Clothianidin antigen, antibody and application thereof

A clothianidin and antibody technology is applied in the field of immunochemical analysis to achieve the effects of large analysis capacity, high accuracy, and easy popularization and promotion.

Inactive Publication Date: 2013-01-16
NANJING AGRICULTURAL UNIVERSITY
View PDF0 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no immunological res...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Clothianidin antigen, antibody and application thereof
  • Clothianidin antigen, antibody and application thereof
  • Clothianidin antigen, antibody and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] 1 Synthesis of artificial hapten

[0026] Substituting the chlorine atom on the thiazole ring of the clothianidin molecule to synthesize a hapten with a four-atom connecting arm, which not only retains the characteristic group of clothianidin to the greatest extent, but also forms a carboxyl terminal group, which can better follow the protein coupling. The product was purified by mass spectrometry (ESI) and H NMR spectroscopy ( 1 H-NMR) identification. The chemical name of clothianidin artificial hapten is 3-(5-((3-methyl-2-nitroguanidine)-ylmethyl)thiazole-2-mercapto)propionic acid, and its molecular structure is as follows:

[0027]

[0028] 1.1 Synthesis of artificial haptens

[0029] Dissolve 8mmol (0.45g) of KOH in 20mL of ethanol, add 4mmol (0.42g) of β-mercaptopropionic acid and stir, after complete dissolution, add 4mmol (1.02g) clothianidin, stir and reflux at 80°C for 2h. After the reaction, the reaction solution was filtered, and the filtrate was conce...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a clothianidin antigen, an antibody and an application thereof, and belongs to the technical field of immunochemistry analysis. The clothianidin antigen and the antibody of the invention are specially used for clothianidin specific polyclonal antibody preparation, enzyme-linked immunoassay (ELISA), and high-sensitivity and rapid detection of clothianidin residues in environment and agricultural products. A hapten is synthesized by substituting a chlorine atom on a thiazole ring of clothianidin, has a chemical name of 3-(5-((3-methyl-2-nitroguanidine)-yl methyl)thiazole-2-mercapto)propionic acid, and is coupled with bovine serum albumin and ovalbumin to prepare an antigen and an envelope antigen. Newzealand white rabbit is immunized by the immunizing antigen to obtain the specific polyclonal antibody of clothianidin. The established ELISA linear scope is 1.1 microgram/L-2 mg/L, and the detection limit is 1.1 microgram/L. The hapten synthetic technology of the invention is simple and feasible, high in antibody specificity, and suitable for detection and on-site monitoring of mass samples in environment and agricultural products.

Description

1. Technical field [0001] The invention relates to clothianidin antigen, antibody and application thereof, and belongs to the technical field of immunochemical analysis. It is specially used for the preparation of clothianidin polyclonal antibody, and the enzyme-linked immunosorbent assay method (ELISA) for clothianidin residues in the environment and agricultural products. 2. Background technology [0002] Clothianidin, chemical name 1-(2-chloro-1,3-thiazol-5-ylmethyl)-3-methyl-2-nitroguanidine, jointly developed by Sumika Takeda / Bayer, It was registered in Japan in December 2001. The chemical structure is shown in the figure below. Molecular formula: C 6 h 8 CIN 5 o 2 S, relative molecular mass: 249.68, melting point: 176.8°C. Clothianidin is a new type of nicotinic insecticide containing a thiazole ring, which mainly acts on the nicotinic acetylcholine receptors in the post-synaptic membrane of the insect nervous system. Clothianidin is widely used in the prevention...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07K16/06G01N33/53
Inventor 王鸣华李明施海燕
Owner NANJING AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap