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Formula of nannochloropsis oculata medium and three-stage cultivation method

A medium formula and a technique for Nannochloropsis, which are applied in the field of Nannochloropsis medium formula and tertiary culture, can solve the problems of slow growth, low biomass, unfavorable large-scale cultivation and the like

Inactive Publication Date: 2013-01-02
LINYI UNIVERSITY
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0003] At present, the main problems of large-scale cultivation of Nannochloropsis are slow growth, low biomass, and easy pollution, especially in the rainy season, which is not conducive to large-scale cultivation
The previously used Nannochloropsis culture medium did not add sodium bicarbonate, but my experiments showed that Nannochloropsis can effectively use HCO3- in the culture solution as a carbon source in addition to free CO2 in the air

Method used

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Embodiment Construction

[0015] Culture medium preparation method

[0016] 1 Ferric citrate is difficult to dissolve, you can add a small amount of tap water and warm it to 80-90°C on the stove, and keep stirring until it completely melts;

[0017] 2. Gibberellin is a white crystalline powder, which is difficult to dissolve in water. It can be fully dissolved in alcohol (generally, 50 mL of alcohol or 60-degree white wine is used for more than 1 hour per g), and then diluted with water according to the required concentration.

[0018] 3. During the process of adding nutrient salts, the water needs to be continuously stirred, and various nutrient salts are added in the order provided in the formula to avoid chemical reactions;

[0019] 4 Various nutrient salts can be made into mother liquor with higher concentration first, and then added in sequence;

[0020] 5 Nutrient formula and concentration are the same at each stage of tertiary cultivation.

[0021] A Nannochloropsis culture medium is character...

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Abstract

The invention provides a formula of a nannochloropsis oculata medium and a three-stage cultivation method. The formula is as follows: 7.5*10 <-2>g of NaNO3, 1*10 <-2>g of KH2PO4, 5*10<-1>g of NaHCO3, 2*10 <-4>g of ferric citrate (FeC6H5O7.5H2O), 5*10 <-7>g of VB12, 1*10<-4>g of VB1, 5*10 <-7>g of VH, 5 IU of 920 plant growth hormone (namely gibberellin), 2mL of human urine, and 1000mL of disinfected seawater. The experimental results show that the optimized medium and three-stage cultivation method for culturing nannochloropsis oculata have the advantages of fast growth, high yield, low cost, easy operation and little pollution.

Description

technical field [0001] The invention belongs to the field of marine microalgae cultivation, and in particular relates to a Nannochloropsis culture medium formula and a three-stage cultivation method. Background technique [0002] Nannochloropsis oculata, also known as Nannochloropsis oculata, is a widely distributed seawater algae with a diameter of 2-4 microns, spherical cells, light green, rich in unsaturated fatty acids and other nutrients, and EPA content Accounting for 30% of the total fatty acids. EPA can reduce the content of cholesterol and triglycerides, promote the metabolism of saturated fatty acids in the body, thereby reducing blood viscosity, improving blood circulation, improving tissue oxygen supply and eliminating fatigue, and preventing fat deposition on blood vessel walls, preventing atherosclerosis The formation and development of cerebral thrombosis, cerebral hemorrhage, hypertension and other cardiovascular and cerebrovascular diseases. EPA also plays...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 王培磊
Owner LINYI UNIVERSITY
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