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Sheath fluid for hematology analyzer and preparation method of sheath fluid

A blood cell analyzer and sheath fluid technology, which is applied in the field of sheath fluid and its preparation, can solve the problems of inconvenient transportation and expensive reagents, and achieve the effects of good cost, convenient preparation, and avoiding damage

Inactive Publication Date: 2013-04-03
江苏美诚生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are not many companies specializing in the production of sheath fluid in China, so there are problems such as expensive reagents and inconvenient transportation.

Method used

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  • Sheath fluid for hematology analyzer and preparation method of sheath fluid

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] The present invention will be specifically introduced below in conjunction with specific embodiments.

[0020] First, the sheath fluid was prepared according to the formula shown in Table 1.

[0021] Table 1 sheath solution ratio

[0022]

tris

EDTA-2Na

Triton

1-Hydroxypyridine-2-thio

Final pH

Example 1

0.12g

0.30g

0.10g

0.10ml

7.00

Example 2

0.22g

0.30g

0.10g

0.10ml

8.45

Example 3

0.38g

0.30g

0.10g

0.10ml

9.20

Example 4

0.19g

0.50g

0.80g

0.80ml

8.40

Example 5

0.24g

0.30g

0.90g

0.10ml

8.49

Example 6

0.13g

0.10g

0.90g

0.05ml

8.42

Example 7

0.35g

0.50g

0.10g

0.01ml

8.53

Example 8

0.22g

0.30g

0.10g

0.05ml

8.47

Example 9

0.12g

0.10g

0.10g

0.80ml

8.42

Example 10

0.36g

0.50g

0.05g

0.05m...

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PUM

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Abstract

The invention discloses a sheath fluid for a hematology analyzer and a preparation method of the sheath fluid. The sheath fluid comprises the following components: a tris buffer agent, ethylene diamine tetraacetic acid, triton, 1-pyridone-2-sulphur, and the pH value of a solution formed by dissolving all the components by purified water is 7.0-9.2; and the content of each component in the sheath fluid is as follows: 0.3g / L of ethylene diamine tetraacetic acid, 0.1g / L of triton, and 0.1ml / L of 1-pyridone-2-sulphur. The sheath fluid for the hematology analyzer and the preparation method provided by the invention have the beneficial effects that few types of components are used, and general physical mixing can be adopted, thus the preparation is extremely convenient; in the formula provided by the invention, 1-pyridone-2-sulphur replaces the commonly used antimicrobial preservative sodium azide, is non-toxic, is safe to use, and causes no harm or damage to reagent producers and environments; and the sheath fluid provided by the invention can achieve the testing effect equivalent to that of the original sheath fluid, and the cost is well controlled.

Description

technical field [0001] The invention relates to a sheath liquid and a preparation method thereof, in particular to a sheath liquid for a blood cell analyzer and a preparation method thereof. Background technique [0002] The introduction of flow cytometry and the development of electronic computer technology have greatly promoted the rapid development of flow cytometry analyzers, blood cell analyzers and urine sediment analyzers, which has continuously improved the accuracy and automation of analytical instruments, and has continued to test The trend of more parameters, faster speed and simpler operation is developing. [0003] Sheath fluid is an essential reagent for the normal operation of various analyzers that use the principle of flow cytometry for various tests. Since the sheath fluid needs to wrap the sample to be tested and pass through the detection part to receive laser irradiation or electrical impedance counting, the cleanliness, absorbance, pH value, and osmoti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48
Inventor 华学山伍春宝
Owner 江苏美诚生物科技有限公司