Rapid propagation method of amorphophallus bulbifer through tissue culture

A technology of tissue culture and konjac, which is applied in the field of plant cell engineering, can solve the problems of long bulb cycle, low bulb reproduction coefficient, intolerance to storage and transportation, etc., and achieve strong vitality and stress resistance, and the quality is not easy to degrade , the effect of not being susceptible to disease

Inactive Publication Date: 2013-02-27
KUNMING UNIV
View PDF10 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there are still some shortcomings in the cultivation of golden konjac, such as large amount of seeds, low bulb propagation coefficient, easy propagation of konjac diseases by cutting into pieces, small amount of seeds, and low variation. With the development of konjac industry, it is urgently needed A large number of high-quality konjac seedlings
Although under laboratory conditions, there has been a breeding method for propagating Amorphophallus bulbils (referring to red Amorphophallus species) through tissue culture (Wu Jinping, Tissue culture and rapid propagation of Amorphophallus bul

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A kind of bulbil class konjac tissue culture rapid propagation method, its steps are as follows:

[0020] (1) Cultivation of sterile seedlings: after routine sterilization of young leaves, petioles and clustered buds of Amorphophallus konjac (Golden Amorphophallus species) plants in Ruili City, Yunnan Province, the leaves were cut into 0.5 or 1.0 cm 2 , petioles and clustered buds were cut into 0.8 or 1cm long, inoculated on conventionally sterilized seedling medium, cultured under 2000lux light, 11h / d, culture temperature 28±1℃, to obtain virus-free aseptic seedlings; seedling medium The components and ratio are: MS basic medium + 0.2mg / L NAA + 0.5mg / L 6-BA + activated carbon 0 g / L or 2 g / L or 3.0g / L, sucrose 30g / L, agar 6 g / L L or 7 g / L or 8g / L, adjust the pH to 5.8 or 6.2;

[0021] (2) Embryoid body induction: the detoxified sterile seedlings obtained in step (1), get the leaves and cut them into 0.5 or 1.0cm 2 , the petiole was cut into 0.8 or 1cm, inoculated on t...

Embodiment 2

[0027] Basically with embodiment 1. The difference is: in the cultivation of sterile seedlings, the bulbil konjac is the red konjac variety from Ruili City, Yunnan Province.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to view more

Abstract

The invention relates to a rapid propagation method of amorphophallus bulbifer through tissue culture and belongs to the technical field of plant cell engineering. The method includes steps of vaccine culture, embryoid induction, seedball induction and seedball mature culture. According to the method, induction development and growth ways of explants are changed through the tissue culture technique, the growth and development way is induced to turn from an organ generation way to an embryoid generation way through addition of corresponding growth regulators, and a lot of mature amorphophallus bulbifer seedballs are obtained through in vitro culture directly. The method has the advantages that the sexual propagation coefficient of the amorphophallus bulbifer is high, the propagation cycle is short, the quality is not easy to degrade, the transplanting process of tissue culture seedlings is simple, in vitro tubers are not prone to diseases and easy to germinate, and a practical technique is provided for factorized rapid production of the amorphophallus bulbifer seedballs.

Description

Technical field: [0001] The invention relates to a method for culturing and propagating bulbil konjac tissue, belonging to the technical field of plant cell engineering. Background technique: [0002] Konjac is a perennial herbaceous plant of the genus Amorphophallus of the Araceae family. It is the only plant population found so far that can synthesize a large amount of glucomannan (konjac glucomannan, KGM). It is mainly distributed in tropical and subtropical regions of Asia and Africa. Only a few Asian countries have cultivation, and China and Japan are more common. It is an important characteristic economic crop in Southwest my country. It has been cultivated and eaten for more than 2,000 years. Its main component, glucomannan, is an excellent pure natural dietary fiber with high economic value. It has been used in food, health care Products, medicine, chemical industry, petroleum, textile and other fields have been widely used, which has driven the development of a larg...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00
Inventor 赵建荣余磊
Owner KUNMING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap