Construction method of fin cell line of anabarilius grahami

A construction method, the technology of the white fish of the scorpion, applied in the field of the construction of the fin cell line of the white fish of the stingray, can solve the problems such as the same report of the same invention that has not been seen, and achieve the effect of good cell growth and easy operation

Active Publication Date: 2012-10-31
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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Problems solved by technology

[0004] Through document retrieval, do not see identical report with the present invention

Method used

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Embodiment Construction

[0018] The method for constructing the fin cell line of the white fish of the present invention, the specific steps are as follows:

[0019] 1. Preparation of cell culture medium

[0020] Select DMEM / F12 medium, add fetal bovine serum and human basic fibroblast growth factor to the medium, so that the final concentration of fetal bovine serum is 20%, and the concentration of human basic fibroblast growth factor is 5 ng / ml, with a pH value of 7.0-7.4, stored in a refrigerator at 4°C, for later use;

[0021] 2. Primary culture

[0022] First, soak the fresh and live whitefish with 8 mg / L potassium permanganate for 10 min, then disinfect the fish as a whole, and take the fin rays of whitefish on the ultra-clean bench; Wash with HBSS solution of penicillin at a concentration of 100 IU / ml, streptomycin at a concentration of 100 μg / ml, and amphotericin B at a concentration of 10 μg / ml, then cut to ±1 mm 3 The small pieces of fin ray tissue were digested with 0.5% hyaluronidase a...

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Abstract

The invention relates to a construction method of a fin cell line of anabarilius grahami and belongs to the technical field of freshwater aquatic organism cell culture. The method comprises that the anabarilius grahami tail fin tissue cell serves as a material, a tissue block method is adopted, and the primary culture is strated, culturing is achieved in a dulbecco modified eagle medium (DMEM)/F12 containing fetal calf serum and human basic fibroblast growth factors and having a PH value ranging between 7.0 and 7.4; and subculture is performed by using a trypsin digestion method. The method has the advantages that 1, the minimally invasive processing is adopted, on the premise that the fish is alive, and the operation is easy and feasible; 2, the anabarilius grahami fin muscle cell line (AGF) is in a fibroblast cell patternin a fiber structure, continuous passage can be achieved, the line can be applied to biological characteristic research directly, and requirement for anabarilius grahami molecular cell level theoretical research is met; and 3, the construction method is also applicable to cell lines of constructed fin rays of other fishes.

Description

technical field [0001] The invention relates to a method for constructing a fin cell line of stingray white fish, and belongs to the technical field of freshwater aquatic organism cell culture. Background technique [0002] Anabarilius grahami belongs to Cypriniforms, Cyprinidae, and Anabarilius, commonly known as white fish. It is a rare fish species only distributed in Fuxian Lake, Yunnan, and it is one of the four famous fish in Yunnan. The fish is tender and delicious, with soft spines and thin scales, and a delicious fragrance. It is the main fishery target of fishermen along the lake. Historically, its output has accounted for 70%-80% of the total fishery output of Fuxian Lake. In the past ten years, due to the invasion of alien fish species, the deterioration of water quality and overfishing, it has been on the verge of extinction. Although since 1999, the Kunming Institute of Zoology, Chinese Academy of Sciences has successfully bred the whitefish artificially and e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12R1/91
Inventor 王晓爱杨君兴潘晓赋陈小勇
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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