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Substrate for ligand immobilization and method for producing same

A manufacturing method and technology of ligands, applied in chemical instruments and methods, other chemical processes, blood diseases, etc., can solve problems such as small interactions, and achieve the effect of high adsorption performance

Active Publication Date: 2012-12-26
ASAHI KASEI MEDICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported that a copolymer of CMB and a polymerizable monomer has excellent biocompatibility such as low interaction with biological components such as proteins and blood cells (Patent Document 6), but the surface of the substrate has CMB and a functional group for ligand immobilization The substrate for ligand immobilization of copolymers of monomers is unknown

Method used

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  • Substrate for ligand immobilization and method for producing same
  • Substrate for ligand immobilization and method for producing same
  • Substrate for ligand immobilization and method for producing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] (A) method

[0094] (1) Gamma ray irradiation

[0095] A non-woven fabric made of polypropylene (average fiber diameter 3.8 μm, weight per unit area 80 g / m) was used as a carrier 2 )0.108m 2 Seal it together with a deoxidizer in a low-oxygen-permeable bag, and after fully removing oxygen, irradiate with 25kGy of γ-rays at -78°C.

[0096] (2) Grafting reaction

[0097] Dissolve 10.8 g of N-methacryloyloxyethyl-N,N-dimethylammonium-α-N-methylcarboxybetaine and 26.3 mL of glycidyl methacrylate (GMA) in 500 mL of methanol at 40 °C for 60 minutes with nitrogen.

[0098] The above-mentioned non-woven fabric was quickly put into a pressure-resistant glass container, and after decompression, the above-mentioned solution was introduced and allowed to react at 40° C. for 1 hour. After the reaction, the taken out nonwoven fabric was washed with dimethylformamide and methanol, and vacuum-dried at 40° C. to obtain a nonwoven fabric for ligand immobilization (substrate for ligan...

Embodiment 2

[0126] Dissolve 21.5g of N-methacryloyloxyethyl-N,N-dimethylammonium-α-N-methylcarboxybetaine and 26.3mL of glycidyl methacrylate in 500mL in the same manner as in Example 1 The reaction solution was prepared in methanol, and the grafting reaction was carried out to obtain a non-woven fabric for ligand immobilization. As a result of surface analysis by XPS, the molar composition ratio R of CMB was 0.26, and the coverage S was 54%. In addition, the measured specific surface area was 0.50 m 2 / g. A cell adsorption filter was fabricated from this nonwoven fabric, and the amount of antibody immobilized was measured. As a result, 11.6 mg of antibody was immobilized on the cell adsorption filter before washing, and 8.4 mg of antibody was immobilized on the cell adsorption filter after SDS washing. As a result of producing a cell adsorber from this cell adsorption filter and measuring the adsorption rate of cells, the adsorption rate of CD4-positive cells was 99.7% (F1) and 89.0% (...

Embodiment 3

[0128] Dissolve 43.1g of N-methacryloyloxyethyl-N,N-dimethylammonium-α-N-methylcarboxybetaine and 26.3mL of glycidyl methacrylate in 500mL of The reaction solution was prepared in methanol, and the grafting reaction was carried out to obtain a non-woven fabric for ligand immobilization. Based on the results of surface analysis by XP S, the molar composition ratio R of CMB was 0.32, and the coverage S was 54%. In addition, the measured specific surface area was 0.51 m 2 / g. A cell adsorption filter was fabricated from this nonwoven fabric, and the amount of antibody immobilized was measured. As a result, 9.6 mg of antibody was immobilized on the cell adsorption filter before washing, and 8.8 mg of antibody was immobilized on the cell adsorption filter after SDS washing. As a result of producing a cell adsorber from this cell adsorption filter and measuring the adsorption rate of cells, the adsorption rate of CD4-positive cells was 95.1% (F1) and 86.0% (F2). In addition, the ...

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Abstract

The present invention provides a substrate for ligand support in which a copolymer represented by the following formula (1) is bonded to at least a surface of a water-insoluble carrier: [in the formula (1), n and m represent a positive integer, and a value of m / (n + m) is greater than or equal to 0.1 and less than or equal to 0.45, R 1 represents H or CH 3 , R 2 represents an organic group including an electrophilic functional group, and R 3 represents

Description

technical field [0001] The present invention relates to a substrate for immobilizing a ligand for immobilizing an affinity ligand and a method for producing the same. Background technique [0002] In order to remove a specific component from blood, an adsorbent material is widely used clinically, which immobilizes a substance having an affinity for the specific component, that is, a ligand, on a water-insoluble carrier through a covalent bond. These adsorbents are used in such a way that the patient's blood is temporarily taken out of the body, the blood or plasma separated by a plasma separator is passed through the adsorbent and processed, and then the blood is returned to the patient. [0003] Therefore, if the ligand is eluted from the adsorbent, it may directly enter the patient's body and cause various physiological effects. Therefore, an adsorbent having a large elution amount of the ligand cannot be used clinically from the viewpoint of safety. That is, when these ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/24A61K35/14A61M1/36
CPCA61M1/3679C08F255/02B01J20/24A61K35/14C08F8/30A61M1/36B01J20/3276B01J20/3278B01J20/328B01J20/321A61P7/00
Inventor 长泽伸一郎福岛正和
Owner ASAHI KASEI MEDICAL CO LTD
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