Production method of biological fish meal through microbial fermentation

A technology for microbial fermentation and fermentation substrates, applied in animal feed, animal feed, applications, etc., can solve the problems of poor palatability of plant protein sources, unbalanced amino acid composition, and unsatisfactory application effects, so as to improve immunity, The effect of reducing disease and improving digestive utilization

Active Publication Date: 2013-03-27
张有聪
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the application effect of pure plant protein instead of fish meal is not ideal. On the one hand, there are anti-nutritional factors in the plant protein source; Contains unknown growth factors, lower digestibility than fishmeal

Method used

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  • Production method of biological fish meal through microbial fermentation
  • Production method of biological fish meal through microbial fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] see figure 1 , prepare the biological fish meal of the present embodiment according to the following method:

[0059] 1. Weigh 500kg of corn gluten powder, 50kg of rapeseed meal, 50kg of alcohol meal DDGS, and 400kg of vermicelli protein powder.

[0060] 2. Pickling and enzymatic hydrolysis of corn gluten powder, the specific method is as follows:

[0061] A. Enzymolysis: Pour 600kg of corn gluten powder into a 5000L enzymolysis tank, add water and mix materials according to the ratio of material to water 1:5, then adjust the pH to 4.8 with 0.2mol / L hydrochloric acid, and dissolve the substrate liquid Heat to 52°C, add cellulase and acid protease according to the dosage of 30U / g and 5000U / g, and enzymatically hydrolyze at constant temperature for 60min;

[0062] B. Pickling: adjust the pH of the enzymolyzed substrate liquid to 3.8 with 0.2mol / L hydrochloric acid, stir at 80r / min for 60min at 31°C, centrifuge after the protein aggregates and precipitate, and remove the...

Embodiment 2

[0094] see figure 1 , prepare the biological fish meal of the present embodiment according to the following method:

[0095] 1. Weigh 500kg of corn gluten powder, 100kg of rapeseed meal, 100kg of alcohol meal DDGS, and 400kg of vermicelli protein powder.

[0096] 2. Pickling and enzymatic hydrolysis of corn gluten powder, the specific method is as follows:

[0097] A. Enzymolysis: Pour 500kg of corn gluten powder into a 5000L enzymolysis tank, add water and mix materials according to the ratio of material to water 1:5, then adjust the pH to 4.8 with 0.2mol / L hydrochloric acid, and dissolve the substrate liquid Heat to 52°C, add cellulase and acid protease according to the dosage of 30U / g and 5000U / g, and enzymatically hydrolyze at constant temperature for 60min;

[0098] B. Pickling: adjust the pH of the enzymolyzed substrate liquid to 3.8 with 0.2mol / L hydrochloric acid, stir at 80r / min for 60min at 31°C, centrifuge after the protein aggregates and precipitate, and remove t...

Embodiment 3

[0109] see figure 2 , prepare the biological fish meal of the present embodiment according to the following method:

[0110] 1. Weigh 600kg of corn gluten powder, 50kg of rapeseed meal, 50kg of alcohol meal DDGS, 400kg of vermicelli protein powder, and 100kg of fish soluble pulp.

[0111] 2. Pickling and enzymatic hydrolysis of corn gluten powder, the specific method is as follows:

[0112] A. Enzymolysis: Pour 600kg of corn gluten powder into a 5000L enzymolysis tank, add water and mix materials according to the ratio of material to water 1:5, then adjust the pH to 4.8 with 0.2mol / L hydrochloric acid, and dissolve the substrate liquid Heat to 52°C, add cellulase and acid protease according to the dosage of 30U / g and 5000U / g, and enzymatically hydrolyze at constant temperature for 60min;

[0113] B. Pickling: adjust the pH of the enzymolyzed substrate liquid to 3.8 with 0.2mol / L hydrochloric acid, stir at 80r / min for 60min at 31°C, centrifuge after the protein aggregates an...

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Abstract

The invention provides a production technology of biological fish meal by microbial fermentation method. The production method comprises the following steps of performing enzymolysis and scouring on corn gluten meal in a fermentation substrate to extract protein, and mixing the fermentation substrate with other raw materials in a certain proportion; carrying out digestion and sterilization and on the mixed fermentation substrate through a conditioner, adding water for reducing temperature, and adding purified fulvic acid which is 1 to 3 percent by weight of the fermentation substrate, and inoculating a mixed strain fermentation bacterium liquid consisting of 3 bacterium liquids; filling the inoculated fermentation substrate into a mobile fermentation track through a kick-out device, fermenting for several days indoors in the fermenting room; baking the fermented material at a low temperature, and crushing to obtain the biological fish bone finished product. The fermentation substrate consists of the following components in percentage by weight: 40 to 60 parts of corn gluten meal, 5 to 10 parts of rapeseed meal, 5 to 10 parts of alcohol meal, and 30 to 40 parts of vermicelli albumen powder. When the product is prepared to be supplied to a monogastric animal, the fish soluble condensed, which is 10% by weight of the fermentation substrate; and the three bacterium liquids are respectively monilia tropicalis, lactobacillus casei and bacterium liquid of glossy ganoderma.

Description

technical field [0001] The invention relates to a method for producing biological fish meal through microbial fermentation, and belongs to the field of microbial feed additives. Background technique [0002] Fishmeal is the most important protein source in aquafeed, and it is also the substance that accounts for the largest proportion of aquafeed cost. It is known as the "king of protein" because of its high protein content, rich in essential amino acids for animals, good digestibility, and easy digestion and absorption by animals. With the signing of China-Korea and China-Japan fishery agreements, the reduction of world fish catches and the sharp rise in the price of imported fishmeal have brought severe challenges to the feed industry. Seeking cheap and stable protein that can partially or completely replace fishmeal can alleviate the shortage of fishmeal supply on the one hand, and is also an important measure to reduce feed costs on the other hand. Therefore, how to us...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00A23K1/06A23K1/14A23K10/38
CPCY02P60/87
Inventor 张有聪郝永清任国军史彬林
Owner 张有聪
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