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A chip, preparation method, application and drug screening method

A technology for screening drugs and chips, which can be used in instruments, analytical materials, measuring devices, etc., and can solve problems such as no connecting arms and immobilization of various small molecules

Active Publication Date: 2014-10-29
苏州普芯生命科学技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this specific immobilization strategy is very suitable for immobilizing synthetic compounds, the structures of natural compounds are usually diverse, and it is difficult to immobilize a variety of small molecules on the same chip with a single immobilization method, especially some circular small molecules and without connecting arm for fixing
Therefore, immobilization of natural compounds is a technical bottleneck for small molecule arrays

Method used

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  • A chip, preparation method, application and drug screening method
  • A chip, preparation method, application and drug screening method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Embodiment 1 The preparation of the chip provided by the present invention

[0075] Evaporate or magnetron sputter a 1-2nm chromium film on the surface of a clean glass substrate, and then evaporate or magnetron sputter an about 50nm gold film on the surface of the chromium film.

[0076] Take 30mL of distilled water and heat it to 70-80°C, add 6mL of ammonia water and 6mL of hydrogen peroxide, put it into the gold-plated glass substrate prepared above, treat at 70-80°C for 10 minutes, cool for 10 minutes, add distilled water and ethanol to clean, and blow dry with nitrogen. Prepare a solid support.

[0077] Configure HS-PEG-OCH 3 (molecular weight is 350) and HS-PEG-COOH (molecular weight is 450) ethanol solution of the self-assembled compound, wherein the molar ratio of the two is 10:1, and the total concentration is 1mM. Soak the solid support in the ethanol solution of the above-mentioned self-assembly compound, place it at room temperature for 15 hours, take out ...

Embodiment 2

[0078] Embodiment 2 Preparation of the chip provided by the present invention

[0079] Evaporate or magnetron sputter a 1-2nm chromium film on the surface of a clean glass substrate, and then evaporate or magnetron sputter an about 50nm gold film on the surface of the chromium film.

[0080] Take 30 mL of distilled water and heat it to 70-80 °C, add 6 mL of ammonia water and 6 mL of hydrogen peroxide, put the gold-plated glass substrate prepared above, treat at 70-80 °C for 10 min, cool for 10 min, add distilled water and ethanol to clean, and blow dry with nitrogen. Prepare a solid support.

[0081] Configure HS-PEG-OCH 3 (molecular weight is 350) and HS-PEG-OH (molecular weight is 100) self-assembled compound ethanol solution, wherein the molar ratio of the two is 10:1, and the total concentration is 1mM. Soak the solid support in the ethanol solution of the above self-assembly compound, place it at room temperature for 15 hours, take out the solid support connected with t...

Embodiment 3

[0082] Embodiment 3 Preparation of the chip provided by the present invention

[0083] Evaporate or magnetron sputter a 1-2nm chromium film on the surface of a clean glass substrate, and then evaporate or magnetron sputter an about 50nm gold film on the surface of the chromium film.

[0084] Take 30 mL of distilled water and heat it to 70-80 °C, add 6 mL of ammonia water and 6 mL of hydrogen peroxide, put the gold-plated glass substrate prepared above, treat at 70-80 °C for 10 min, cool for 10 min, add distilled water and ethanol to clean, and blow dry with nitrogen. Prepare a solid support.

[0085] Configure HS-PEG-OCH 3 (molecular weight is 350) and HS-PEG-COOH-NH 2 (molecular weight is 339) ethanol solution of the self-assembly compound, wherein the molar ratio of the two is 10:1, and the total concentration is 1mM. Soak the solid support in the ethanol solution of the above-mentioned self-assembly compound, place it at room temperature for 15 hours, take out the solid ...

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Abstract

The present invention relates to the field of drug screening, and in particular to a chip, preparation method and use thereof and method for screening drug. The chip provided in the present invention has a good capacity for resisting nonspecific protein adsorption, and can immobilize a variety of small molecular substances in parallel, and it is further used for screening drugs having complicated ingredients, and has high sensitivity with only traces of compounds being required for effectively analyzing the bioactivities of small molecular substances and specific disease targets.

Description

technical field [0001] The invention relates to the field of drug screening, in particular to a chip, a preparation method, use and a method for screening drugs. Background technique [0002] Traditional Chinese medicine is the cultural precipitation of our country for thousands of years, and has a long history and cultural origin. However, the development of traditional Chinese medicine still relies on traditional methods for many years and is based on raw materials, lacking systematic modern scientific basic research. The mechanism of action of most traditional Chinese medicines is unclear, the key components of traditional Chinese medicine prescriptions are not clear, and there may be unexpected side effects. These factors have hindered traditional Chinese medicine from entering the mainstream of the international natural medicine market. In order for traditional Chinese medicine to go global and enter the international market, it must realize the modernization of tradi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/55C03C17/28C03C17/38G01N21/552
CPCC03C17/28G01N21/553C03C17/38C03C17/42
Inventor 朱劲松何建安陈新颖彭开美
Owner 苏州普芯生命科学技术有限公司
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