Unlock instant, AI-driven research and patent intelligence for your innovation.

A and B-type blood type resistant sizing reagent

A blood type and reagent technology, applied in anti-blood group antigen immunoglobulin, biological testing, anti-animal/human immunoglobulin, etc., can solve the problems of reagent result interference, increased cost, insufficient agglutination titer, etc., to achieve stability Good, low production cost, high specificity effect

Inactive Publication Date: 2014-09-10
WUHAN KANGLAIBO BIOLOGICAL SCI & TECH CO LTD
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

my country is a large country with a population of 1.3 billion, and the demand for blood typing reagents is very large, but the blood typing reagents currently on the market cannot meet the market demand in terms of quality and quantity
[0003] At present, the cell lines used in the production of anti-A and anti-B reagents in the market are not enough for the agglutination titer of the supernatant antibody saline produced by cell culture. In order to meet the national standard, a small amount of ascites is added to the reagent, which will greatly increase the cost. Antibodies can interfere with reagent results

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A and B-type blood type resistant sizing reagent
  • A and B-type blood type resistant sizing reagent
  • A and B-type blood type resistant sizing reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] [Example 1] Preparation of anti-A and anti-B blood group monoclonal antibody

[0012] 1. Immunization methods and procedures:

[0013] Immunogen: Human A blood group red blood cells and human B blood group red blood cells were used respectively.

[0014] Immune animals (provided by Hubei Experimental Animal Center): BALB / C mice, female, 6-8 weeks old.

[0015] Methods: Type A or B red blood cells were washed twice with normal saline and adjusted to a concentration of 100 million / ml; the immunized animals were injected at multiple points, injected once every other week, and spleen cells were collected three days after the fourth injection was strengthened. in cell fusion.

[0016] 2. Monoclonal Antibody Preparation

[0017] 2.1 Cell Fusion

[0018] Myeloma cells for fusion: the mouse myeloma cell line SP2 / 0 (P2-X-Ag8·6·5·3) (purchased from the Virus Collection Center, Wuhan Institute of Virology, Chinese Academy of Sciences), which is Ig non-secreting type, was ...

Embodiment 2

[0030] [Example 2] Industrialized production of anti-A and anti-B blood typing reagent (monoclonal antibody) and its determination

[0031] 1. Preparation of Hybridoma Cell Culture Supernatant

[0032] The anti-A blood type or anti-B blood type hybridoma cells screened in Example 1 were cultured and passaged in colorless RPMI1640 plus 5% calf serum. After the hybridoma cells grew, a small sample of the supernatant was taken to detect that the hemagglutination titer was greater than or After being equal to the national standard, collect the supernatant and add 3 / 10,000 sodium azide.

[0033] 2. Preparation of anti-A and anti-B blood typing reagent

[0034] Combine the supernatants in 1, add methylene blue to the anti-A supernatant, and make the anti-A blood serum stain blue. Add acridine yellow to the anti-B supernatant, the anti-B blood serum stains yellow, and sterilize and filter to obtain the anti-A and anti-B blood typing reagent.

[0035] 3. Specific determination of a...

Embodiment 3

[0054] [Example 3] Anti-A and anti-B blood typing kit

[0055] [Drug Name]

[0056] Common name: Anti-A, Anti-B blood typing reagent (monoclonal antibody)

[0057] English name: Anti-A and Anti-B Blood Grouping Reagents (Monoclonal Antibody)

[0058] Pinyin: KangA, KangB xuexing dingxing shiji(dankelong kangti)

[0059] [use]

[0060] Exclusively for the identification of human ABO blood type.

[0061] [Test Principle]

[0062] This strain is prepared from the culture supernatant of hybridoma cells that produce anti-A or anti-B blood type antibodies. It adopts the principle of agglutination test and is specially used for identifying human ABO blood type.

[0063] [Main components]

[0064] 1. Anti-A blood typing reagent (blue) 10ml 1 bottle

[0065] 2. Anti-B blood typing reagent (yellow) 10ml 1 bottle

[0066] 3. Instruction manual 1 piece

[0067] [sample request]

[0068] This reagent is suitable for whole blood or 10%, 5% red blood cell suspension.

[0069] [exp...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an A and B-type blood type resistant monoclonal antibody and a hybridoma cell for generating the monoclonal antibody, and has collection numbers of CCTCC C201215 and CCTCC C201216; and the hybridoma cell is respectively obtained by screening after a mice is immunized by a human A blood type red blood cell and a human B blood type red blood cell. The invention also further provides a reagent comprising the monoclonal antibody and a relevant kit.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to producing hybridoma cells using hybridoma technology and monoclonal antibodies and blood typing reagents prepared therefrom. Background technique [0002] Since Landsteinor (1900) discovered the ABO blood type, blood group identification reagents mainly use human serum, which has problems such as large inter-individual differences, unstable quality, high cost, and the need to consume a large amount of human blood. Over the years, scholars from various countries have been working hard to find an ideal reagent to replace human serum, and have successively used animal and plant tissue slurries such as fish, snails, and lentils for research, but due to various problems, they have not been popularized and applied. Voak (1980) and Sacks (1981) successively established hybridoma cell lines secreting anti-A and anti-B monoclonal antibodies by lymphocyte hybridization technology, and used the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/80C07K16/34C12N5/20C12R1/91
Inventor 黄韫韬严兵尹娟刘非
Owner WUHAN KANGLAIBO BIOLOGICAL SCI & TECH CO LTD