Preparation and application of special mildew preventing agent for bamboo wood and bamboo products

A technology of antifungal agents and bamboo products, applied in the fields of application, fungicides, chemicals for biological control, etc., can solve the problems of unsatisfactory antibacterial effect, limited source of raw materials, high production cost, etc., and achieve low production cost and source The effect of convenience and broad application prospects

Active Publication Date: 2014-02-19
福州科力恩生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to reasons such as unsatisfactory antibacterial effect, high production cost, limited source of raw materials and low output, it has not been popularized and applied.

Method used

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  • Preparation and application of special mildew preventing agent for bamboo wood and bamboo products
  • Preparation and application of special mildew preventing agent for bamboo wood and bamboo products
  • Preparation and application of special mildew preventing agent for bamboo wood and bamboo products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] 1. Activate the C3 strain of Lysobacterium enzymogenes.

[0045] The enzyme-producing lysobacterium ( Lysobacter enzymogenes ) The C3 strain was inoculated in the pre-prepared test tube slant medium, and cultured at 24°C for 52h.

[0046] The formula of the slant medium is peptone: 10g, yeast extract: 5g, sodium chloride: 10g, agar: 20g, tap water: 1L; the pH of the medium is 7.0-7.2, and then sterilized at 121°C for 20min.

[0047] The Lysobacter zymogenes C3 strain comes from the American Type Culture Collection, and the strain number is ATCC29487.

[0048] 2. Preparation of shake flask seed solution

[0049] Pick the activated Lysobacterium enzymogenes C3 strain from the slant in the previous step, transfer it to 4 conical flasks containing 100ml of culture solution, and culture it at 28°C and 230rpm for 40h to prepare the shake flask seed solution. One loop of activated Lysobacterium lysobacterium C3 strain was transferred to each conical flask.

[0050] The fo...

Embodiment 2

[0058] 1. Activate the C3 strain of Lysobacterium enzymogenes.

[0059] Inoculate the Lysobacterium enzymogenes C3 strain stored at -80°C in the pre-prepared test tube slant medium on a sterile workbench, and culture at 30°C for 36 hours.

[0060] The formula of the slant culture medium is the same as in Example 1.

[0061] 2. Preparation of shake flask seed solution

[0062] The activated Lysobacterium enzymogenes C3 strain was picked from the slope, transferred to 7 conical flasks containing 100ml of culture solution, and cultured at 24°C and 230rpm for 48h to prepare shake flask seed solution. One loop of activated Lysobacterium lysobacterium C3 strain was transferred to each conical flask.

[0063] The formulation of the culture solution and the sterilization method are the same as in Example 1.

[0064] 3. Preparation of Lysobacterium enzymogenes C3 antifungal agent

[0065] Using a 10 L fermenter, inject 6L of culture fluid, inoculate 600ml shake flask seed liquid in...

Embodiment 3

[0073] 1. Activate the C3 strain of Lysobacterium enzymogenes.

[0074] Same as Example 1.

[0075] 2. Preparation of shake flask seed solution

[0076] Same as Example 1.

[0077] 3. Preparation of Lysobacterium enzymogenes C3 antifungal agent

[0078] Same as Example 2.

[0079] 4. Antifungal agent heat sterilization treatment

[0080] The preparation process is the same as that of heat treatment example 2, and the prepared antifungal agent is stored at room temperature for 180 days before use.

[0081] 5. Anti-mildew treatment of bamboo soaking method

[0082] Cut the fresh bamboo into small pieces of about 7×0.5cm, and divide them into two groups at random. The control group was soaked in sterile water for 4 hours, and the treatment group was soaked in 180-day stored lysobacterium C3 antifungal agent for 4 hours. After soaking, take them out and place them in dry sterile petri dishes, and place them at room temperature. The result is as Figure 5 As shown, the smal...

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Abstract

The invention relates to a preparation and an application of a special mildew preventing agent for bamboo wood and bamboo products. The preparation process comprises the following steps of: using a slant culture medium to activate and culture a lysobacter enzymogenes C3 bacterial strain; transferring the bacterial strain to seed culture liquid; carrying out culturing for 48h at 24-30 DEG C and 230rpm; transferring the bacterial strain to fermentation culture liquid in 10% proportion; and culturing to obtain the mildew preventing agent. The mildew preventing agent can resist a heat treatment for 1h at 70 DEG C or can be stored for more than 180 days without a remarkable activity influence; after the mildew preventing agent treats the bamboo wood in three methods, namely soaking (for more than 4h), uniformly spraying or daubing, a mildew preventing effect is very remarkable; the bamboo wood keeps an original color andperformance. The invention provides a high-efficient biological mildew preventing agent and the application of mildew prevention of the bamboo wood; production cost is low; the conventional microbial fermentation equipment and a technology can be utilized for mass production; and source is very convenient. Mildew-preventing and preservative effects of the bamboo wood is very remarkable by different treatment methods, therefore, the mildew preventing agent has a wide application prospect in preventing mildew and corrosion of the bamboo products.

Description

technical field [0001] The invention relates to the technical field of biological mildew prevention, in particular to the preparation and application of a special microbial anti-mold agent for bamboo and bamboo products. Background technique [0002] my country is a country that is short of forest resources, and it is an urgent task to find new wood substitute resources. The chemical composition of bamboo is similar to that of wood, mainly composed of cellulose, hemicellulose and lignin, which can replace wood. Moreover, my country's bamboo resources are also very rich, with a total of 39 genera and more than 500 species. Bamboo also has the advantages of rapid growth, high yield, early maturity, and wide application. Expanding the development and utilization of bamboo is of great significance to local economic development and ecological construction. However, bamboo contains more starch, polysaccharides and water-soluble nutrients than wood. Among them, the protein conte...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01P3/00C12N1/20B27K5/00B27K3/52C12R1/01
Inventor 王明兹陈志红沈月毛杜良成陈必链黄建忠
Owner 福州科力恩生物科技有限公司
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