Method for improving fermentative hydrogen production activity of anaerobic bacteria
An anaerobic bacteria and active technology, applied in the field of organic wastewater biological treatment, can solve the problems of long stagnation period and low hydrogen production efficiency, and achieve the effects of shortening the stagnation period, increasing the hydrogen production rate, and shortening the fermentation period
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Embodiment 1
[0019] Example 1 Experiment of Silver Nanoparticles Promoting Hydrogen Production by Anaerobic Fermentation
[0020] The anaerobic mixed hydrogen-producing bacteria used in this example are anaerobic activated sludge taken from a sewage treatment plant, baked at 100°C for 2 hours, then boiled for 30 minutes, and then transferred to an anaerobic reactor for 30 days of fermentation , and then the fermentation broth was transferred to a continuous stirred tank reactor for continuous culture, the hydraulic retention time was 24 h, the temperature was controlled at 35 °C, and the pH was not controlled, and the culture was acclimatized for 60 days.
[0021] The composition of the fermentation broth: add 20 mL of mixed hydrogen-producing bacteria liquid to a 120 mL fermenter, 40 mL of medium (1000 mL of distilled water contains the following ingredients: glucose 20 g; beef extract 4 g; peptone 10 g; Na 2 CO 3 4 g; K 2 HPO 4 ·3H 2 O 250 mg; MgCl 2 ·6H 2 O 200 mg; CuSO 4 ·5H ...
Embodiment 2
[0024] Example 2 An experiment of anaerobic bacteria activating alkali shock inactivating the hydrogen production activity of bacteria
[0025] The difference between the anaerobic mixed hydrogen-producing bacteria used in this example and Example 1 is that when using a continuous stirred tank reactor for continuous culture, the hydraulic retention time is 24 h, the temperature is controlled at 35 °C, and the pH value is adjusted to 12. Cultivate and acclimatize for 15 days, so that the hydrogen-producing fermentation of bacteria is inhibited and hydrogen production stops.
[0026] The composition of the fermentation broth: Add 20 mL of the above-mentioned mixed hydrogen-producing bacteria liquid that stopped hydrogen production, 40 mL of the medium (the composition is the same as that in Example 1), NH 4 Cl 20 mL (to make the final concentration of nitrogen element 0.3-1.1 g / L respectively), the final concentration of nano-silver is 20 nmol / L (the volume of nano-silver coll...
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