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Biological agent for preventing and curing cotton diseases and method thereof

A biological agent and disease technology, applied in botany equipment and methods, biocides, chemicals for biological control, etc., can solve difficult-to-prevent and difficult-to-treat problems, and achieve low cost, high cost performance, and good control effect of effect

Inactive Publication Date: 2013-03-20
XINJIANG PRODION & CONSTR CORPS NO 6 DIV AGRI SCI INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The reason why soil-borne diseases are becoming more and more serious and difficult to prevent and cure is that the pathogenic bacteria of soil-borne diseases are generally polyparasitic microorganisms, which can infect several or even hundreds of crops and weeds, making traditional crop rotation, The methods of controlling soil-borne diseases such as variety selection and improved cultivation methods have great limitations

Method used

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  • Biological agent for preventing and curing cotton diseases and method thereof

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Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: prepare biological bacterial agent of the present invention

[0021] First, prepare the fermentation broth of strain ACCC10620: liquid fermentation medium formula: beef extract-1%, peptone-1%, NaCl-0.5%, hydroxyene adenine-0.3g, enoyl adenine-0.3g, xanthan gum -3g, agar-5g, adjust the pH value to 7.2, and make up to 1000g with water; configure the amount of the required fermentation medium according to the above ratio, and then insert the strain into the liquid fermentation medium under aseptic conditions, at 30 After shaking and culturing on a shaker at ℃ for 72 hours, until the number of effective viable bacteria reaches 2.0×10 9 A / ml to make first-grade seeds; then put the cultivated first-grade seeds at a ratio of 3% into fermenters equipped with sterilized and cooled fermentors prepared according to the above ratio for fermentation production, and the fermentation temperature At 30°C, the aeration volume starts at 1:1.2 (v.v.m), increases to 1:1.5 (v...

Embodiment 2

[0026] Embodiment 2: laboratory measures the biological activity of biological bacterial agent of the present invention

[0027] Table 1. ACCC10620, ACCC10700, ACCC40051 and biological bacterial agent of the present invention are to blight, verticillium wilt control effect

[0028]

[0029] a-represents the pathogen of cotton wilt: Fusarium oxysporum f.sp.vesinfectum (Atk.) Snyder et Hansen;

[0030] b- represents the main pathogenic bacteria of Verticillium dahliae: Verticillium dahliae Kleb.

[0031] As can be seen from Table 1, ACCC10620, ACCC10700, and ACCC40051 single bacterial agent are obviously not as good as the control effect of the bacterial agent of the present invention to the control effect of cotton blight and Verticillium wilt. Synergistic and complementary effects, the synergistic effect is obvious.

[0032] After diluting the above-mentioned biological agent 1000 times in the field, apply it to the roots of cotton by drip irrigation, and apply it every 2...

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Abstract

The invention relates to a biological agent used for preventing and curing cotton wilt and cotton greensickness and the preparation method of the biological agent used for preventing and curing cotton wilt and cotton greensickness. The biological agent adopts three bacterial strains, ACCC10620: Bacillus subtilis(Ehrenberg)Cohn, ACCC10700;Bacillus subtilis(Ehrenberg)Cohn and ACCC10700;Suction Streptomyces jinggang variants (Streptomyces hygrospinosus var.jinggangensis). The biological agent which is formed by ferment and mixing with other adjuvant materials has the advantage of being capable of efficiently preventing and curing cotton wilt and cotton greensickness.

Description

technical field [0001] The invention relates to a bactericide, in particular to a biological bactericide, in particular to a biological bactericide which can be used for preventing and treating cotton wilt and cotton verticillium wilt and a preparation method thereof. Background technique [0002] Cotton fusarium wilt and cotton verticillium wilt are diseases caused by pathogens in the soil attacking crops from the roots or stems of crops under favorable conditions. The pathogens are mainly transmitted through soil organic fertilizers, irrigation water or natural running water. Severely damaged plots can reduce crop yields by 30%-50%. The leaders and researchers of the Agricultural Science Institute of the Sixth Agricultural Division of Xinjiang have been researching and exploring the use of biotechnology and products to solve this problem for many years. [0003] The reason why soil-borne diseases are becoming more and more serious and difficult to prevent and cure is that...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P3/00
Inventor 李东方
Owner XINJIANG PRODION & CONSTR CORPS NO 6 DIV AGRI SCI INST
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