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Heterodimer Core-shell Nanoparticle In Which Raman-active Molecules Are Located At A Binding Portion Of A Nanoparticle Heterodimer, Use Thereof, And Method For Preparing Same

A nanoparticle, Raman active technology, applied in the field of nanoparticle dimer, can solve the problems of nanoparticle instability, unresolved, inhomogeneous, etc.

Active Publication Date: 2012-12-05
KOREA RES INST OF CHEM TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Thus, while the enhanced sensitivity of Raman detection is apparently not uniform within colloidal particle aggregates, it varies with the presence or absence of hot spots
However, the physical structure of such hotspots, the range of distances between nanoparticles to achieve enhanced sensitivity, and the spatial relationship between analyte and aggregate nanoparticles for enhanced sensitivity have not yet been addressed.
Furthermore, aggregated nanoparticles are inherently unstable in solution and thus counterproductive to the reproducibility of single-molecule analyte detection
[0011] In addition, although theoretical simulations and proof-of-concepts of the dimer structures of gold or silver have been attempted, there has not been a precedent for the actual preparation of placing single molecules at the junction between nanoparticles.
Synthesis of gold or silver nanoparticles into well-defined reproducible structures with SERS remains a challenging technical topic

Method used

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  • Heterodimer Core-shell Nanoparticle In Which Raman-active Molecules Are Located At A Binding Portion Of A Nanoparticle Heterodimer, Use Thereof, And Method For Preparing Same
  • Heterodimer Core-shell Nanoparticle In Which Raman-active Molecules Are Located At A Binding Portion Of A Nanoparticle Heterodimer, Use Thereof, And Method For Preparing Same
  • Heterodimer Core-shell Nanoparticle In Which Raman-active Molecules Are Located At A Binding Portion Of A Nanoparticle Heterodimer, Use Thereof, And Method For Preparing Same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] Example 1: Preparation of gold-silver core-shell nanoparticles with Raman active molecules (Cy3) located at the junction of two nanoparticles

[0087] The Raman-active gold-silver core-shell dimer was synthesized based on the DNA-directed bridging method (DNA-directed bridging method) that binds target oligonucleotides to gold nanoparticles and further adjusts the silver shell according to the amount of silver precursor (Figure 1 ).

[0088] First, a highly purified gold nanoparticle dimer structure with target oligonucleotides is obtained by precisely controlling the molar ratio of protecting oligonucleotide sequences and target capture oligonucleotide sequences and purifying them efficiently . Since the maximum distance (d; gap distance) between the surface of gold nanoparticles (AuNP) and Cy3 molecules remains at 7.5nm, it is necessary to reduce the distance in order to obtain amplified electromagnetic enhancement. Because the enhancement effect of silver is severa...

Embodiment 2

[0092] Example 2: UV-visible spectroscopy (UV-visible spectroscopy) and HR-TEM image analysis

[0093] The formation of gold nanoparticle dimers (Cy3 was used as Raman active molecule) was confirmed by UV-visible spectroscopy and high-resolution transmission electron microscopy (HRTEM) images (Fig. 2). The UV-Vis spectrum showed some red shift after dimer formation, which is consistent with the results previously reported by Oaul Alivisatos et al. (Angew chem. 1999.38(12), 1808). Figure 2a A typical HR-TEM image of a gold nanoparticle dimer is shown. The analysis results of a minimum of 200 particles found that 25% of the particles were monomers, 65% of the particles were dimers, and less than 10% of the particles were trimers or tetramers or higher polymers. According to HR-TEM analysis, the particle distance between gold particles is about 2~3nm. It is expected that the inter-nanoparticle spacing in the solution state (0.3PBS) is actually longer than that in the dry state...

Embodiment 3

[0095] Example 3: AFM (Atomic Force Micrograph) Analysis of Gold-Silver Core-Shell Nanoparticles

[0096] Figure 3aEnlarged AMF (atomic force micrograph) image (1 μm × 1 μm) showing typical core-shell monomer and heterodimer nanostructures (Cy3 used as Raman active molecule). Its shape and diameter are consistent with the results of HR-TEM analysis. Figure 3b show right Figure 3a AFM images of single particles were rectified for SERS spectra. Because there is no hot spot in the core-shell monomer structure and only one Cy3 molecule exists, so for a silver shell thickness of 5nm ( Figure 3a -1) or 10nm ( Figure 3a -2) No SERS signal was detected for the gold-silver core-shell monomer. No SERS signal was also detected for gold dimers without silver shells or gold dimers with spacing below 1 nm. This is because the electromagnetic enhancement under the excitation condition of 514.5nm laser is insufficient. Thin shell thickness (less than 3nm) Figure 3a In the case of -4,...

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Abstract

The present invention relates to a nanoparticle heterodimer in which Raman-active molecules are located at a binding portion of the nanoparticle heterodimer, and more particularly, to a core-shell nanoparticle heterodimer comprising: a gold or silver core having a surface to which oligonucleotides are bonded; and a gold or silver shell covering the core. In addition, the present invention relates to the core-shell nanoparticle heterodimer, to a method for preparing same, and to the use thereof.

Description

technical field [0001] The present invention relates to a nanoparticle dimer, which is prepared so that the Raman active molecule is located at the junction of the dimer composed of nanoparticles, more specifically, relates to a core-shell nanoparticle dimer, on the surface of the core Oligonucleotides are bound and formed by a gold or silver shell surrounding the core. The nanoparticles forming the core are gold nanoparticles or silver nanoparticles. The present invention also relates to the core-shell nanoparticle dimer, its preparation method and application. Background technique [0002] Highly sensitive and accurate detection of single molecules from biological and other samples can find broad applications in medical diagnostics, pathology, toxicology, environmental sampling, chemical analysis, and many other fields. For this reason, in the past few years, nanoparticles and chemical substances labeled with specific substances have been widely used in the field of bio-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B82B1/00B82B3/00G01N33/53C12Q1/68
CPCG01N33/54346C12Q1/6816C12Q1/6834G01N33/54373C12Q2563/155C12Q2563/107C12Q2565/628C12Q2565/632C12Q2563/143C12Q2537/125C12Q1/6827G01N33/5302
Inventor 徐宁德南佐旻林东权全基锡
Owner KOREA RES INST OF CHEM TECH
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