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Crossbreeding method for hollies

A technology of hybrid breeding and holly, which is applied in the direction of botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of long breeding cycle, pollen pollution, heavy workload, etc., to avoid tedious work, shorten the breeding cycle, Workload saving effect

Inactive Publication Date: 2013-03-27
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The traditional method of hybridization of holly plants requires tedious procedures such as bagging and sand storage
Not only the breeding cycle is long, but the workload is also relatively large, and the probability of pollen pollution is relatively high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. On July 1, 2009, select the top shoots of the year's holly holly (female plant) and the whole brim (male plant) as cuttings, and select a suitable cutting pool for cuttings. The size of the cutting pool is about 4m 2 , the cutting density is 5cm×5cm, 400 plants of two varieties are cut respectively, and the treatment method is 1000mg / L IBA soaking the base of the cuttings for 50s. 60 days after cutting, spray low-concentration potassium fertilizer (350mg / LKH 2 PO 4 ), and at the same time make sulfuric acid paper as a panel box to cover the whole cutting pool.

[0024] 2. Harvesting of seeds: In late November 2009, when the red rinds were softened, the fruit began to mature. On November 30, the seeds with red rinds were picked.

[0025] 3. Peel treatment: After drying for a week, remove the peel by rubbing to obtain 1000 clean seeds.

[0026] 4. Radiation treatment: the hybrid seeds obtained in step (3) are subjected to 30Gy dose of cobalt 60 radiation treatment; ...

Embodiment 2

[0030] 1. On July 1, 2010, select the top shoots of Dabieshan holly (female plant, I. dabieshan) and European holly (male plant, I. Nellie) as cuttings, and select a suitable cutting pool for cuttings. Size about 4m 2 , the cutting density is 5cm×5cm, 400 plants of two varieties are cut respectively, and the treatment method is 1000mg / L IBA soaking the base of the cuttings for 50s. 60 days after cutting, spray low-concentration potassium fertilizer (350mg / LKH 2 PO 4 ), and at the same time make sulfuric acid paper as a panel box to cover the whole cutting pool.

[0031] 2. Harvesting of seeds: In late November 2010, when the red peels were softened, the fruit began to mature. On November 30, the seeds with red peels were picked.

[0032] 3. Peel treatment: After drying for a week, remove the peel by rubbing to obtain 1000 clean seeds.

[0033] 4. Radiation treatment: the hybrid seeds obtained in step (3) are subjected to 30Gy dose of cobalt 60 radiation treatment;

[0034...

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PUM

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Abstract

The invention discloses a crossbreeding method for hollies. The method includes pollinating, picking seeds, processing seed coats, subjecting the processed seeds to cobalt irradiation mutagenesis processing, performing tube culture on the irradiated seeds, and performing habituated culture on seeds in tubes. The reproduction method breeding the hollies is simple and easy to implement, and the breeding culture cycle can be shortened to 12 months.

Description

technical field [0001] The invention specifically relates to a method for hybrid breeding of holly (Ilex) plants, and belongs to the technical field of woody plant seedling breeding. Background technique [0002] Ilex belongs to the Ilex family (Aquifoliaceae), about 400 species, distributed in North and South America, tropical and temperate Asia, several species are produced in Europe and Oceania, about 118 species in my country, rich in provinces south of the Yangtze River, some species The wood can be used for carving and furniture, some for garden ornamental use, and some kinds of leaf decoction can be paste for paper pulp. Trees or shrubs; leaves alternate, a few opposite evergreen or deciduous, dentate or spiny serrate; flowers unisexual, sometimes polygamous, in axillary cymes or umbels; calyx lobes, petals and Stamens usually 4; ovary superior, 3 to many locules, each locule with 1-2 pendulous ovules, born on axial placenta; fruit a globose, berrylike drupe. [0003...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H4/00A01C1/00
Inventor 李乃伟陆小清李云龙王传永
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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