Food-poisoning bacteria detection carrier, and method for detecting food-poisoning bacteria

一种食物中毒菌、检测方法的技术，应用在的载体领域，能够解决难以识别菌株、测序无迅速性、鉴定困难等问题
CN102844447AInactive Publication Date: 2012-12-26TOYO SEIKAN KAISHA LTD +1
5 Cites 6 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TOYO SEIKAN KAISHA LTD
Publication Date
2012-12-26
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The disclosed method simultaneously and specifically detects at least two types of food-poisoning bacteria from among Escherichia coli, Listeria, Campylobacter, Vibrio parahaemolyticus, Staphylococcus aureus, Salmonella, and Bacillus cereus. The food-poisoning bacteria detection carrier immobilizes one or at least two probes selected from each of at least two groups from among: an E. coli-detecting first probe group comprising the probes of sequence numbers 1-6 and probes (hereinafter written as "and the like") that are complementary thereto; a Listeria-detecting second probe group comprising the probes of sequence numbers 7-13, and the like; a Campylobacter-detecting third probe group comprising the probes of sequence numbers 14-19, and the like; a Vibrio parahaemolyticus-detecting fourth probe group comprising the probes of sequence numbers 20-24, and the like; a Staphylococcus aureus-detecting fifth probe group comprising the probes of sequence numbers 25-29, and the like; a Salmonella-detecting sixth probe group comprising the probes of sequence numbers 30-40, and the like; and a Bacillus cereus-detecting seventh probe group comprising the probes of sequence numbers 41-49.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The present invention relates to a carrier such as a microarray for detecting food poisoning bacteria, and particularly relates to a carrier for detecting food poisoning bacteria capable of specifically and simultaneously detecting a plurality of food poisoning bacteria, and a method for detecting food poisoning bacteria. Background technique

[0002] Conventionally, the presence or absence of food-poisoning bacteria has been inspected in food inspections, environmental inspections, clinical trials, and animal hygiene. In such inspections, in recent years, DNA contained in inspection samples is amplified by PCR (polymerase chain reaction), and target bacteria are detected from the obtained amplification products.

[0003] At this time, electrophoresis is widely used in the detection of food-poisoning bacteria, thereby determining the presence or absence of target bacteria. In addition, the detected amplification product was purified and sequenced to i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More