Pseudomonas putida capable of efficiently degrading estrogen and acquisition and application thereof

Abstract

A pseudomonas putida strain capable of efficiently degrading estrogen is named as SJTE-1. Preservation number of the pseudomonas putida strain is CGMCC No. 6585. The pseudomonas putida strain can grow by using natural or synthesizing estrogen as only carbon source, can efficiently degrade estrone (E1), 17-beta estradiol (E2), estriol (E3) and 17-alpha ethinyl estradiol (EE2), and can be used for modifying environments polluted by estrogen. Under the condition that the estrogen is used as the only carbon source, the SJTE-1 can degrade 99% of estrone, 17-beta estradiol, and estriol 1mg / L in initial concentration in 24 hours, can degrade more than 95% of estrone, 17-beta estradiol, and estriol 50mg / L in initial concentration in 168 hours, and degrading rate of 17-alpha ethinyl estradiol 5mg / L in initial concentration is more than 90%. Estrone generated during degrading of 17-beta estradiol is also degraded into estrogen-free active substances.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering and relates to a strain of Pseudomonas putida capable of efficiently decomposing estrogen and its acquisition and application. technical background [0002] As a typical endocrine-disrupting substance in the environment, estrogen will accumulate and enrich in the organism in a direct or indirect manner; after binding to hormone receptors in the body, environmental estrogen will cause nervous system disorders, endocrine disorders, and immune competence in organisms. It has strong carcinogenicity and pathogenicity, directly threatens the survival and reproduction of human society and the biosphere, and has attracted widespread attention from academia and society. Estrone (E1), 17-β-estradiol (E2) and estriol (E3) are natural estrogens secreted by human and animal ovaries, which can be discharged into sewage treatment plants along with animal and human excrement , soil, farmland and othe...

AI Technical Summary

Problems solved by technology

[0004] Although the above-mentioned microorganisms all have the ability to degrade estrogen, there are large differences in their degradation efficiency, degradation cycle, and substrate preference. In practical applications, it is difficult for a single strain to effectively decompose a variety of estrogens.

At the same time, although some microorganisms can effectively decompose estrogen under laboratory simulation conditions, because the concentration of estrogen in the actual environment is only at the ng level, it cannot support its growth at all, thus affecting its degradation efficiency

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