Heterodimer Core-shell Nanoparticle In Which Raman-active Molecules Are Located At A Binding Portion Of A Nanoparticle Heterodimer, Use Thereof, And Method For Preparing Same
A nanoparticle, Raman active technology, applied in the field of nanoparticle dimer, can solve the problems of nanoparticle instability, unresolved, inhomogeneous, etc.
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Embodiment 1
[0086] Example 1: Preparation of gold-silver core-shell nanoparticles with Raman active molecules (Cy3) located at the junction of two nanoparticles
[0087] The Raman-active gold-silver core-shell dimer was synthesized based on the DNA-directed bridging method (DNA-directed bridging method) that binds target oligonucleotides to gold nanoparticles and further adjusts the silver shell according to the amount of silver precursor (Figure 1 ).
[0088] First, a highly purified gold nanoparticle dimer structure with target oligonucleotides is obtained by precisely controlling the molar ratio of protecting oligonucleotide sequences and target capture oligonucleotide sequences and purifying them efficiently . Since the maximum distance (d; gap distance) between the surface of gold nanoparticles (AuNP) and Cy3 molecules remains at 7.5nm, it is necessary to reduce the distance in order to obtain amplified electromagnetic enhancement. Because the enhancement effect of silver is severa...
Embodiment 2
[0092] Example 2: UV-visible spectroscopy (UV-visible spectroscopy) and HR-TEM image analysis
[0093] The formation of gold nanoparticle dimers (Cy3 was used as Raman active molecule) was confirmed by UV-visible spectroscopy and high-resolution transmission electron microscopy (HRTEM) images (Fig. 2). The UV-Vis spectrum showed some red shift after dimer formation, which is consistent with the results previously reported by Oaul Alivisatos et al. (Angew chem. 1999.38(12), 1808). Figure 2a A typical HR-TEM image of a gold nanoparticle dimer is shown. The analysis results of a minimum of 200 particles found that 25% of the particles were monomers, 65% of the particles were dimers, and less than 10% of the particles were trimers or tetramers or higher polymers. According to HR-TEM analysis, the particle distance between gold particles is about 2~3nm. It is expected that the inter-nanoparticle spacing in the solution state (0.3PBS) is actually longer than that in the dry state...
Embodiment 3
[0095] Example 3: AFM (Atomic Force Micrograph) Analysis of Gold-Silver Core-Shell Nanoparticles
[0096] Figure 3aEnlarged AMF (atomic force micrograph) image (1 μm × 1 μm) showing typical core-shell monomer and heterodimer nanostructures (Cy3 used as Raman active molecule). Its shape and diameter are consistent with the results of HR-TEM analysis. Figure 3b show right Figure 3a AFM images of single particles were rectified for SERS spectra. Because there is no hot spot in the core-shell monomer structure and only one Cy3 molecule exists, so for a silver shell thickness of 5nm ( Figure 3a -1) or 10nm ( Figure 3a -2) No SERS signal was detected for the gold-silver core-shell monomer. No SERS signal was also detected for gold dimers without silver shells or gold dimers with spacing below 1 nm. This is because the electromagnetic enhancement under the excitation condition of 514.5nm laser is insufficient. Thin shell thickness (less than 3nm) Figure 3a In the case of -4,...
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