Related protein HTDF (high tillering and dwarf) for tillering and plant length of rice, and encoding gene and application thereof

A coding and rice technology, applied in the field of rice genetics and breeding, to achieve the effect of improving rice yield

Inactive Publication Date: 2013-03-20
CHINA THREE GORGES UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although some progress has been made in the research on the molecular mechanisms controlling rice tillering and plant height and other important agronomic traits, there are still a large number of genes controlling tillering and plant height to be discovered and studied, and the genetic improvement of rice plant type is urgently needed. genetic resources

Method used

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  • Related protein HTDF (high tillering and dwarf) for tillering and plant length of rice, and encoding gene and application thereof
  • Related protein HTDF (high tillering and dwarf) for tillering and plant length of rice, and encoding gene and application thereof
  • Related protein HTDF (high tillering and dwarf) for tillering and plant length of rice, and encoding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1. Acquisition of Rice Tiller and Dwarf-related Proteins and Their Encoding Genes

[0029] 1. Genetic analysis of rice tiller and dwarf related gene HTDF

[0030] We found a new rice mutant with short stems and multiple tillers in the field. Compared with the wild type Taipei 309 (TP309), this mutant mainly exhibits traits such as more tillers and dwarfing plants, so we named it htdf (high tillering and dwarf)( figure 1 ). At the rice maturity stage, the average number of tillers in the mutant htdf (35.43±5.39) was significantly higher than that of the wild-type variety Taipei 309 (19.3±1.21) ( figure 1 ); the plant height of the mutant was also significantly reduced, about 73.8% of the wild-type variety ( figure 2 ).

[0031] Orthogonal and reciprocal F of mutant htdf to Taipei 3091 The plant height and tillering traits of the generation plants were similar to those of Taipei 309, indicating that F 1 The plant height and tillering traits of the generati...

Embodiment 2

[0045] The expression analysis of embodiment 2, HTDF

[0046] In order to study the expression pattern of the HTDF gene, it was analyzed by quantitative RT-PCR in the roots, seedlings, nodes, internodes, leaves, ears, calli, etc. of the japonica rice variety Taipei 309 (Oryza sativa L. subsp. expression in the organization. The fluorescence signal (expressed in Ct value) of HTDF gene and the fluorescence signal (expressed in Ct value) of Actin1 gene (internal reference gene) are according to the formula (relative expression amount=2 -ΔCt , where ACt=Ct 目的基因 -Ct 内参基因 ) calculated value as the relative expression level of HTDF gene, the results are shown in Figure 6 . The results showed that the HTDF gene was expressed in all the tested tissues, and the expression level in ear and callus was relatively high.

Embodiment 3

[0047] Embodiment 3, cultivating the rice that dwarf stalk and / or tiller number increase

[0048] Using the KOME full-length cDNA clone (No. AK108569) as a template, PCR amplification, enzyme digestion, and connection to the expression vector pTCK303 with Ubiquitin promoter, the interference vector RNAi-HDTF was constructed, and the RNAi-HDTF Transformed into Agrobacterium tumefaciens strain LBA4404 (Cat. No. 18313015, Invitrogen Company), and screened to obtain a recombinant Agrobacterium strain containing the interference vector RNAi-HDTF, using wild-type rice variety TP309 mature embryos to induce callus. Agrobacterium transformation, a total of 8 independent T0 strains were obtained.

[0049] Statistical analysis of the plant type of T1 transgenic plants transgenic for RNAi-HDTF gene and control TP309 plants showed that the number of tillers and plant height of T1 transgenic plants transgenic for RNAi-HDTF gene increased significantly. It shows that using the method of ...

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Abstract

The invention discloses related protein HTDF (high tillering and dwarf) for tillering and plant length of rice, and an encoding gene and application thereof, and belongs to the technical field of genetic breeding of the rice. The related protein HTDF for tillering and plant length of the rice and the encoding gene thereof provided by the invention can cause increase of the tillering quantity of the rice and reduction of the plant length under the condition of losing functions or reducing the expression quantity. Therefore, the plant type of the rice can be effectively adjusted, controlled and improved by the gene through a genetic engineering method; and the related protein HTDF plays an important potential role in improvement of the yield of the rice and cultivation of new variety of the plant.

Description

technical field [0001] The invention belongs to the technical field of rice genetics and breeding, and relates to a rice tiller and plant height-related protein HTDF, its coding gene and its application. Background technique [0002] Rice is one of the most important food crops in the world, and improving rice yield has always been the main purpose of rice breeding. Plant type is an important factor affecting the high yield of rice. The number of tillers and plant height are important components of rice plant type. The strength of rice tillering ability is directly related to the quantity and quality of effective panicle number. Appropriate tillering ability has always been a key factor in rice. A main indicator of ideal plant shape breeding; in the process of rice high-yield breeding and plant shape improvement, tiller number and plant height have always been the focus of attention of breeders. [0003] Through the cloning and functional research of some genes that affect ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/55C12N9/16C12N15/63C12N15/11C12N5/10C12N1/21C12N7/01C12N15/82A01H5/00
Inventor 张德春陈彩艳刘伟朱玉新陈发菊李晓玲
Owner CHINA THREE GORGES UNIV
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