Rapid propagation method of amorphophallus bulbifer through tissue culture

Abstract

The invention relates to a rapid propagation method of amorphophallus bulbifer through tissue culture and belongs to the technical field of plant cell engineering. The method includes steps of vaccine culture, embryoid induction, seedball induction and seedball mature culture. According to the method, induction development and growth ways of explants are changed through the tissue culture technique, the growth and development way is induced to turn from an organ generation way to an embryoid generation way through addition of corresponding growth regulators, and a lot of mature amorphophallus bulbifer seedballs are obtained through in vitro culture directly. The method has the advantages that the sexual propagation coefficient of the amorphophallus bulbifer is high, the propagation cycle is short, the quality is not easy to degrade, the transplanting process of tissue culture seedlings is simple, in vitro tubers are not prone to diseases and easy to germinate, and a practical technique is provided for factorized rapid production of the amorphophallus bulbifer seedballs.

Description

Technical field: [0001] The invention relates to a method for culturing and propagating bulbil konjac tissue, belonging to the technical field of plant cell engineering. Background technique: [0002] Konjac is a perennial herbaceous plant of the genus Amorphophallus of the Araceae family. It is the only plant population found so far that can synthesize a large amount of glucomannan (konjac glucomannan, KGM). It is mainly distributed in tropical and subtropical regions of Asia and Africa. Only a few Asian countries have cultivation, and China and Japan are more common. It is an important characteristic economic crop in Southwest my country. It has been cultivated and eaten for more than 2,000 years. Its main component, glucomannan, is an excellent pure natural dietary fiber with high economic value. It has been used in food, health care Products, medicine, chemical industry, petroleum, textile and other fields have been widely used, which has driven the development of a larg...

AI Technical Summary

Problems solved by technology

[0004] However, there are still some shortcomings in the cultivation of golden konjac, such as large amount of seeds, low bulb propagation coefficient, easy propagation of konjac diseases by cutting into pieces, small amount of seeds, and low variation. With the development of konjac industry, it is urgently needed A large number of high-quality konjac seedlings

Although under laboratory conditions, there has been a breeding method for propagating Amorphophallus bulbils (referring to red Amorphophallus species) through tissue culture (Wu Jinping, Tissue culture and rapid propagation of Amorphophallus bulbils, Journal of Plant Physiology 2007, (5) 2:887 -888 and Long Chunlin et al., Method for Breeding Amorphophallus Amorphophallus Seedlings, Patent Publication No. 1596618 ), but there is no related research on the tissue rapid propagation technology of Amorphophallus konjac varieties

Moreover, the above-mentioned method has the disadvantages of rooting, seedling hardening, transplanting and other multi-step procedures for the rapid propagation of Amorphophallus konjac (red Amorphophallus) and the long period of obtaining Amorphophallus bulbils, slow growth, and intolerance to storage and transportation.