Stenotrophomonas acidaminiphila and application in control of apple tree canker thereof

A technology for apple tree rot disease and oligotrophic monocytogenes, which is applied in the application, microorganism-based method, bacteria and other directions, can solve the problem of not retrieving oligotrophic monocytogenes microacidophilus and the like, and achieves low production cost and prevention and control. Stable effect and simple fermentation process

Active Publication Date: 2013-01-02
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The use of biocontrol bacteria to control plant diseases has been reported at home and abroad. After searching, the applicant has not found any evidence that Ste...

Method used

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  • Stenotrophomonas acidaminiphila and application in control of apple tree canker thereof
  • Stenotrophomonas acidaminiphila and application in control of apple tree canker thereof
  • Stenotrophomonas acidaminiphila and application in control of apple tree canker thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Screening of BJ1 Strain of Biocontrol Bacteria

[0034] 63 strains of bacteria were isolated from the rhizosphere soil of commercial apple orchards in Qixia City, the main apple production area in Shandong Province, and were screened by traditional flat plate confrontation culture method, and then liquid fermentation was carried out on the obtained strains in NB medium , the fermentation filtrate was used to carry out re-screening, and finally the bacterial strain BJ1 with significant antagonistic effect on the apple tree rot pathogen was obtained (results are shown in Table 1).

[0035] The solid plate used is PDA medium, and its components and formula are as follows: Weigh 200g after peeling the potatoes, cut into small pieces and boil in water for 15-20min, add 20g of glucose and 15g of agar powder after filtering through four layers of gauze, and dilute to 1000mL, with natural pH, autoclaved at 121℃ for 20min.

[0036] Identification of BJ1 Strain of Biocontrol Bac...

Embodiment 2

[0044] Inhibitory effect of Stenotrophomonas microacidophilus BJ1 strain on various fruit tree pathogenic fungi

[0045] Biocontrol BJ1 against apple tree rot (V.ceratosperma), peach brown rot (Sclerotinia laxa), cherry dry rot (Phomopsis perniciosa), peach tree rot (V.leucostoma) and apple ring rot (Botryosphaeria berengeriana) ) and other main pathogenic fungi of fruit trees have significant inhibitory effect, showing good broad-spectrum antibacterial properties, the results are shown in Table 1.

[0046] Table 1 Inhibition effect of biocontrol bacteria BJ1 strain on main pathogenic fungi of fruit trees

[0047]

Embodiment 3

[0049] Inhibitory Effect of Stenotrophomonas microacidophilus BJ1 on Apple Tree Rot Pathogen

[0050] (1) Effect of the fermentation filtrate of bacterial strain BJ1 on the germination of conidia of putrefaction fungi: the fermentation filtrate of bacterial strain BJ1 has a significant inhibitory effect on the germination of conidia of putrefaction fungus. Rapid growth (see figure 2 , A); After the BJ1 strain fermentation filtrate was processed, the conidia and germ tubes of the rot pathogen were obviously deformed, showing that the germ tubes of the conidia germinated were very short (see figure 2 , B-C), or the conidia were significantly enlarged, and the germinated germ tube could not continue to elongate (see figure 2 , D), some germinated germ tubes elongate and then expand at the top ( figure 2 , E).

[0051] (2) The effect of strain BJ1 on the mycelium morphology of the rot pathogen: the mycelium of the control rot pathogen is uniform in thickness, with fewer bra...

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Abstract

The invention discloses a stenotrophomonas acidaminiphila and an application in control of apple tree canker thereof, and belongs to the technical field of plant disease biological control. A strain of stenotrophomonas acidaminiphila BJ1 used for apple fungal disease biological control is screened from rhizosphere soil of a commercial apple grove in Xixia, Shandong, is preserved in China center for type culture collection with a preservation number of CCTCC NO: M 2011475. The microbial agent of the invention is prepared by the following steps of: 1) preparation of a medium, 2) activation of the BJ1 strain, 3) liquid culture of the BJ1 strain, 4) preparation of a BJ1 biological control preparation, and 5) dilution. The stenotrophomonas acidaminiphila BJ1 strain of the invention has obvious control effect on apple tree canker, has stable control effect, is environment-friendly, is simple in fermentation process for large-scale production, and low in production cost.

Description

technical field [0001] The invention belongs to the technical field of biological control of plant diseases. Specifically, the invention relates to the classification and identification of bacteria used for the biological control of apple tree rot disease bacteria, the preparation method of fermentation liquid and the prevention and treatment of apple tree rot disease. Background technique [0002] Apple is an important economic crop and export fruit in my country, and its cultivated area and output both rank first in the world. However, diseases seriously restrict the development of my country's apple industry, especially the apple tree rot disease (Valsa ceratosperma), which is widely distributed in my country, has serious damage and is difficult to control. Fruit farmers call it "cancer" of apple trees. The rot pathogen has the characteristics of latent infection. Once the conditions are suitable, the bark of the infected part will quickly rot and necrosis, the tree vigor...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12P1/04C12R1/01
Inventor 王彩霞李保华李桂舫董向丽张清明
Owner QINGDAO AGRI UNIV
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