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Bacillus amyloliquefaciens and application thereof in prevention and treatment of walnut anthracnose

A technology of amylolytic spores and walnut anthracnose, applied in the fields of application, pest control, bacteria, etc., to achieve the effect of novel strains, high content of living bacteria, and remarkable effects

Inactive Publication Date: 2013-02-13
宁国市百立德生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Especially the use of Bacillus amyloliquefaciens to control walnut anthracnose has not been reported yet

Method used

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  • Bacillus amyloliquefaciens and application thereof in prevention and treatment of walnut anthracnose
  • Bacillus amyloliquefaciens and application thereof in prevention and treatment of walnut anthracnose
  • Bacillus amyloliquefaciens and application thereof in prevention and treatment of walnut anthracnose

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Isolation and Identification of Bacillus amyloliquefaciens SDF-005 (CGMCC No.6672)

[0023] 1. Separation:

[0024] Bacillus amyloliquefaciens of the present invention adopts the soil dilution separation method to separate and obtain from the walnut forest rhizosphere soil in the southern mountain area of ​​Jinan City, Shandong Province, and the separation method is:

[0025] (1) Soil sample collection: Sampling 200g from the soil surface to 15cm, take several samples at one location, mix them through a coarse sieve, and take 25g for separation;

[0026] (2) Medium plate preparation: Melt NA medium (nutrient agar medium) and PDA (potato dextrose agar medium) medium, pour them into sterilized petri dishes respectively, and cool to make NA and PDA plates;

[0027] (3) Weigh 1g of soil sample into a sterilized test tube, add 100mL of sterile water, shake, and make 10 -2 Soil suspension, let it stand for 5 minutes, and dilute to 10 by 10 times -7 ;

[0028] (4...

Embodiment 2

[0044] Example 2 Verification of indoor effect of Bacillus amyloliquefaciens SDF-005 on walnut anthracnose

[0045] 1. Experimental method

[0046] Using the plate confrontation culture method, the walnut anthracnose bacteria cake (9 mm) to be tested was moved to the center of the plate, and the SDF-005 strain was inoculated at 2 cm around it, and cultured in a biochemical incubator at 27 ° C. Each treatment was repeated 3 times. When the control hyphae covered the plate, the diameter of the colony and the diameter of the inhibition zone were measured by the cross method, and the average value and relative inhibition rate were calculated.

[0047] 2. Results

[0048] It can be seen from Table 2 that SDF-005 has a strong inhibitory effect on the mycelial growth of walnut anthracnose, the diameter of the inhibition zone is 16.17mm, and the mycelial growth inhibition rate is 82.04%.

[0049] Table 2. Determination of indoor antibacterial activity of SDF-005 against walnut anthr...

Embodiment 3

[0051] The preparation of embodiment 3 bacillus amyloliquefaciens SDF-005 wettable powder

[0052] (1) Transfer the preserved Bacillus amyloliquefaciens SDF-005 to the slant of the NA test tube, and place it in an incubator at 26°C for 2-3 days.

[0053] (2) Under sterile conditions, dilute the cultured Bacillus amyloliquefaciens SDF-005 with sterile water at a volume ratio of 1:10, absorb 1 mL, put it in NYBD liquid medium, and culture it on a shaking table at 28°C for 1~ 2d obtains the seed solution;

[0054] (3) Inoculate the seed liquid into a large amount of fermentation medium at a ratio of 3.0% (volume ratio), culture temperature 26°C, initial pH 7.5, liquid volume 70%, culture time 36 hours, ventilation 1:0.8. After the fermentation is completed, the number of bacteria in the fermented liquid can reach 8 billion / g.

[0055] (4) Mix the fermentation broth of Bacillus amyloliquefaciens and bentonite evenly at a mass ratio of 1:9, and dry;

[0056] (5) According to the...

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Abstract

The invention discloses a bacillus amyloliquefaciens and application thereof in prevention and treatment of walnut anthracnose. The strain is bacillus amyloliquefaciens strain SDF-005 (preservation number CGMCC (China General Microbiological Culture Collection Center) No.6672). The strain, fermentation liquid prepared from the strain or bacterium agent prepared from the strain can serve as a biopesticide for the prevention and treatment of the walnut anthracnose. As shown in an experiment, the bacillus amyloliquefaciens strain SDF-005 disclosed by the invention has the characteristics of novelty, high efficiency and high living bacteria; wettable powder of the bacillus amyloliquefaciens strain SDF-005 has an obvious effect on the prevention and treatment of the walnut anthracnose with the prevention and treatment effect reaching 78.22%; and walnut can grow well without the production of phytotoxicity.

Description

technical field [0001] The invention belongs to the field of microorganism application. Specifically, the present invention relates to a strain of Bacillus amyloliquefaciens and its application. Background technique [0002] Anthracnose is one of the important diseases that endanger the healthy development of walnut industry. All walnut producing areas in the world have occurred. It mainly harms fruits, leaves, saplings, young shoots and buds. It has a long incubation period, short onset time, and strong explosiveness. Generally, the diseased fruit rate is 20-40%, and the serious ones are as high as 80%, and the fruit drop rate is over 50%. 10-20 days before harvesting, the fruit will turn black and become rotten quickly, seriously affecting the yield and quality of walnuts, and causing serious economic losses. [0003] For the prevention and treatment of this disease, different agents are used abroad according to the planting area and planting purpose. Nurseries and gre...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00A01P21/00C12R1/07
Inventor 牛赡光王清海刘幸红段春华
Owner 宁国市百立德生物科技有限公司
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