BC (Bacterial Cellulose)/PVA (Polyvinyl Alcohol) composite material, as well as preparation method and application thereof

A technology of bacterial cellulose and bacterial cellulose film, which is applied in the fields of medical science and prosthesis, can solve the problem of not meeting the practical application in clinical practice, and achieve the effect of reducing the friction coefficient, satisfying the firm bond and improving the mechanical strength.

Active Publication Date: 2013-03-13
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing BC / PVA composite materials can not meet the clinical practical application

Method used

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  • BC (Bacterial Cellulose)/PVA (Polyvinyl Alcohol) composite material, as well as preparation method and application thereof
  • BC (Bacterial Cellulose)/PVA (Polyvinyl Alcohol) composite material, as well as preparation method and application thereof
  • BC (Bacterial Cellulose)/PVA (Polyvinyl Alcohol) composite material, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] A kind of preparation method of BC / PVA composite material, concrete steps are as follows:

[0055] 1 Preparation of medical bacterial cellulose membrane

[0056] Medium composition: yeast powder: 10g, peptone: 6g, MgSO4: 0.2g, CaCl2: 0.1g, glucose: 20g, coconut milk 1000ml, add 10ml of absolute ethanol after sterilization.

[0057] Cultivation method: Inoculate Acetobacter xylinum into the seed culture medium, shake at 180rpm for 18 hours, inoculate the fermentation medium at a ratio of 10%, and culture for 20 days.

[0058] 2 Purification of natural bacterial cellulose products for medical use

[0059] The bacterial cellulose membrane obtained by fermentation is washed with tap water to remove the culture medium, and then soaked in deionized water until the water is colorless and transparent. Then soak 4 times in 3% SDS, replace the SDS solution once every 12 hours; Pure water once.

[0060] Such as figure 1 As shown, the L929 cell line (mouse fibroblast cell line...

Embodiment 2

[0074] A kind of preparation method of BC / PVA / PVP composite material, concrete steps are as follows:

[0075] 1 Preparation of medical bacterial cellulose membrane

[0076] Medium composition: yeast powder: 10g, peptone: 6g, MgSO4: 0.2g, CaCl2: 0.1g, glucose: 20g, coconut milk 1000ml, add 10ml of absolute ethanol after sterilization.

[0077] Cultivation method: Inoculate Acetobacter xylinum into the seed culture medium, shake at 180rpm for 18 hours, inoculate the fermentation medium at a ratio of 10%, and culture for 20 days.

[0078] 2 Purification of natural bacterial cellulose products for medical use

[0079] The bacterial cellulose membrane obtained by fermentation is washed with tap water to remove the culture medium, and then soaked in deionized water until the water is colorless and transparent. Then soak 4 times in 3% SDS, replace the SDS solution once every 12 hours; Pure water once.

[0080] 3 Preparation of bacterial cellulose wet film block

[0081] A low-te...

Embodiment 3

[0089] Preparation of polyvinyl alcohol / bacterial cellulose / hydroxyapatite integrated cartilage repair products

[0090] 1 Preparation of medical bacterial cellulose membrane

[0091] Medium composition: yeast powder: 10g, peptone: 6g, MgSO4: 0.2g, CaCl2: 0.1g, glucose: 20g, coconut milk 1000ml, add 10ml of absolute ethanol after sterilization.

[0092] Cultivation method: inoculate Acetobacter xylinum into the seed culture medium, shake and cultivate at 180rpm for 16 hours, inoculate the fermentation medium at a ratio of 10%, and culture it statically for 10 days.

[0093] 2 Purification of natural bacterial cellulose products for medical use

[0094] The bacterial cellulose membrane obtained by fermentation is washed with tap water to remove the culture medium, and then soaked in deionized water until the water is colorless and transparent. Then soak 4 times in 3% SDS, replace the SDS solution once every 12 hours; Pure water once.

[0095] 3 Preparation of bacterial cell...

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Abstract

The invention discloses a BC (Bacterial Cellulose) / PVA (Polyvinyl Alcohol) composite material, as well as preparation method and application thereof. The preparation method comprises the following steps of: (1) preparing a medical BC wet film block; (2) preparing a polymer solution preparation, namely, under the conditions of high temperature of 95-130 DEG C and high pressure of 0.05-0.15 MPa, preparing a 20-40wt% PVA water solution, namely the polymer solution; (3) soaking the BC wet film block in the polymer solution; and (4) carrying out freeze thawing crosslinking to form the composite material. According to the PVA / BC composite series material prepared by the preparation method, when the PVA concentration reaches 20%, the modulus of compression reaches more than 30 MPa, especially, when the PVA concentration reaches 28%, the modulus of compression reaches more than 50 MPa, the composite material can be used for preparing a replacement for meniscus or cartilage tissue, or preparing replication products of meniscus or cartilage tissue, and the composite material can meet the mechanical strength requirement of bearing tissues, such as the foot cartilage and the meniscus.

Description

technical field [0001] The invention belongs to biomedical materials. A bacterial cellulose / polyvinyl alcohol composite hydrogel material is prepared, which has the characteristics of high water content, high strength, high biocompatibility, low friction coefficient, viscoelasticity, and fixability. , can be used as a replacement for cartilage, meniscus and other load-bearing tissues. Background technique [0002] Bacterial cellulose (BC) is a biological material with a wide range of uses. Its typical production strain is Acetobacter xylinum. The side wall pores of its cells secrete microfibrils with a diameter of 1.78nm, which consist of microfibrils with a diameter of 3-4nm. The microfibril bundles are then formed into fiber filaments with a bandwidth of 30-100nm and a thickness of 3-8nm, and then interweaved into a multi-level fiber network structure. [0003] Bacterial cellulose as a medical material began to attract attention in the 1980s. After years of research by re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/48
Inventor 王迎军任力李立风
Owner SOUTH CHINA UNIV OF TECH
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