Extraction and culture method of human umbilical cord mesenchymal stem cells

A technology of mesenchymal stem cells and culture methods, applied in the direction of animal cells, vertebrate cells, bone/connective tissue cells, etc., can solve the problems of limited quantity and limited application, and achieve the effect of maintaining the ability of proliferation and differentiation
CN102965338AInactive Publication Date: 2013-03-13SOUTHEAST UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SOUTHEAST UNIV
Publication Date
2013-03-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an extraction and culture method of human umbilical cord mesenchymal stem cells. The umbilical cord tissue is cut up and digested by collagenase I and then transferred into a culture bottle containing a culture medium for continuous culture. The culture medium is a low-sugar DMEM (Dulbecco modified eagle medium) and is added with fetal bovine serum, fibroblast growth factor, epithelial cell growth factor, cell transcription factor and cholesterol. The extraction and culture method disclosed by the invention can culture human umbilical cord mesenchymal stem cells for a long time while maintaining the activity of the stem cells. Through the invention, the problems of excessively fast cell aging and differentiation in current culture of human umbilical cord mesenchymal stem cells are solved, and the human umbilical cord mesenchymal stem cells with the characteristics of stem cells can be obtained for a long time.
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Description

technical field

[0001] The invention relates to a method for extracting and culturing human umbilical cord mesenchymal stem cells. Background technique

[0002] Mesenchymal stem cells (MSCs) are pluripotent stem cells derived from mesoderm and ectoderm, and belong to adult stem cells. Mesenchymal stem cells have the ability of self-renewal and multi-directional differentiation potential, and can differentiate into bone, cartilage, fat, nerve, cardiac muscle and other tissue cells under specific induction conditions. Mesenchymal stem cells exist in a variety of tissues, and mesenchymal stem cells have been successfully obtained from bone marrow, fat, muscle and other tissues. Due to the limited number of mesenchymal stem cells derived from adult tissues and the influence of factors such as patient age and disease, their clinical application is limited. Umbilical cord and umbilical cord blood are an ideal source of mesenchymal stem cells because of their non-invasive way of ...

Claims

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