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Rapid propagation technique for herba dianthi seedling

A technology for Qumai and seedlings, which is applied to the rapid propagation technology of Qumai seedlings and the field of wild Chinese medicinal material reproduction, can solve problems such as affecting the yield and quality of Qumai, no relevant reports on Qumai, and easy occurrence of diseases and insect pests, etc. Climate limitation, effect of increasing reproduction

Inactive Publication Date: 2013-01-30
路帅坤
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  • Summary
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AI Technical Summary

Problems solved by technology

Nowadays, the main cultivation methods of Qumai in China are seed propagation and branch propagation. However, the survival rate of ordinary propagation methods is not high, and it is prone to diseases and insect pests, which affects the yield and quality of Qumai. The growth time is long and the economic benefits are poor, which is far from meeting the production needs.
[0003] Tissue culture is an important way of rapid propagation of fine varieties. There have been many reports on tissue culture of Dianthus species, but there is no relevant report on the rapid propagation of Qumai. Therefore, it is necessary to study the rapid propagation of Qumai and form a set of Specialized rapid propagation technology system

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0017] A. Seed selection and pretreatment: collect well-ripe quince seeds, wash them with water for 15 minutes, blot them dry with filter paper, put them in about 1.0% sodium hypochlorite solution for sterilization for about 3 minutes, and rinse them with sterile water for 5 times;

[0018] B. Break dormancy: refrigerate the sterilized Qumai seeds at a low temperature of 3°C for 10 days, take them out and soak them in 50ppm GA3 for 30 minutes to relieve the dormancy of the seeds and facilitate germination;

[0019] C. Seed germination: Put the dormant seeds into about 70% ethanol solution to sterilize for about 30 seconds, rinse with sterile water 4 times; then inoculate the seeds on the ultra-clean workbench and cultivate them on the following medium: / 2MS+0.1mg / L 6-BA+0.3mg / L Lingfasu+20mg / L inositol+800mg / L agar+500mg / L sucrose+400mg / L banana juice, pH 6.8-7.2; dark culture After 2 days, 14 hours of light per day, 10 hours of darkness, under a cold light lamp with a light i...

example 2

[0022] A. Seed selection and pretreatment: collect well-matured Qumai seeds, wash them with water for 20 minutes, blot them dry with filter paper, put them in about 1.5% sodium hypochlorite solution for sterilization for about 5 minutes, and rinse them with sterile water for 8 times;

[0023] B. Break dormancy: refrigerate the sterilized quince seeds at a low temperature of 6°C for 15 days, take them out and soak them in 50ppm GA3 for 40 minutes to relieve the dormancy of the seeds and facilitate germination;

[0024] C. Seed germination: put the seeds released from dormancy into about 70% ethanol solution to sterilize for about 40 seconds, rinse with sterile water for 5 times; then inoculate the seeds on the ultra-clean workbench and cultivate them on the following medium: / 2MS+0.15mg / L 6-BA+0.5mg lingfasu+50mg / L inositol+1000mg / L agar+600mg / L sucrose+1000mg / L banana juice, pH value 7.3-7.5; dark culture after 2 days , 16 hours of light per day, 8 hours of darkness, under a c...

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PUM

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Abstract

The invention belongs to the technical field of plant tissue culture, and specifically relates to a rapid propagation technique for herba dianthi seedlings. The rapid propagation technique is characterized in that: firstly the plant tissue culture technique is used to cultivate herba dianthi seedlings, and the soilless cultivation technique is used to promote rapid growth of the seedlings. The beneficial effects are that: the plant tissue culture and the soilless cultivation technique are used in combination, the effect is obvious, growth speed is fast, the seedlings can make anti-season growth and be put on the market early, pest probability is relatively small in the growth process, and the quality of herba dianthi is improved.

Description

technical field [0001] The invention relates to the propagation technology of wild Chinese medicinal materials, belongs to the technical field of plant tissue culture, and in particular relates to a rapid propagation technology of Qumai seedlings. technical background [0002] Dianthus superbus (Dianthus superbus), also known as wild wheat, stone column flower, and ten-view flower, is a perennial herb of the family Caryophyllaceae. 30-60cm high, clustered stems, upright, 2 branched branches at the top, enlarged nodes; flowering period from May to June, fruit period from July to October, harvested in summer and autumn, remove impurities, dry in the sun, and cut into sections raw use. The whole herb is used as medicine, it can clear away heart-fire and small intestine fire, conduct heat downward, diuretic and relieve stranguria. itching. Qumai is also a good material for arranging flower beds and flower borders, and can also be potted or cut flowers. Nowadays, the main cul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00
CPCY02P60/21
Inventor 路帅坤
Owner 路帅坤
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