operated air pressure sample introduction method for microfluidic chips. According to a hand-operated mixed sample introduction detection method for microfluidic chips, pressure generated by pushing of a section of enclosed air to another end is used as power to drive liquid in a chip detecting micro-channel circuit which forms an enclosed passage to flow along a designated target direction and to pass the position of a detector on a microfluidic chip so as to complete analysis and detection of substances; a cavity 11 is provided at a sample introduction inlet of the microfluidic chip for storage of a reaction solution and air used as a driving means, which is beneficial for people to control reaction and detection timing, and continuous air pressure is easily generated and is used as power needed in flowing of the liquid in the microfluidic chip, which enables driving to be realized. The hand-operated mixed sample introduction method provided in the invention has high cost performance and is simple and practical; the method is an optimal lightweight sample introduction scheme and is especially applicable to on-site detection and analysis in the field. The method can be widely applied to a variety of microfluidic chip detection apparatuses and has good versatility.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and relates to a micro-total analysis system used in microfluidic chip detection technology, in particular to a microfluidic chip manual air pressure sampling method. Background technique [0002] Under the current environmental situation and people's strong awareness of environmental protection, real-time, on-line, green microanalysis technology has become the main development trend of analytical chemistry. Various micro-analysis techniques have emerged, and micro-total analysis technology has become one of the most eye-catching new technical fields. The main purpose of the Miniaturized Total Analysis Systems technology is to integrate the basic operating units of analytical chemistry such as sample preparation, reaction, separation, and detection in the biological, chemical, and medical analysis processes into a micro-sized chip. It can achieve micro-sampling and automatically complete the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N35/10G01N27/00
Inventor 马新华刘楠李晓丽欧国荣
Owner INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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