Breeding method for drought-resistant and high-yield wheat

A high-yielding technology for wheat, applied in botany equipment and methods, applications, plant gene improvement, etc., can solve the problems of difficult molecular marker-assisted breeding and the inability to determine favorable alleles of molecular breeding, so as to accelerate the breeding process and improve The effect of choosing efficiency

Inactive Publication Date: 2013-02-06
QINGDAO AGRI UNIV
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Problems solved by technology

At present, there are some urgent problems to be solved in molecular marker-assisted breeding: first, most of the QTL mapping is based on the segregation populations from two parental Therefore, it is impossible to determine the most favorable allele for

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  • Breeding method for drought-resistant and high-yield wheat
  • Breeding method for drought-resistant and high-yield wheat

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[0010] A breeding method for drought-resistant and high-yield wheat includes the following steps:

[0011] 1. Screening of parents for drought resistance traits and yield traits: In this example, the name of the wheat was designated Qingmai No. 6. The selection process of Qingmai 6 mainly aimed at the drought-tolerant-related genes / QTLs and their closely linked molecular markers that have been preliminarily identified in wheat. The reliability of these molecular markers was analyzed using bioinformatics methods and selected QTLs with high contribution rate of phenotypic traits and high genetic effects and their closely linked molecular markers are used as candidate markers. Xgwm319, Xbarc18, Xbarc183, Xbarc200, Xwmc025 and other auxiliary selection markers for wheat root traits, Xrwgs27, Xrwgs28, Xrwgs29, Xrwgs10, Xrwgs11, Xrwgs12 and other auxiliary selection markers for yield-related traits were screened. These markers were used for molecular marker identification of 150 wheat...

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Abstract

The invention discloses a breeding method for drought-resistant and high-yield wheat. That QTL (Quantitative Trait Loci) which are partially used for drought resistance-related traits such as wheat root traits and physiological traits and closely linked molecular markers thereof are used as marker selection indexes difficultly directly selecting traits is determined by a bioinformatics method, and a selection strategy for breeding the drought-resistant wheat is performed by combining molecular marker selection with yield related trait selection. The method comprises the following steps of mainly performing molecular marker selection in parent selection and hybrid early generations (generations F2, F3); performing yield and plant type selection at high generations; determining indexes such as coleoptile length, relative germination rate and nitrate reductase activity, the content of soluble sugar and the content of malondialdehyde and root activity in a seedling period; and mainly determining indexes such as relative water content of flag leaves, the content of soluble protein, protective enzyme systems and yield in later period. Drought-resistant and high-yield No.6 green barley served as a wheat variety is selected by combining drought-resistant trait marker-assisted selection with trait identification.

Description

technical field [0001] The invention relates to a drought-resistant and high-yielding wheat breeding method. Background technique [0002] Wheat is a major food crop widely planted in the world, but about 70% of wheat is distributed in arid and semi-arid areas. Drought has a wide and serious impact on wheat, which can simultaneously affect the photosynthesis, respiratory metabolism, moisture content of wheat Conditions and changes in various enzymes and other physiological metabolic processes. Therefore, research on wheat drought resistance and drought-resistant wheat breeding have become one of the important topics in the world today. Facing the increasing population pressure and the increasing scarcity of water resources, it is an effective way to ensure national food security and promote the sustainable and stable development of wheat production through dryland wheat breeding and cultivating drought-resistant, water-saving, high-yield and high-quality wheat varieties. R...

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
Inventor 穆平林琪王春华张洪生
Owner QINGDAO AGRI UNIV
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