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Application of composition containing ginsenoside-Rb3and ginsenoside-Rb2 to treatment of heart and cerebral vascular diseases

A kind of technology of ginsenosides and total ginsenosides, applied in the field of traditional Chinese medicine

Inactive Publication Date: 2013-02-13
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Ginsenosides Rb3 and Rb2 are isomers, the same sapogenins have 4 sugars, 3 of which are six-carbon sugars, all of which are glucose, and each has a five-carbon sugar. The five-carbon sugar of ginsenoside Rb3 is xylose, and ginsenoside Rb2 is a pyran-type arabinose, with slight structural differences and similar physical and chemical properties. Although it is possible to separate the two from the analysis of pharmaceutical experimental facts, it is difficult and increases the cost of the product. Limit and affect the clinical application of this class of drugs

Method used

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  • Application of composition containing ginsenoside-Rb3and ginsenoside-Rb2 to treatment of heart and cerebral vascular diseases
  • Application of composition containing ginsenoside-Rb3and ginsenoside-Rb2 to treatment of heart and cerebral vascular diseases
  • Application of composition containing ginsenoside-Rb3and ginsenoside-Rb2 to treatment of heart and cerebral vascular diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take 20g of total saponins of American ginseng stems and leaves and dissolve in 200mL95% ethanol, then add 200mL0.03% NaOH ethanol solution, stir constantly, produce precipitation, filter, dissolve the residue in water, then add 200mL0.03% NaOH ethanol solution, stir constantly, produce Precipitate, filter, adjust the pH value of the residue to neutral with hydrochloric acid, pass through the resin to desalinate, and evaporate to dryness to obtain 8 g of saponins of the American ginseng stem and leaf diol group.

[0024] Take 8 g of the above-mentioned product, use the first separation method, apply reversed-phase silica gel column chromatography, elute with methanol-water (8:1~9:1), receive the same fraction, recover the solvent, and obtain ginsenoside-Rb3 and Rb2 composition 4.1 g, spectrum see figure 1 , wherein, ginsenoside Rb 3 About 85%, ginsenoside Rb 2 About 15%.

Embodiment 2

[0026] Take 20g of total saponins of American ginseng stems and leaves and dissolve in 200mL95% ethanol, then add 200mL0.03% NaOH ethanol solution, stir constantly, produce precipitation, filter, dissolve the residue in water, then add 200mL0.03% NaOH ethanol solution, stir constantly, produce Precipitate, filter, adjust the pH value of the residue to neutral with hydrochloric acid, pass through the resin to desalinate, and evaporate to dryness to obtain 7.6 g of American ginseng stem and leaf diol group saponins.

[0027] Take 7.6g of American ginseng stem and leaf diol group saponins obtained above, use the second separation method, apply silica gel column chromatography, and elute with chloroform-methanol-ethyl acetate-water (15:22:40:10, upper layer) , received the same fraction, recovered the solvent, and evaporated to dryness to obtain 3.6 g of the composition of the present invention, wherein, ginsenoside Rb 3 About 80%, ginsenoside Rb 2 About 20%.

Embodiment 3

[0028] Example 3 Take 20g of total saponins of American ginseng stems and leaves and dissolve in 250mL95% ethanol, then add 250mL0.03% NaOH ethanol solution, stir constantly, produce precipitation, filter, dissolve the residue in water, then add 200mL0.03% NaOH ethanol solution, stir constantly, produce Precipitate, filter, adjust the pH value of the residue to neutral with hydrochloric acid, pass through the resin to desalinate, and evaporate to dryness to obtain 7.9 g of American ginseng stem and leaf diol group saponins.

[0029] Take 7.9 g of the American ginseng stem and leaf diol group saponins obtained above, use the second separation method, apply silica gel column chromatography, and elute with chloroform-methanol-ethyl acetate-water (15:22:40:10, upper layer) , received the same fraction, recovered the solvent, and evaporated to dryness to obtain 3.9 g of the composition of the present invention, wherein, ginsenoside Rb 3 About 88%, ginsenoside Rb 2 About 12%. ...

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Abstract

The invention provides an application of a composition containing ginsenoside-Rb3 and ginsenoside-Rb2 to the treatment of heart and cerebral vascular diseases, the composition and a preparation method of the composition, wherein the composition contains the ginsenoside-Rb3 as a main component and also contains part of ginsenoside-Rb2 and the like. Experiments on the aspect of pharmacology show that the composition provided by the invention is better than the pure ginsenoside-Rb3 on the aspect of preventing and treating effects for the heart and cerebral vascular diseases; and compared with the pure ginsenoside-Rb3, the composition provided by the invention is simplified in process, reduced in cost, enhanced in activity and not increased in toxicity so as to be suitable for industrial production.

Description

technical field [0001] The invention discloses a ginsenoside-Rb 3 As the main ingredient, it also contains part of ginsenoside Rb 2 etc., and provides a preparation method of the composition; meanwhile, it also discloses the use of the composition in the preparation of medicines for treating or preventing cardiovascular and cerebrovascular diseases, which belongs to the technical field of traditional Chinese medicine. Background technique [0002] The research on ginsenosides began in the early 1960s. So far, Chinese and foreign scholars have successively isolated ginsenoside Ra from the roots, stems, leaves, flowers, fruits, etc. 1 、Ra 2 、Ra 3 , Rb 1 , Rb 2 , Rb 3 , Rc, Rd, Re, Rg 1 , Rg 2 , Rg 3 , Rf , F 1 , F 2 , Rh 1 、Rh 2 More than 50 kinds of tetracyclic triterpenoid ginsenosides. According to its parent structure, it is mainly divided into three categories: Dammarane type saponins, octilolol type (Ocotillol) and side chain-changed-Protopanaxadiol type sa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/704A61P9/00A61P9/10
Inventor 睢大筼陈燕萍于晓风王志才曲绍春马兴元
Owner JILIN UNIV
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