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Serratia marcescens and application of serratia marcescens in promoting growth of Chinese sweetgum

A technology of Serratia marcescens and sweetgum, applied in the field of biological fertilizers and microbial fertilizers, can solve the problems of few research reports, and achieve the effect of promoting growth and development and excellent strain resources

Inactive Publication Date: 2013-07-31
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are relatively few research reports on Chinese sweetgum trees

Method used

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  • Serratia marcescens and application of serratia marcescens in promoting growth of Chinese sweetgum
  • Serratia marcescens and application of serratia marcescens in promoting growth of Chinese sweetgum
  • Serratia marcescens and application of serratia marcescens in promoting growth of Chinese sweetgum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Determination of Phosphorus Solubilizing Ability of FXW-NJ2D Plate.

[0022] NBRIP medium: glucose 10g, Ca 2 (PO 4 ) 3 5g, MgCl 2 5g, KCl0.2g, MgSO 4 ·7H 2 O0.25g, (NH 4 ) 2 SO 4 0.1g, distilled water 1000mL, pH 7.0, sterilized, pour plate.

[0023] Spot the FXW-NJ2D strain activated twice on the NBRIP plate with an inoculation loop, and after culturing at 30°C for 5 days, measure the diameter of the phosphorus-dissolving circle and the diameter of the colony, and calculate the ratio of the phosphorus-dissolving circle to the diameter of the colony ( figure 1 ).

[0024] from figure 1 It can be seen from the figure that after the FXW-NJ2D strain was cultured on the NBRIP plate, it could produce obviously opaque phosphate-dissolving circles. After measurement and calculation, the diameter of the phosphorus-dissolving circle is 14.67mm, the diameter of the colony is 4.94mm, and the ratio of the phosphorus-dissolving circle to the colony is as high as...

Embodiment 2

[0025] Example 2: Laboratory phosphorus solubilization test of FXW-NJ2D strain.

[0026] Phosphate Solubilizing Medium A: Glucose 10g, Ca 3 (PO 4 ) 2 5g, MgCl 2 5g, KCl0.2g, MgSO 4 ·7H 2 O0.25g, (NH 4 ) 2 SO 4 0.1g, distilled water 1000mL, pH 7.0.

[0027] Phospholytic medium B: with hydroxyapatite (Ca 10 (PO 4 ) 6 (OH) 2 ) instead of Ca in phosphate-dissolving medium A 3 (PO 4 ) 2 , the other components and contents are the same.

[0028] Phospholytic medium C: ferric phosphate (FePO 4 4H 2 O) instead of Ca in phosphate-dissolving medium A 3 (PO 4 ) 2 , the other components and contents are the same.

[0029] Phospholytic medium D: aluminum phosphate (AlPO 4 ) instead of Ca in phosphate-dissolving medium A 3 (PO 4 ) 2 , the other components and contents are the same.

[0030] Inoculate the activated FXW-NJ2D strain into NA medium (3g of beef extract, 10g of peptone, 5g of sodium chloride, 1000mL of distilled water, pH 7.2~7.4), shake culture at 28°C ...

Embodiment 3

[0032] Embodiment 3: FXW-NJ2D greenhouse pot experiment:

[0033] After activating the strain FXW-NJ2D, use an inoculation loop to pick a small amount of bacteria and inoculate them into a 100mL Erlenmeyer flask containing 50mL of NA medium (3g of beef extract, 10g of peptone, 5g of sodium chloride, 1000mL of distilled water, pH 7.2~7.4), Culture at 29°C with shaking at 200r / min for 48h. Centrifuge the fermentation broth (4°C, 6000r / min) for 5 min, rinse the bacteria with sterile normal saline for 3 times, adjust the bacterial suspension with sterile normal saline (10 8 cfu / mL) to make an inoculum. Inoculate Chinese sweetgum (seedling age 1 year), with the same amount of sterile saline as the control, the inoculation amount is 5mL / plant of seedlings. 10 replicates per treatment were placed in the greenhouse for unified management, the light was 12h / day, and watering was timely.

[0034] The growth of Chinese sweetgum 150 days after inoculation is shown in Table 1. It can be...

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Abstract

The invention discloses a serratia marcescens FXW-NJ2D, which is classified and named as serratia marcescens subsp marcescens. The serratia marcescens is preserved in China Centre for Type Culture Collection with the preservation number of CCTCC M 2012261. The invention further discloses an application of the serratia marcescens for promoting the growth of Chinese sweetgum. Under the condition ofliquid shake-flask culture, the bacterial strain in the invention has a strong dissolving effect on insoluble phosphates such as tricalcium phosphate, iron phosphate, aluminium phosphate and hydroxyapatite; the bacterial stain is prepared into a fungicide, and the fungicide is inoculated in a Chinese sweetgum seedling; the results indicate that the fungicide can obviously promote the growth of Chinese sweetgum; and therefore, an excellent bacterial strain resource is provided for developing a special microbial fertilizer for Chinese sweetgum in the future.

Description

technical field [0001] The invention belongs to the technical field of microbial fertilizers in the field of biological fertilizers, and in particular relates to a high-efficiency phospholytic Serratia marcescens in the rhizosphere of sweetgum sweetgum and an application thereof. Background technique [0002] Chinese sweetgum (Liquidambar formosana) is a large deciduous tree, a light-loving and deep-rooted tree species, distributed in tropical and subtropical regions, and distributed in all provinces south of the Yangtze River. Liquidambar tree has a majestic and beautiful shape, and its leaf color has obvious seasonal changes. It has great ornamental value and is also an excellent tree species for landscaping. In addition, sweetgum is also used in construction, medicine, spices, food raw materials and other industries. With the expansion of the application range of sweetgum, the demand for sweetgum has increased, so it is of great significance to screen efficient phosphoru...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K17/00C12R1/43C09K101/00
Inventor 任嘉红张明艳陈凤毛徐国芳
Owner NANJING FORESTRY UNIV
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