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Enzymatic catalysis method for preparing phytosterol-beta-D-glucoside

A technology for phytosterol and glucoside, which is applied in the field of enzymatic catalysis preparation of phytosterol-β-D-glucoside, can solve the problems of complex operation, harsh reaction conditions and the like, achieves high product safety, improved conversion rate and simple process Effect

Inactive Publication Date: 2014-07-09
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technical solution requires three steps to complete, the operation is complex and the reaction conditions are harsh

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Weigh 2.50g of sitosterol and 1.08g of glucose into a 50mL reaction bottle, add 27mL of acetone and 3mL of acetate buffer with pH5.5 as solvent, seal it, place it in a water bath shaker, and pre-mix at a temperature of 50°C for 30min. Add sitosterol and 5% of the total mass of glucose derived from almond free β-glucosidase, control the rotating speed at 150rpm, and react at a temperature of 50°C. Take samples every 12 hours to detect the reaction process, and react for 72 hours; through high-performance liquid chromatography analysis, The content of sitosterol glucoside in the reaction liquid is 46%; the water-soluble sitosterol derivative-sitosterol glucoside with a purity greater than 95% is obtained through column chromatography separation and purification.

Embodiment 2

[0028] Weigh 2.50g of sitosterol and 1.08g of glucose into a 50mL reaction bottle, add 25mL of acetonitrile and 2.5mL of pH5.0 phosphate buffer as a solvent, seal it, place it in a water bath shaker, and pre-mix for 30min at a temperature of 60°C. Add 8% of the total mass of sitosterol and glucose derived from Aspergillus oryzae free β-glucosidase, control the rotating speed at 150rpm, and the reaction temperature at 60°C, take samples every 12 hours to detect the reaction process, and react for 84 hours: analyzed by high performance liquid chromatography , the content of sitosterol glucoside in the reaction solution is 45%; the water-soluble sitosterol derivative-sitosterol glucoside with a purity greater than 95% is obtained through column chromatography separation and purification.

Embodiment 3

[0030] Weigh 1.68g of phytosterols and 1.44g of glucose into a 100mL reaction bottle, add 34mL of 1,4-dioxane and 4mL of citrate buffer at pH 6.0 as a solvent, seal it, place it in a water bath shaker, and set it at a temperature of 55°C Pre-mix for 30 minutes, add 5% of the total mass of phytosterols and glucose derived from Trichoderma free β-glucosidase, control the rotation speed of 180rpm, and the reaction temperature is 55°C. Samples are taken every 12 hours to detect the reaction process. After 72 hours of reaction: According to high-performance liquid chromatography analysis, the content of sitosterol glucoside in the reaction solution is 50%; the water-soluble sitosterol derivative-sitosterol glucoside with a purity greater than 95% is obtained through column chromatography separation and purification.

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Abstract

The invention relates to an enzymatic catalysis method for preparing phytosterol-beta-D-glucoside. A certain amount of phytosterol is reacted with glucose under the action of biocatalysts to generate phytosterol glucoside which is subjected to column chromatography separation and purification to obtain the phytosterol-beta-D-glucoside. A high conversion rate can be obtained by controlling catalysis processes and reaction conditions. The enzymatic catalysis method is efficient in process and simple to operate, improves product safety greatly, overcomes defects of complicated operation and harsh reaction conditions in prior art, and has broad application prospects.

Description

technical field [0001] The invention relates to a preparation method for enzymatically synthesizing water-soluble phytosterol derivatives in an organic phase, in particular to a preparation method for phytosterol-β-D-glucoside. The application and development of this product involves technical fields such as food, medicine, chemical industry and cosmetics. Background technique [0002] Phytosterols, also known as phytosterols, belong to plant steroid compounds, which are important components of plant cells and also a plant active ingredient. Phytosterols are composed of cyclopentane perhydrophenanthrene as the skeleton and belong to 4-methyl-free sterols, mainly found in nuts, seeds, grains, etc. Phytosterols are generally extracted from the deodorized distillate during vegetable oil refining, and large-scale industrial production has been carried out in China at present. Phytosterols mainly include β-sitosterol, stigmasterol, brassicasterol, and campesterol, among which β...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P33/20
Inventor 贾承胜郭素洁任明星张晓鸣冯骉
Owner JIANGNAN UNIV
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