Method for producing hydrogen through intensified anaerobic fermentation

An anaerobic fermentation and fermentation vessel technology, applied in the field of enhanced anaerobic fermentation for hydrogen production, can solve the problems of low hydrogen production rate and low raw material conversion rate, and achieve the effects of simple operation, increased permeability and easy realization.

Inactive Publication Date: 2013-01-16
HENAN UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the low conversion rate of raw materials and low hydrogen production rate, anaerobic fermen...

Method used

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  • Method for producing hydrogen through intensified anaerobic fermentation
  • Method for producing hydrogen through intensified anaerobic fermentation
  • Method for producing hydrogen through intensified anaerobic fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] In a 500mL conical flask as a batch reactor, add 100mL of medium, 0.1mg of resazurin, and then add 5mL of cetyltrimethylammonium bromide solution with a mass concentration of 0.2g / L, mix After uniformity, sterilize at 121°C for 15 minutes, then inoculate 5mL of the fermented liquid from cow dung, and then pass sterile nitrogen into the Erlenmeyer flask and the connected alkaline washing bottle until the color of the fermentation system changes from The pink color becomes colorless (the aeration time is about 10 minutes), and then the sterile nitrogen gas inlet is sealed, and the flask is shaken and cultured under the conditions of 37°C constant temperature and 155r / min. The gas generated by fermentation enters the container with a concentration of 2mol / L sodium hydroxide solution in the alkali washing bottle, and then collect the gas overflowing from the alkali washing bottle, which is the hydrogen produced by fermentation;

[0031] The composition of described culture...

Embodiment 2

[0043] Experimental apparatus used, experimental condition and experimental operation are all identical with embodiment 1, and the concentration of the CTAB that adds is 0.4g / L, and the concentration of sodium hydroxide solution in the alkaline water bottle is 3mol / L.

[0044] Adopt gas chromatography to detect hydrogen content, and compare with the situation that does not add CTAB, the result is as attached Figure 5 shown.

[0045] attached by Figure 5 It is concluded that adding 0.4g / L of CTAB is more than that without adding CTAB, the hydrogen production rate has increased by 15.26%, the maximum hydrogen production rate has increased by 31.10%, and the cumulative hydrogen production has increased by 22.73%; at the same time, although the addition of CTAB makes The stagnation time of hydrogen production is prolonged, but the OD of terminal fermentation 600 and pH had no significant effect, and CTAB had no significant effect on microbial growth and hydrogen production met...

Embodiment 3

[0047] Experimental apparatus used, experimental condition and experimental operation are all identical with embodiment 1, and the concentration of the CTAB that adds is 0.6g / L, and the concentration of sodium hydroxide solution in the alkaline water bottle is 4mol / L.

[0048] Adopt gas chromatography to detect hydrogen content, and compare with the situation that does not add CTAB, the result is as attached Figure 6 shown.

[0049] attached by Figure 6 It is concluded that adding 0.6g / L of CTAB is more than that without adding CTAB, the hydrogen production rate has increased by 18.70%, the maximum hydrogen production rate has increased by 43.91%, and the cumulative hydrogen production has increased by 38.18%; at the same time, although the addition of CTAB makes The stagnation time of hydrogen production is prolonged, but the OD of terminal fermentation 600 and pH had no significant effect, and CTAB had no significant effect on microbial growth and hydrogen production met...

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Abstract

The invention discloses a method for producing hydrogen through intensified anaerobic fermentation. The method comprises the following steps of: adding a culture medium, resazurin and a cetyl trimethyl ammonium bromide solution into a fermentation container, mixing uniformly, sterilizing, inoculating liquid obtained after cow dung is fermented, introducing sterile nitrogen into the fermentation container and an alkaline washing bottle which is connected with the fermentation container so as to displace the air, sealing a sterile nitrogen introduction port, performing shake culture at constant temperature, performing alkaline washing on gas produced by fermentation, and collecting to obtain the hydrogen. By adding cetyl trimethyl ammonium bromide into a fermentation substrate, the problems that the cost of the current hydrogen production by biological anaerobic fermentation is still high and the hydrogen production efficiency is low are solved, and the effects that the accumulated hydrogen yield is improved by 38 percent and the maximum hydrogen production rate is improved by 44 percent are successfully achieved. The method can be simply operated, and is low in cost and easy to implement.

Description

technical field [0001] The invention relates to the field of microbial fermentation hydrogen production, in particular to a method for strengthening anaerobic fermentation hydrogen production. Background technique [0002] The contradiction between the ever-increasing energy demand and the depletion of fossil fuel reserves is intensifying, and hydrogen, as a clean, efficient, and renewable energy carrier, is a very promising alternative energy source. Among many hydrogen production methods, anaerobic fermentation hydrogen production is considered as a hydrogen production method with broad prospects because of its mild conditions, simple equipment and wide range of available raw material sources. However, due to the low conversion rate of raw materials and the low hydrogen production rate, anaerobic fermentation hydrogen production cannot compete with chemical hydrogen production in terms of hydrogen production cost. Improving the conversion rate of raw materials and hydroge...

Claims

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Application Information

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IPC IPC(8): C12P3/00
Inventor 任云利赵爽刘振王键吉尹卫平
Owner HENAN UNIV OF SCI & TECH
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