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Twig cuttage breeding method for ginseng fruit

A technology of young branch cutting and ginseng fruit, applied in the field of young branch cutting propagation of ginseng fruit, can solve the problems of low reproduction coefficient, difficulty in seed reproduction and the like, and achieve the effect of being beneficial to healing

Inactive Publication Date: 2013-04-03
陈志宏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The object of the present invention is to provide a kind of twig cutting propagation method of ginseng fruit, which solves the difficulty of seed propagation when traditional ginseng fruit adopts sexual reproduction; adopts asexual propagation to obtain seedlings, but the disadvantage of low propagation coefficient, the present invention is from ginseng fruit cutting propagation material selection and cutting

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Selection and sterilization of explants: cut terminal buds or stems with axillary buds from potted plants or field plants as explants, and use them as explants for detoxification tissue culture after sterilization;

[0023] 2. Stem tip culture: Under aseptic conditions, the sterilized terminal buds or stem segments with axillary buds are extracted under a 40-fold binocular dissecting microscope, and a 0.2mm-sized shoot tip with 1 leaf primordium is extracted and inoculated in On the induction medium (MS+BA 1mg / L+IAA 0.1mg / L+sucrose 30g / L), after 20 days of culture under the culture condition, the shoots were induced to form from the shoot tip, and after another 20 days of culture, the stem continued to elongate. Seedlings with about 3 nodes and a height of 5 cm;

[0024] 3. Micro-cutting culture: cut the induced seedlings into 3 sections and transfer them to the micro-cutting medium (MS+BA 0.5mg / L+NAA 0.1mg / L+sugar 30g / L), and cultivate them under the culture conditi...

Embodiment 2

[0031] 1. Selection and sterilization of explants: cut terminal buds or stems with axillary buds from potted plants or field plants as explants, and use them as explants for detoxification tissue culture after sterilization;

[0032] 2. Stem tip culture: Under aseptic conditions, the sterilized terminal buds or stem segments with axillary buds are extracted under a 40-fold binocular dissecting microscope to extract 0.3mm-sized shoot tips with 2 leaf primordia, and inoculate them in On the induction medium, after culturing under culture conditions for 30 days, shoots were induced from the shoot tip to form shoots, and after culturing for another 30 days, the stems continued to grow, and were cultivated into seedlings with about 5 nodes and a height of 7 cm;

[0033] 3. Micro-cuttage culture: cut the induced seedlings into 5 sections and transfer them to the micro-cuttage medium, and cultivate them for 45 days under culture conditions, and then cultivate them into 5-section, 7cm-...

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PUM

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Abstract

The invention discloses a twig cuttage breeding method for a ginseng fruit, relates to a fruit pollution-free cultivation method and belongs to the agricultural technical field. The twig cuttage breeding method disclosed by the invention is characterized in that a terminal bud or a stem section with an axillary bud is cut from a potted plant or a big-field plant and is taken as an explant, sterilization is carried out, and the explant used for virus-free tissue culture is used for carrying out steam tip culture, micro cuttage culture, strong seedling culture, micro cuttage culture, rooting culture, transplanting and field planting culture. The twig cuttage breeding method disclosed by the invention has the beneficial effects that micro graft material can easily realize mass propagation in a smaller space and shorter time without being influenced by seasons and external climate; and the adopted culture medium, tissue culture conditions and a micro grafting device are applicable to micro grafting culture of a virus-free seedling of the ginseng fruit, healing of a grafting port is facilitated, and the average grafting success rate is more than 90%.

Description

[0001] technical field [0002] The invention relates to a pollution-free cultivation method of fruit, which belongs to the field of agricultural technology, in particular to a ginseng fruit twig cutting propagation method. Background technique [0003] Ginseng fruit, also known as Xiantao and Yanguo, is a perennial semi-lignified herb of the family Solanaceae, and is a perennial herb that eats flesh. Its nutrients contain protein, low sugar, low fat, but also rich in vitamin C, and a variety of trace elements necessary for the human body, especially the content of selenium and calcium is much higher than that of other fruits and vegetables; the trace elements include selenium , molybdenum, cobalt, iron, zinc, molybdenum contained in molybdenum has anti-cancer and anti-cancer effects, cobalt can prevent coronary heart disease, high blood pressure is also helpful, selenium is a strong oxidant, can maintain the normal physiological functions of the body, activate the human bo...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈志宏
Owner 陈志宏