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Preparation method of polydopamine modified alginic acid microspheres

A technology for modifying seaweed and polydopamine, which is applied in the preparation of microspheres, microcapsule preparations, etc., to achieve the effects of cheap raw materials, easy availability of raw materials, and mild preparation conditions

Inactive Publication Date: 2013-03-13
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the research on controlling the swelling of alginate gel balls by in-situ cross-linking has not been reported

Method used

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  • Preparation method of polydopamine modified alginic acid microspheres
  • Preparation method of polydopamine modified alginic acid microspheres
  • Preparation method of polydopamine modified alginic acid microspheres

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Dissolve 5.05mmol of alginic acid in 100ml of phosphate buffer solution with pH=5.5, in N 2 Under protection and mechanical stirring, add 5.05mmol of N-hydroxysuccinimide (NHS) and 5.05mmol of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), After 30-60 minutes, 15.15 mmol of dopamine was added, and after 12 hours of reaction, it was analyzed three times with ethanol to remove unreacted dopamine and NHS / EDC to obtain Alg-DA, which was lyophilized for use.

[0021] Dissolve 50mg of Alg-DA in 5ml of pH=7.5 Tris-HCl buffer solution to obtain a solution with a concentration of 10mg / ml, and self-polymerize to obtain Alg-PDA after magnetic stirring at room temperature for 24 hours.

[0022] Use a syringe with an inner diameter of 0.4mm to draw the Alg-PDA solution into 0.2M 25ml CaCl 2 Add dropwise into the solution at a rate of 1 drop / s, filter the microspheres with gauze after aging for 30 minutes, and wash the microspheres with deionized water for 3 tim...

Embodiment 2

[0024] Dissolve 5.05mmol of alginic acid in 100ml of phosphate buffer solution with pH=5.5, in N 2 Under protection and mechanical stirring, add 5.05mmol of N-hydroxysuccinimide (NHS) and 5.05mmol of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), After 30-60 minutes, 10.1 mmol of dopamine was added, and after 12 hours of reaction, it was analyzed with ethanol three times to remove unreacted dopamine and NHS / EDC to obtain Alg-DA, which was lyophilized for use.

[0025] Dissolve 50mg of Alg-DA in 5ml of pH=7.5 Tris-HCl buffer solution to obtain a solution with a concentration of 15mg / ml, and self-polymerize to obtain Alg-PDA after magnetic stirring at room temperature for 24 hours.

[0026] Use a syringe with an inner diameter of 0.4mm to draw the Alg-PDA solution into 0.2M 25ml CaCl 2 Add dropwise into the solution at a rate of 1 drop / s, filter the microspheres with gauze after aging for 30 minutes, and wash the microspheres with deionized water for 3 time...

Embodiment 3

[0028] Dissolve 5.05mmol of alginic acid in 100ml of phosphate buffer solution with pH=5.5, in N 2 Under protection and mechanical stirring, add 5.05mmol of N-hydroxysuccinimide (NHS) and 5.05mmol of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), After 30-60 minutes, 5.05 mmol of dopamine was added, and after 12 hours of reaction, it was analyzed with ethanol three times to remove unreacted dopamine and NHS / EDC to obtain Alg-DA, which was lyophilized for use.

[0029] Dissolve 50mg of Alg-DA in 5ml of pH=7.5 Tris-HCl buffer solution to obtain a solution with a concentration of 20mg / ml, and self-polymerize to obtain Alg-PDA after magnetic stirring at room temperature for 24 hours.

[0030] Use a syringe with an inner diameter of 0.4mm to draw the Alg-PDA solution into 0.2M 25ml CaCl 2 Add dropwise into the solution at a rate of 1 drop / s, filter the microspheres with gauze after aging for 30 minutes, and wash the microspheres with deionized water for 3 time...

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Abstract

The invention discloses a preparation method of polydopamine modified alginic acid microspheres, which comprises the following steps of: under the protection of nitrogen, grafting dopamine to alginic acid by use of N-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride; polymerizing the synthesized dopamine modified alginic acid under the slight alkaline condition to obtain a polydopamine modified alginic acid solution; dropwise adding the polydopamine modified alginic acid solution into a CaCl2 solution by a syringe; and aging to obtain polydopamine modified alginic acid microspheres. The method disclosed by the invention is simple in process, and the prepared modified alginic acid microspheres have higher swelling resistance and good mechanical properties.

Description

technical field [0001] The invention relates to a preparation method of polydopamine alginic acid microspheres, which belongs to the microsphere preparation technology of in-situ covalent crosslinking. Background technique [0002] Alginic acid is a natural polysaccharide composed of two structural units, guluronic acid (G segment) and mannuronic acid (M segment), which can interact with multivalent cations to form a gel. Due to its good biocompatibility and mild sol-gel process, alginic acid is widely used in the fields of pharmacy and biotechnology as a carrier of drugs, living cells and proteins. [0003] Due to severe swelling of alginate gel, large pore size, biomolecules with a molecular weight less than 300kDa are easy to leak from the gel, etc., which limit the application of alginate gel balls. Many methods for optimizing its embedding performance and controlling its swelling have been reported. For example, after blending alginic acid with biomacromolecules such...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/14C08L5/04C08B37/04B01J13/18
Inventor 姜忠义吴洪王晓莉石家福
Owner TIANJIN UNIV
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