Semen lepidii anti-tumor effective component extract, as well as preparation method and application thereof

A technology of Fructus Tingli and its effective components, applied in the field of extracts of Fructus Tingli anti-tumor effective components and its pharmaceutical composition

Active Publication Date: 2013-04-03
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent 200810226062.9 "Application of Tinglizi Extract in the Preparation of Antitumor Drugs" involves the extraction solvent and efficacy of Tinglizi extract, but does not involve the specific effective parts and detailed product names of Tinglizi's anti-tumor effect

Method used

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  • Semen lepidii anti-tumor effective component extract, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1. Preparation of the anti-tumor effective component extract of Fructus Tingli

[0021] Grind the Chinese herbal medicine Tinglizi (stir-fried) and extract with 10 times the mass of water under reflux for 3 hours, repeat the extraction of the centrifuged residue once, centrifuge, combine all the water extracts, and concentrate the extract to the mass of the original medicinal material (g) 80% of the volume (ml), add 95% ethanol for alcohol precipitation, the concentration of ethanol in the liquid is about 60%~70%, let it stand overnight, centrifuge, discard the precipitate, recover ethanol under reduced pressure, add an equal volume of Ethyl acetate was extracted until the organic phase was nearly colorless, and the remaining aqueous solution was extracted 6 times with water-saturated n-butanol to recover n-butanol to obtain a water-saturated n-butanol extract. Take an appropriate amount of water-saturated n-butanol extract to pass through a silica gel column,...

Embodiment 2

[0023] Example 2. Preparation of the extract of the anti-tumor effective components of Fructus Tingli

[0024] Grind Tinglizi (stir-fried) and reflux extract with 8 times the mass of water for 2 hours, repeat the extraction of the residue after centrifugation twice, centrifuge, combine all the water extracts, concentrate the extract to the volume of the original medicinal material (grams) (ml), add 95% ethanol for alcohol precipitation, the concentration of ethanol in the liquid is about 60%, let it stand overnight, centrifuge, discard the precipitate, recover ethanol under reduced pressure, add an equal volume of ethyl acetate to extract until the organic phase is close to Colorless, the remaining aqueous solution was extracted with n-butanol until the organic phase was nearly colorless, and the n-butanol extracted fraction was collected. Take an appropriate amount of n-butanol extract to pass through a silica gel column, and perform gradient elution with different ratios o...

Embodiment 3

[0026] Example 3. Preparation of the anti-tumor effective component extract of Fructus Tinglizi

[0027] Grind Tinglizi (stir-fried) and reflux extract with 8 times the mass of water for 2 hours, repeat the extraction of the residue after centrifugation twice, centrifuge, combine all the water extracts, concentrate the extract to the volume of the original medicinal material (grams) (ml), add 95% ethanol for alcohol precipitation, the concentration of ethanol in the liquid is about 60%, let it stand overnight, centrifuge, discard the precipitate, recover ethanol under reduced pressure, add an equal volume of ethyl acetate to extract until the organic phase is close to Colorless, the remaining aqueous solution was extracted with n-butanol until the organic phase was nearly colorless, and the n-butanol extracted fraction was collected. Take an appropriate amount of n-butanol extract to pass through a silica gel column, and perform gradient elution with different ratios of dich...

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PUM

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Abstract

The invention discloses a semen lepidii anti-tumor effective component, a preparation method of the semen lepidii anti-tumor effective component and an application of the semen lepidii anti-tumor effective component in the field of anti-tumor. The preparation method comprises the following steps of: extracting semen lepidii (roasted) with water; and extracting with ethyl acetate and water saturated n-butyl alcohol sequentially; collecting water saturated n-butyl alcohol extract; separating with a silica (200 to 300 meshes) column; and performing gradient elution with dichloromethane-methanol in different proportions, wherein the dichloromethane-methanol (5:1) elution part is a semen lepidii anti-tumor effective part. The part has a remarkable effect of inhibiting U251 (glioma cell line), HepG2 (human hepatoma cell line), SGC (human gastric carcinoma cell), mcf-7 (human breast cancer cell) and SCLC (lung carcinoma cell), and can be used for preparing an anti-tumor medicament.

Description

technical field [0001] The invention relates to an extract of active antitumor components of Fructus Tingli and its pharmaceutical composition. Background technique [0002] At present, due to the intensification of environmental pollution, the acceleration of the pace of life, and the increase in the pressure of life, the incidence of cancer is on the rise. According to the latest data from the World Health Organization, the global incidence of cancer will increase by 50% by 2020. In recent years, the incidence of cancer in my country has increased at a rate of 2.5% per year, so it is more urgent to speed up the research of anticancer drugs. [0003] At present, the clinical anti-tumor drugs are mainly chemical synthetic drugs. Although the treatment of tumor diseases has been greatly improved, these anti-tumor therapeutic chemical drugs generally have the disadvantages of high toxicity and side effects, and easy drug resistance. In contrast, traditional Chinese medicine ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/31A61P35/00
Inventor 余江南奚超群徐希明童姗姗易承学曹霞
Owner JIANGSU UNIV
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