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A method for producing lactic acid-producing bacillus subtilis synbiotic feed additive bacterial liquid

A technology of Bacillus subtilis and feed additives, which is applied in the cultivation field of lactic acid-producing Bacillus subtilis, can solve problems such as the fermentation process of Bacillus subtilis bacterial liquid produced by industrialized large-scale fermentation, and achieve high content of probiotics, production The effect of low cost and stable process

Inactive Publication Date: 2014-10-22
CHONGQING ACAD OF ANIMAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Large-scale fermentative production of Bacillus subtilis lactic acid liquid is one of the foundations and keys for the production of new microecological preparations, but there is currently no industrialized large-scale fermentation process for the production of Bacillus subtilis lactic acid bacterial liquid

Method used

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  • A method for producing lactic acid-producing bacillus subtilis synbiotic feed additive bacterial liquid
  • A method for producing lactic acid-producing bacillus subtilis synbiotic feed additive bacterial liquid

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Embodiment 1

[0026] The preparation of embodiment 1 culture medium

[0027] Formula 1: The culture medium formula for lactic acid-producing Bacillus subtilis, which specifically consists of the following components in parts by weight: 1 part of peptone, 1 part of beef extract, 1 part of yeast extract, 15 parts of sodium chloride, and 100 parts of fructooligosaccharide .

[0028] Formula 2: The medium formula of lactic acid-producing Bacillus subtilis, which is composed of the following components in parts by weight: 5 parts of peptone, 3 parts of beef extract, 10 parts of yeast extract, 5 parts of sodium chloride, and 10 parts of fructooligosaccharide .

[0029] Formula 3: The culture medium formula for lactic acid-producing Bacillus subtilis, which consists of the following components in parts by weight: 3 parts of peptone, 2 parts of beef extract, 7 parts of yeast extract, 11 parts of sodium chloride, and 75 parts of fructooligosaccharides .

[0030] The above three formulas are prepa...

Embodiment 2

[0031] The application of embodiment 2 culture medium

[0032] one

[0033] The method for producing lactic acid-producing Bacillus subtilis synbiotics feed additive bacterium liquid by the described culture medium specifically comprises the following steps: producing lactic acid-producing Bacillus subtilis seed liquid (concentration is 3×10 8 cfu / mL) was inoculated into a fermenter equipped with the medium (Formulation 3) for dynamic fermentation at 10 rpm. The fermentation conditions were as follows: after continuous culture at 30°C for 24 hours, then static culture for 96 hours to produce lactic acid Bacillus subtilis synbiotic feed additive bacterial liquid. After counting, the number of bacteria containing lactic acid-producing Bacillus subtilis in the bacterial liquid for synbiotic feed additives of lactic acid-producing Bacillus subtilis is greater than 1.80×10 10 CFU / mL.

[0034] two

[0035] Insert lactic acid-producing Bacillus subtilis strains into the medium (F...

Embodiment 3

[0038] Embodiment 3 produces the method for bacterial liquid of producing lactic acid Bacillus subtilis synbiotics feed additive

[0039] 1. Obtaining method of secondary seed solution

[0040] Specifically include the following steps:

[0041] A) Obtaining the primary seed solution

[0042] Inoculate lactic acid-producing Bacillus subtilis strains into nutrient broth (inoculum size 1ml / 100mL), and culture at 37±1°C for 24 hours; After 1 hour of cultivation, select typical colonies to inoculate several branches of nutrient agar slant, place them in a biochemical incubator at 37°C for 24 hours, and use them as the primary seed solution (concentration: 3×10 8 cfu / mL);

[0043] B) Obtaining of secondary seed liquid

[0044] Take the primary seeds and inoculate them in nutrient broth (inoculum size 2ml / 100mL), place them in a biochemical incubator at 37±1°C for 24 hours, and obtain the secondary seed liquid (concentration: 15×10 8 cfu / mL).

[0045] 2. The method for producin...

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Abstract

The invention belongs to the field of biology, and especially relates to a fermentation method and a fermentation formula of lactic acid-producing bacillus subtilis. The formula comprises the components of, by weight: 1-5 parts of peptone, 1-3 parts of beef extract paste, 1-10 parts of a yeast extract, 5-15 parts of sodium chloride, and 10-100 parts of fructooligosaccharide. The fermentation method comprises the steps that: a seed liquid producing the lactic acid-producing bacillus subtilis is inoculated into fermentation tank loaded with the culture medium. The fermentation condition is that: the materials are continuously cultured under 30-37 DEG C for 24-72h, and is subjected to standing and is cultured for 96-120h, such that the lactic acid-producing bacillus subtilis symbiotic feed additive bacteria solution is obtained. The culturing method of the culture medium can be applied in large-scale productions of the lactic acid-producing bacillus subtilis symbiotic feed additive bacteria solution. The process is stable, probiotic content is high, and production cost is low.

Description

technical field [0001] The invention belongs to the biological field, in particular to a method for cultivating lactic acid-producing Bacillus subtilis. Background technique [0002] With the rapid development of the national economy and the improvement of people's living standards, people have put forward higher and newer requirements for the breeding environment and safe and healthy animal products. In recent years, animal food safety incidents that have been exposed frequently, such as the "lean meat powder incident" and "melamine milk incident", have all dealt a heavy blow to the animal breeding industry and caused a serious crisis of trust. As the world's largest animal breeding country, China's export of animal products plays an important role in the national economy. However, in recent years, some countries such as Europe and Japan have used food safety issues as barriers to refuse the entry of some animal products from my country, which has caused undue damage to our...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/16
Inventor 杨金龙刘作华黄勇付利芝郑华张素辉杨睿沈克飞
Owner CHONGQING ACAD OF ANIMAL SCI