Kit for detecting H5 avian influenza virus

Abstract

The invention relates to a kit for detecting DNA (deoxyribose nucleic acid) of a product of H5 (H5N1 or H5N2) avian influenza virus through gold nanoparticles after the product is amplified via a loop-mediated isothermal nucleic acid amplification, belonging to the technical field of biological detection. According to the kit, the loop-mediated isothermal nucleic acid amplification technology and a bio nanotechnology are combined, therefore the sensitivity in detection of biomolecule is improved, and a method which can be simply and fast operated along with accuracy without professional instrument and equipment is provided; the kit can be widely applied to detecting high-sensitivity H5 avian influenza virus in the fields of family diagnosis, clinical diagnosis, communicable disease control, environment monitoring, inspection and quarantine and biotechnology and the like. The kit provided by the invention includes two parts which are respectively (1) gold nanoparticles on which molecular probes for distinguishing the special sequence of the H5 avian influenza virus are marked, and (2) the loop-mediated isothermal nucleic acid amplification system for amplifying the H5 avian influenza DNA of a sample to be detected or amplifying the H5 avian influenza RNA in the sample to be detected after carrying out reverse transcription.

Description

technical field [0001] The invention relates to a kit for detecting H5 avian influenza virus DNA or RNA amplified by a loop-mediated isothermal nucleic acid amplification technology by using nano gold particles, and belongs to the technical field of biological detection. Background technique [0002] The high sensitivity, high specificity, simple and rapid detection of genes (DNA) and their transcription products (RNA) is very useful for the early diagnosis and treatment of diseases, as well as in the fields of health and epidemic prevention, environmental monitoring, inspection and quarantine, etc. Significance. In the past, more accurate and sensitive detection methods in this regard include fluorescent labeling, radioactive labeling and other methods. These methods require complex operations and special equipment, and their application range is very narrow. In 1993, Dr. Kary B. Mullis of PE Company invented the polymerase chain reaction (Polymerase Chain Reaction), refer...

AI Technical Summary

Problems solved by technology

Current data show that H5N2 avian influenza, regardless of its high pathogenicity or low pathogenicity, is internationally recognized as a poultry disease and will not infect humans, but it has caused huge losses to the breeding industry

[0008] At present, there is no simple, rapid, easy-to-use and low-cost detection method for H5 (H5N1 or H5N2) avian influenza virus

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