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Method for simultaneously detecting contents of four active ingredients in herba erigerontis preparation

A technology of Dengzhanxixin and active ingredients, which is applied in the field of simultaneous detection of four active ingredients in Dengzhanxixin preparations, can solve the problems of inability to clearly understand the quality of phenolic acid components, inability to quantitatively detect, poor controllability, etc. Ensure the effect of process stability, high accuracy and high precision

Inactive Publication Date: 2013-02-06
昆明振华制药厂有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This standard focuses on controlling the quality of flavonoids. Since the thin-layer chromatography method itself can only detect qualitatively, not quantitatively, it is impossible to clearly understand the quality of phenolic acids. The method is weak and the controllability is poor.
In addition, the important active ingredients in the preparation, such as chlorogenic acid, dicaffeoylquinic acid, etc., have no method for quality control. Therefore, it is necessary to further improve the existing quality standard control methods

Method used

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  • Method for simultaneously detecting contents of four active ingredients in herba erigerontis preparation
  • Method for simultaneously detecting contents of four active ingredients in herba erigerontis preparation
  • Method for simultaneously detecting contents of four active ingredients in herba erigerontis preparation

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Experimental program
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Effect test

Embodiment 1

[0032] 1) Preparation of reference substance solution: take chlorogenic acid, caffeic acid, 1,3-O-dicaffeoylquinic acid, and scutellarin reference substance and dissolve them in methanol to prepare 20 μg / ml of chlorogenic acid per ml, Reference substance solution containing caffeic acid 30 μg / ml, 1,3-O-dicaffeoylquinic acid 12 μg / ml, and scutellarin 100 μg / ml, set aside;

[0033] 2) Preparation of the test solution: Take 1 mL of Erigeron breviscapus mixture (sugar-free type) in a 10 mL measuring bottle, add 4 ml of purified water, dilute to the mark with methanol, shake well, filter, and take the filtrate as the test product solution;

[0034] 3) Determination of the content of the four active ingredients: Precisely draw 10 μL each of the reference substance solution and the test solution, inject it into the liquid chromatograph, measure, record the chromatogram, and calculate by the peak area according to the external standard method; among them:

[0035] The liquid chromato...

Embodiment 2

[0044] 1) Preparation of reference substance solution: take chlorogenic acid, caffeic acid, 1,3-O-dicaffeoylquinic acid, and scutellarin reference substance and dissolve them in methanol to prepare 20 μg / ml of chlorogenic acid per ml, Reference substance solution containing caffeic acid 18 μg / ml, 1,3-O-dicaffeoylquinic acid 10 μg / ml, and scutellarin 100 μg / ml, set aside;

[0045] 2) Preparation of the test solution: Take 1 mL of Erigeron breviscapus mixture (with sugar) in a 10 mL measuring bottle, add 4 ml of purified water, dilute to the mark with methanol, shake well, filter, and take the filtrate as the test product solution;

[0046] 3) Determination of the content of the four active ingredients: Precisely draw 10 μL each of the reference substance solution and the test solution, inject it into the liquid chromatograph, measure, record the chromatogram, and calculate by the peak area according to the external standard method; among them:

[0047] Liquid chromatography us...

Embodiment 3

[0056] 1) Preparation of reference substance solution: take chlorogenic acid, caffeic acid, 1,3-O-dicaffeoylquinic acid, and scutellarin reference substance and dissolve them in methanol to prepare 12 μg / ml of chlorogenic acid per ml, Reference substance solution containing caffeic acid 15 μg / ml, 1,3-O-dicaffeoylquinic acid 6.0 μg / ml, and scutellarin 50 μg / ml, set aside;

[0057] 2) Preparation of the test solution: Take 1 mL of breviscapine oral liquid (sugar-free type) in a 10mL measuring bottle, add 4ml of purified water, dilute to the mark with methanol, shake well, filter, and take the filtrate as the test product solution;

[0058] 3) Determination of the content of the four active ingredients: Precisely draw 10 μL each of the reference substance solution and the test solution, inject it into the liquid chromatograph, measure, record the chromatogram, and calculate by the peak area according to the external standard method; among them:

[0059] The liquid chromatograph ...

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Abstract

The invention relates to a method for simultaneously detecting contents of four active ingredients in a herba erigerontis preparation. Content determination of the four active ingredients is performed after preparation of reference substance solution and test product solution. The method comprises injecting the four active ingredients in a liquid chromatograph, determining, recording a chromatogram, and obtaining the contents of the four active ingredients by calculation according to peak areas in an external standard method. Octadecyl silane bonded silica gel serves as a filling agent of the liquid chromatograph, one or more of acetonitrile or methanol can serve as a mobile phase A, one or more of 0.05%-1.0% of formic acid or acetic acid or phosphoric acid can serve as a mobile phase B, and elution is performed according to the following gradients: time of 0-10min and A at a proportion of 7%-9%; time of 10-24min and A at a proportion of 8%-12%; time of 24-37min and A at a proportion of 9%-16%; time of 37-57min and A at a proportion of 10%-24%; time of 57-60min and A at a proportion of 24%-7%. The method is strong in specificity, high in precision, good in repeatability, high in accuracy and good in durability.

Description

technical field [0001] The invention relates to a method for simultaneously detecting the contents of four active ingredients, chlorogenic acid, caffeic acid, 1,3-O-dicaffeoylquinic acid and scutellarin, in scutellarin preparations. Background technique [0002] Dengzhan Asarum was first recorded in "Southern Yunnan Materia Medica", commonly known as Dengzhanhua, because the flower resembles Dengzhan and the root resembles Asarum, so it is named. Also known as Erigeron breviscapus, Erigeron breviscapus (Erigeron breviscapus), which is also known as Erigeron breviscapus (Yunnan), it is mainly produced in Yunnan, Sichuan, Guizhou, Guangxi, Hunan, Tibet and other provinces and regions. The nature and flavor of Erigeron breviscapus are "sweet, warm, slightly bitter, sweet and pungent". It has the effects of dispelling cold and relieving the exterior, expelling wind and dampness, promoting blood circulation and removing blood stasis, dredging meridian and activating collaterals,...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 梅艳冯春李俊苏莎朱光花
Owner 昆明振华制药厂有限公司
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