Production method for extracting active substances from beer lees

A production method and technology of active substances, applied in peptide preparation methods, chemical instruments and methods, animal feed, etc., can solve the problems of waste of active substances, low extraction rate, no further utilization of vinasse residue, etc., and meet the technological conditions mild effect

Inactive Publication Date: 2012-10-24
CHANGCHUN VOCATIONAL INST OF TECH
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  • Abstract
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Problems solved by technology

[0003] Literature and patents also have some reports on the deep processing of brewer's grains, but the way to use them is relatively simple. Some of them are made into animal feed after simple treatment, such as extracting protein with hydrolytic enzymes. After the protein is simply obtained, other active ingredients are discarded. Utilization rate and economic value are all low, so that many active substances in brewer's grains are wasted
Some use distiller's grains to produce dietary fiber, feed oil, and protein powder at the same time, but the method is complicated, the extraction rate is not high, and the distiller's grain residue after extraction is not further utilized

Method used

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  • Production method for extracting active substances from beer lees

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Embodiment 1

[0013] After the wet distiller's grains are dried and crushed, accurately weigh 100 kg of distiller's grains powder, add 450 liters of ethanol solution with a concentration of 95% according to the ratio of solid to liquid ratio of 1:4.5, and extract the pigment in an ultrasonic cell pulverizer for 70 minutes. , ultrasonic power 744.00 kilowatts, centrifugation 4000 rpm, supernatant concentrated under reduced pressure to obtain 3.5 kg of pigment. Add the alcohol-alkali (1:3) solution to the lower layer of precipitate to extract the protein at 1:50 (Kg:l), stir, extract for 90 minutes, centrifuge for 15 minutes (4000 rpm), adjust the supernatant to pH 4.7 and then centrifuge 12 minutes (5000 rpm / min), 10.2 kilograms of protein was obtained, and the lower layer was precipitated to extract dietary fiber by alkali method, and 150 liters of sodium hydroxide (0.9%) was added according to a solid-liquid ratio of 1:17 (Kg:l), and heated in a water bath The temperature is 70°C, and the ...

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Abstract

Belonging to the technical field of food processing, the invention provides a production method for extracting active substances from beer lees. The method comprises: 1. adding ethanol into distiller dried lees, with the lees and the ethanol being in a ratio of 1:3-1:10, controlling an ultrasonic time at 50-120min, and performing centrifugation at a speed of 5000r / min so as to obtain a pigment of the beer lees; 2. adding ethanol and sodium hydroxide solutions into the remaining solid of the lees, with ethanol and the sodium hydroxide being in a ratio of 1:1-1:5, and controlling the lees and the ethanol-sodium hydroxide solution in a ratio of 1:40-1:80, conducting extraction for 60-120min, performing centrifugation at a speed of 5000r / min, adjusting the pH to 4.7, carrying out centrifugation at a speed of 5000r / min, thus obtaining protein of the beer lees; and 3. adding a sodium hydroxide solution into the remaining solid of the lees, controlling the solid of the lees and the sodium hydroxide solution in a ratio of 1:15-1:20, conducting heating to a temperature of 50-80DEG C, then performing stirring and extraction, carrying out centrifugation at a speed of 5000r / min, thus obtaining the dietary fiber of the beer lees. The method of the invention has the beneficial effects that: (1) integrated comprehensive utilization of beer lees is realized, specifically from pigment extraction, protein separation, dietary fiber, and finally to feed application; and (2) the method is characterized by environmental protection and energy saving, joint application of combined techniques, and mild technological conditions.

Description

technical field [0001] The invention belongs to the technical field of food processing. Background technique [0002] In recent years, my country's beer industry has developed rapidly and has become the world's largest beer producer. The annual beer production is 7×107t, and the brewer's grains produced can reach 10×106t. However, most of the processing of beer grains in my country's beer enterprises is to discharge them directly, and some of them are sold cheaply to farmers as roughage. Brewer's grains contain a lot of active substances such as dietary fiber and protein, and are a good source of dietary fiber and protein. How to turn waste into wealth, rationally utilize brewer's grains, make it produce economic benefits, and effectively solve the problem of environmental pollution and waste of resources has become a major issue facing the beer industry. [0003] Literature and patents also have some reports on the deep processing of brewer's grains, but the way to use th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00C07K1/14A23L1/308A23K1/06A23K10/38
CPCY02P60/87
Inventor 逯家富姜福佳徐亚杰翁连海刘黎红周毓麟王宇梁佳峰王丽
Owner CHANGCHUN VOCATIONAL INST OF TECH
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